Figure 4 | Scientific Reports

Figure 4

From: Mulberry leaf alleviates streptozotocin-induced diabetic rats by attenuating NEFA signaling and modulating intestinal microflora

Figure 4

The results of RT-PCR and western blot. (a) RT-PCR, mulberry leaf decreased PKC and increased PGC-1α and AK2 in liver tissue, and increased adiponectin expression in subcutaneous fat. *P < 0.05 compared with NCG, **P < 0.01 compared with NCG. Value = mean ± SD (N = 6). (b,c) Western blot for protein in liver tissue, mulberry leaf decreased PKC and increased OXPHOS expression. β-tubulin serves as a loading control. For presentation purposes additional lanes were excised. PKC, PGC-1α, OXPHOS and β-tubulin were cropped from different parts of the same gel. These proteins were stained one by one after using Restore™ Western Blot Stripping Buffer (Thermo Scientific, USA). Full-length gels for each protein were included in Supplementary Information Fig. S2. (d,e) Western blot for protein in subcutaneous fat, mulberry leaf increased adiponectin expression in subcutaneous fat. β-tubulin serves as a loading control. For presentation purposes additional lanes were excised. Adiponectin and β-tubulin were cropped from different parts of the same gel. Full-length gels both proteins were included in Supplementary Information Fig. S3. *P < 0.05 compared with NCG, **P < 0.01 compared with NCG. Value = mean ± SD (N = 3–4).

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