Figure 6 | Scientific Reports

Figure 6

From: Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2

Figure 6

Ratiometric FRET imaging of Gq-activation biosensors equipped with novel FRET pairs. FRET ratio-imaging was performed on Hela cells over-expressing the histamine−1 receptor and a FRET biosensor for Gq activation. The blue, solid lines show the mTurquoise2 fluorescence intensity over time, the dashed lines show the acceptor emission level over time. The initial fluorescence intensity is normalized to the average intensity of the first 5 frames. The black graph in a separate upper right window shows the FRET ratio over time. The thin lines indicate the 95% confidence intervals. 100 μM histamine was added after 42–50 s (black arrowhead) and 10 μM pyrilamine was added after 140–150 s (grey arrowhead). The number of cells analysed is: Gqsensor-mTq2-mNeonGreen n = 32 (out of 34 in total), Gqsensor-mTq2-SYFP2 n = 42 (out of 44 in total), Gqsensor-mTq2-mScarlet-I n = 24 (out of 26 in total) and Gqsensor-mTq2-mCherry n = 19 (out of 26 in total)

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