Figure 2 | Scientific Reports

Figure 2

From: Ethanol-triggered Lipophagy Requires SQSTM1 in AML12 Hepatic Cells

Figure 2

Modulation of autophagy affects the level of ethanol-induced lipids. (A and B) AML12 cells were simultaneously incubated with ethanol and 3-MA for 24 hours. Cells were then harvested and analyzed for intracellular levels of TG (A) and cholesterol (B). (CE) AML12 cells were transfected with scramble (Scr) siRNA or ATG5-specific siRNA for 24 h, and then treated with ethanol for another 24 hours. Cells lysates were analyzed for levels of ATG5, SQSTM1, LC3, TG (D), and cholesterol (E). The protein levels were normalized to that of β-actin and expressed as fold of the control level. (FI) Cells were incubated with ethanol in the presence or absence of rapamycin (F and G) or CBZ (H,I) for 24 hours. Intracellular levels of TG (F,H) and cholesterol (G,I) were determined. Data represent mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.

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