Inner cell Mass (ICM) specification into epiblast (Epi) and primitive endoderm (PrE) is an asynchronous and progressive process taking place between E3.0 to E3.75 under the control of the Fibroblast Growth Factor (FGF)/Extracellular signal-Regulated Kinase (ERK) signaling pathway. Here, we have analyzed in details the kinetics of specification and found that ICM cell responsiveness to the up and down regulation of FGF signaling activity are temporally distinct. We also showed that PrE progenitors are generated later than Epi progenitors. We further demonstrated that, during this late phase of specification, a 4 hours period of FGF/ERK inhibition prior E3.75 is sufficient to convert ICM cells into Epi. Finally, we showed that ICM conversion into Epi in response to inhibition during this short time window requires both transcription and proteasome degradation. Collectively, our data give new insights into the timing and mechanisms involved in the process of ICM specification.
During early mammalian development, two distinct differentiation steps occur during the formation of the blastocyst. The first one will generate the trophectoderm and the inner cell mass (ICM) followed by the specification of ICM cells into the epiblast (Epi) and the primitive endoderm (PrE). These events are highly coordinated and regulated by a limited number of transcription factors and cell signaling. Epi/PrE formation can be viewed as a three-step model1. First, blastomeres initially co-express the Epi marker NANOG and the PrE marker GATA6 until E3.25 (32-cells)2. Specification of both Epi and PrE is thought to occur asynchronously between E3.25 to E3.75 (64-cells) which is reflected by an ICM composition of cells expressing either NANOG or GATA63. These two cell populations ultimately reorganize by a cell sorting process and, by E4.5 (>100 cells), the PrE forms a single cell layer in contact to the blastocoel cavity2,4.
NANOG and GATA6 transcription factors are two key-lineage markers of Epi and PrE formation respectively and have been proposed to mutually repress each other. Indeed, all ICM cells adopt a PrE fate in Nanog mutant embryos5 while a reverse situation is observed in Gata6 mutants6,7.
Fibroblast Growth Factor (FGF)/Extracellular signal-Regulated Kinase (ERK) signaling pathway is considered as the main regulator of Epi/PrE lineage decision. Genetic inactivation of several members of the FGF pathway including Grb2 3, Fgfr1/2 8,9 and Fgf4 10,11 impairs PrE formation. Similarly, pharmacological perturbation of FGF/ERK activity also strongly affects PrE/Epi specification12,13. Indeed, when FGFR or MEK is inhibited, all ICM cells adopt an Epi fate. Reciprocally, ICM cells specify into PrE when embryos are cultured in presence of high dose of FGF4. ICM cell plasticity and responsiveness to modulations of FGF activity is progressively lost and around E4.0 cell lineages are determined13,14,15.
In this study, we have examined in a greater temporal resolution the consequences of modulating FGF/ERK activity during the process of ICM cell specification. We first show that, while being a gradual process, Epi progenitors emerge faster than PrE progenitors. We also refined the windows of ICM cell sensitivity to the modulation of FGF/ERK activity. Particularly, we identified a narrower time window (≤4 h) between E3.5 and E3.75 when ICM cells are particularly sensitive to FGF/ERK inhibition. We propose that FGF signaling might act on PrE/Epi specification later than previously thought at the time when Epi progenitors have been formed but PrE progenitors are still emerging. Lastly, we show that both transcriptional and post-translational regulations are required for ICM conversion to Epi upon FGF/ERK inhibition.
Sensitivity to FGF/ERK up and down regulation are temporally distinct
Several reports investigated the impact of FGF/ERK signaling modulation on Epi/PrE specification12,13,15. While in absence of FGF activity all ICM cells adopted an Epi fate (NANOG-positive only), a complete reverse situation was observed when FGF signaling was stimulated so that all ICM cells became PrE (GATA6-positive only). Here, we undertook a detailed analysis of the dynamics of ICM cell sensitivity to FGF/ERK modulation during blastocyst formation (i.e from E2.75 to E4.5). To that end, embryos were recovered and cultured from E2.75 to E3.25, E3.25 to E3.75 and E3.75 to E4.5 in presence of either a combination of FGF receptor (PD173074) and MEK (PD0325901) inhibitors or FGF4 ligand. Embryos were next processed to determine by immunofluorescence the proportion of NANOG-positive (Epi progenitors), GATA6-positive (PrE progenitors) and co-expressing ICM cells (Fig. 1). During this time course, we observed the progressive specification of ICM cells as visualized by the reduction of NANOG/GATA6 co-expressing cells (72.4 ± 2.8% at E3.25 (Fig. 1A), 9.3 ± 2.8% at E3.75 (Fig. 1B) and 0% at E4.5 (Fig. 1C)) and confirmed that the salt-and-pepper pattern is mainly established between E3.25 and E3.75 leading to an ICM composition of 40% Epi and 60% PrE at E4.5 as described in ref.15.
Upon FGF stimulation, we observed a strong impact on the ICM specification between E2.75 and E3.25 (Fig. 1A) but little (E3.25 to E3.75, Fig. 1B) or no effect (E3.75 to E4.5, Fig. 1C) at later stages. Indeed, at the earliest stages, ICM cells were prematurely specified (72.4 ± 2.8% vs. 23.3 ± 3.9% of co-expressing cells in control and FGF4-treated embryos) into PrE progenitors (11 ± 2.3% vs. 72 ± 3.7% of GATA6-positive cells in control and FGF4-treated embryos) and at the expense of Epi progenitors (16.5 ± 2.2% vs. 4.7 ± 1.7% of NANOG-positive cells in control and FGF4-treated embryos). We conclude that, as Epi/PrE specification proceeds, ICM cells loose their sensitivity to exogenous FGF stimulation.
On the contrary, FGF/ERK inhibition had no effect between E2.75 and E3.25 (Fig. 1A) but strongly impacted on Epi/PrE specification from E3.25 with a bias towards Epi identity (48 ± 2.7% vs. 100% (E3.25 to E3.75) and 46 ± 2% vs. 77.2 ± 6.1% (E3.75 to E4.5) NANOG-positive cells in control vs. treated embryos). Thus, the highest sensitivity to FGF/ERK inhibition was observed during E3.25 to E3.75 and slightly decreased between E3.75 to E4.5.
Taken together, our data identify two developmental time windows of inner cell sensitivity to modulation of FGF/ERK activity: the first one from E2.75 to E3.25 when ICM cells are sensitive to exogenous FGF stimulation but insensitive to FGF inhibition and a second from E3.25 to E4.5 when the inner cells present a reverse sensitivity to the modulation of FGF activity. Thus, ICM cell responsiveness to the up and down regulation of FGF signaling activity are temporally distinct. Interestingly, the period of highest sensitivity to FGF inhibition (E3.25 to E3.75) coincides with the timing of establishment of the salt-and pepper pattern.
Epi progenitors are specified before PrE progenitors
Having established that, between E3.25 and E3.75, a majority of co-expressing ICM cells progress to a mutually exclusive NANOG/GATA6 expression state, we thought to analyze in greater details the kinetics of emergence of these two, Epi and PrE, progenitor cell populations. We collected E3.25 blastocysts and determined the number of co-expressing (NANOG- and GATA6-positive) ICM cells, NANOG-positive Epi progenitors and GATA6-positive PrE progenitors over an 8 hours period of culture (Fig. 2A). Co-expressing ICM cells dropped down from 77% to 10% after 8 hours of culture confirming that most of the ICM cells have been specified during this time window (Fig. 2B,C). Interestingly, during the first 4 hours, the number of Epi progenitors increased rapidly to reach 50% of inner cells. At later time points, no or very little Epi progenitors were produced. Surprisingly, compared to Epi progenitors, production of PrE progenitors was late with a majority of them being produced during the last 4 hours. To confirm our findings, we analyzed the dynamics of SOX17, an additional early PrE marker (Artus et al., 2011; Niakan et al., 2010), during the same timing of culture. We found that the proportion of SOX17-positive, NANOG-negative cells increases mostly during the last 4 hours (Fig. 2D,E). Contrasting with GATA6 stainings, double (SOX17-, NANOG-) -negative ICM cells were observed, which is consistent with our previous observation that Sox17 shortly follows Gata6 expression (Artus et al., 2011). Collectively, our data suggest that, while Epi/PrE specification is a gradual process, PrE progenitors tend to be specified later than Epi progenitors.
ICM cells are sensitive to FGF/ERK modulation during late phase of Epi/PrE cell specification
Our observations that during E3.25 and E3.75, most of ICM cells are specified and exhibit high sensitivity to FGF/ERK inhibitors prompted us to examine in greater details their effect during this time window. We monitored NANOG and GATA6 expression in E3.25 embryos treated for 2, 4, 6 and 8 hours (Fig. 3A). Strikingly, 2, 4 and 6 hours of treatment had no major impact on ICM cell specification (Fig. 3B,C) since their inner cells composition appeared similar to that of control embryos (compare Figs 2C and 3C). In particular, GATA6-positive inner cells were still observed in treated embryos. It was only after 8 hours of FGF/ERK signaling inhibition that ICM cells uniformly adopted an Epi fate. Our results suggest that duration of FGF/ERK inhibition might be important to achieve complete ICM conversion to Epi. Alternatively, ICM cells may be sensitive to FGF/ERK inhibition only at a late stage of ICM specification.
To further address these issues, we cultured embryos from E3.25 to E3.75 and added inhibitors during the last 2, 4 or 6 hours of culture (Fig. 4A). Two hours of inhibition were not sufficient to downregulate GATA6 expression in all ICM cells. In contrast, inhibition of FGF/ERK signaling for the last 4 or 6 hours of culture was sufficient to make almost all inner cells expressing only NANOG (Fig. 4B,C). Remarkably, while a 4 hours treatment did not affect ICM specification when applied early (Fig. 3C), it had a major impact when applied late (Fig. 4C). This observation suggests that the effect of FGF/ERK inhibition on ICM cell fate largely depends on the developmental period when inhibitors are applied rather than the duration of the treatment.
Even though FGF stimulation had a moderate impact on PrE/Epi cell specification between E3.25 and E3.75 (Fig. 1B), we asked whether ICM cells exhibited differential sensitivity to FGF stimulation (Fig. S1A). We observed a significant bias towards PrE fate at the expense of Epi only when the treatment was applied during the last 4 hours of that period (Fig. S1B,C). Interestingly, only the number of NANOG-only ICM cells was slightly reduced compared to control (Fig. S1Cd) and corresponded to the number of Epi progenitors already present at the onset of FGF4 treatment (Fig. S1Ba). This suggests that FGF4 treatment blocked unspecified ICM cells towards Epi identity rather than affected the fate of already specified Epi progenitors.
Collectively, our data show that, during the time of Epi/PrE specification (i.e between E3.25 and E3.75), the late phase, which corresponds to the time when Epi progenitors have been formed and PrE progenitors are still emerging, is more sensitive to the modulation of FGF/ERK signaling activity than the early phase.
Mutually exclusive NANOG/GATA6 expression can occur in absence of transcription or proteasome degradation
Our data show that ICM cells can be instructed towards an Epi fate in a narrow time window (4 hours or less) implying rapid and dynamic regulation of GATA6 and NANOG levels. We thus analyzed the contribution of transcription and proteasome degradation on ICM cell specification during that period with and without FGF/ERK inhibition. Embryos were cultured from E3.25 to E3.75 and treated with flavopiridol, a potent RNA Polymerase II inhibitor16 or with the proteasome inhibitor MG132. Treatment was initiated one hour before the addition of FGF and MEK inhibitors and maintained until the end of the culture (Figs 5A and S2). We first confirmed that the 1 h pretreatment with flavopiridol (but not with MG132) inhibited transcription as visualized by the reduction of Rps17 pre-mRNA (Fig. S2A) and did not affect ICM composition (Fig. S2B). After 5 hours, flavopiridol treatment led to a marked reduction of both Rps17 pre- and mature mRNA while MG132 treatment affected the level of Rps17 pre-mRNA only.
Next, we monitored the consequences of flavopiridol or MG132 treatment on embryonic development. In the presence of flavopiridol, embryos showed a clear reduction of ICM cell number (Fig. 5C) as a probable consequence of increased cell death (Fig. 5B, arrowheads). While presenting no signs of increased cell death, MG132-treated embryos also exhibited a reduction of ICM cell number (Fig. 5C) likely caused by a block in mitotic progression. Indeed, MG132 treated embryos exhibited a high number of metaphases (Fig. 5B, arrows) reflecting the known function of the anaphase-promoting complex in targeting mitotic proteins for degradation by the proteasome17. Inhibition of neither RNA polymerase II or proteasome degradation prevented the establishment of NANOG and GATA6 exclusive expression by inner cells (Fig. 5C), suggesting that the treatment did not dramatically interfere with ICM specification.
We observed a slight increase of the proportion of Epi cells at the expense of PrE cells in presence of flavopiridol. Interestingly, flavopiridol treatment resulted in a 20% decrease in GATA6 levels in PrE progenitors while NANOG levels in Epi progenitors remained unchanged (Fig. 5D,E). This is consistent with previous reports showing that a significant but moderate reduction of GATA6 in PrE cells in Gata6 +/− embryos leads to an imbalance PrE/Epi specification6,7. Together, our data show that acquisition of PrE fate critically requires active transcription during the late phase of specification.
In contrast, MG132 treatment did not affect the NANOG+/GATA6+ inner cells ratio. Nonetheless, strong reduction in GATA6 levels (40%) in PrE progenitors and in NANOG levels (80%) in Epi progenitors were observed in embryos treated with MG132 for 5 hours (Fig. 5B,D,E). A decreased in NANOG levels in Epi progenitors was already observed after 1 hour of MG132 treatment (Fig. S2C). Thus, our data show that proteasome activity positively regulates the levels of both transcription factors during that period pointing to additional regulatory mechanisms that remain to be discovered. The fact that NANOG levels in co-expressing ICM cells was hardly affected by MG132 treatment (Fig. S2D) further suggests that such mechanisms are modulated while ICM specification proceed.
We also noticed the presence of GATA6-positive cells in the trophectoderm of MG132-treated embryos, a situation which was rarely observed in control or flavopiridol-treated embryos at late blastocyst stage (Fig. 5B). This observation suggests that GATA6, but not NANOG, levels are negatively regulated by the proteasome in the trophectoderm cell lineage.
ICM conversion to Epi following FGF/ERK inhibition requires both transcription and proteasome activity
We next analyzed the role of transcription and proteasome degradation on the action of FGF and MEK inhibitors on Epi/PrE specification. In this paradigm, treated embryos are thought to up-regulate NANOG levels and down-regulate GATA6 so that, in fine, all ICM cells become exclusively NANOG-positive. Embryos were pretreated for 1 h with flavopiridol or MG132 before addition of the inhibitors to the culture medium for 4 additional hours (Fig. 5A). Interestingly, both treatments impaired the effect of FGF/ERK inhibition. Indeed, while control embryos incubated with FGF/ERK inhibitors contained almost exclusively NANOG-positive inner cells, flavopiridol- and MG132-treated embryos contained GATA6 expressing inner cells and essentially resemble to embryos treated with drugs but no inhibitors (Fig. 5B,C). The higher proportion of double-positive cells in embryos treated with MG132 and FGF/ERK inhibitors compared to MG132 alone (40.8 ± 6.3 vs 19.7 ± 5.5, p < 0.005, Fig. 5C) may be due to the upregulation of NANOG expression in PrE progenitors upon FGF/ERK inhibition together with incomplete downregulation of GATA6 in absence of proteasome activity.
Consistent with the role of FGF/ERK signaling on GATA6 expression3,10, we found reduced GATA6 levels in PrE cells from embryos treated with FGF/ERK inhibitors (Fig. 5E). Absence of further reduction in presence of flavopiridol or MG132 suggests that FGF/ERK regulates GATA6 levels at both transcriptional and posttranscriptional levels. It has been previously reported that FGF/ERK inhibition leads to marked upregulation in NANOG levels in Epi of E4.5 (>100 cells) embryos7. In E3.75 embryos treated with FGF/ERK inhibitors, we found no or modest upregulation in NANOG levels in Epi progenitors and co-expressing ICM cells respectively (Figs 5D and S2D) indicating that ICM conversion to Epi does not require deregulated NANOG levels and that FGF/ERK signaling likely controls NANOG levels in Epi after specification. In ES cells, FGF/ERK signaling has been shown to directly repress Nanog transcription18. During specification of ICM cells, the link between FGF/ERK signaling and Nanog transcription is likely different since NANOG levels were reduced in Epi progenitors of embryos treated with FGF/ERK inhibitors and flavopiridol but not with flavopiridol alone (Fig. 5D).
Collectively, our data show that FGF/ERK inhibitor activity on ICM cell conversion is both dependent on transcription and proteasome degradation.
In this study, we investigated the timing of ICM cell specification into Epi and PrE cell fate and observed that while being a gradual process, the specification of Epi progenitors precedes PrE progenitors (Fig. 6). This is maybe not surprising since PrE specification depends on FGF4 ligand, which is assumed to be secreted by Epi cells once specified19. Importantly, our study redefines the windows of competence during which ICM cells can respond to experimental modulation of FGF/ERK signaling activity. Lastly, we propose that the effect of FGF/ERK inhibition on ICM cells requires transcription and protein degradation.
Developmental time-dependent sensitivity to FGF modulation
Lineage tracing experiments have shown that ICM cells are committed to either Epi or PrE cell fate3. Several studies have addressed the key role of FGF signaling in the control of Epi/PrE cell specification13,14,15. However, it is not entirely clear how uncommitted ICM cells (NANOG and GATA6-positive cells) behave upon FGF modulation during this specification process particularly whether all (or a subset) of uncommitted ICM cells can respond at any time to FGF modulation. We proposed from our data that the competency of uncommitted ICM cells to respond to FGF signaling is rapidly evolving with time. Indeed, we have identified four distinct periods where ICM cells exhibit distinct behaviors in response to FGF modulation (Fig. 6). From E2.75 to E3.25, ICM cells are sensitive to exogenous FGF stimulation as previously described6,13. This suggests that while ICM cells exhibit heterogeneous levels of FGF transduction machinery (Fgf4/Fgfr2)20,21, all of them can respond to increased levels of FGF activity. Consistent with previous studies on E3.25 Fgf4 mutants10,11,21, ICM cells are not affected by FGF/ERK inhibition during that period indicating that NANOG and GATA6 levels in ICM cells are not tightly regulated by FGF signaling at this stage. This period is followed by a stage (from E3.25 to E3.5) where ICM cells are still insensitive to FGF/ERK inhibition but lost their ability to respond to exogenous FGF stimulation. The reason for this is currently unclear. Whether this is due to dynamic regulation of components of the signaling cascade or intrinsic variations of the cell ability to respond to stimulation such as the phase of the cell cycle will require further investigations. From E3.5 to E3.75, ICM cells are highly sensitive to FGF/ERK inhibition as demonstrated by our observation that a short 4 h treatment is sufficient to drive almost all ICM cells towards Epi cell fate (Fig. 4). However in the same period, ICM cells poorly respond to FGF stimulation. From E3.75, their ability to respond to modulation of FGF activity is progressively lost.
The mechanisms underlying the acquisition of such competence are currently unknown. One possibility could be that ICM cells with different sensitivity to FGF/ERK inhibition coexist within the ICM and are specified with different timing. Responsive ICM cells would be bias towards PrE fate and because they specify late, FGF/ERK inhibition at early timing would have no immediate impact on ICM composition. It has been proposed that inner cells generated during the second wave of asymmetric division express higher levels of Fgfr2 and are biased towards PrE fate22,23. However, whether these cells are specified later than inner cells generated from the first wave has not yet been determined. Another possibility would be that ICM will first give rise to Epi progenitors, as predicted by a mathematical modeling of ICM differentiation and confirmed in situ 6, that in turn would modify the remaining ICM cells and make them responsive to FGF/ERK signaling. The accumulation of FGF4 which is secreted by Epi cells is likely to mediate this switch as previously proposed24. Above a certain threshold, which would be attained only when most Epi cells are specified, high FGF4 levels would trigger robust activation of FGF/ERK in remaining ICM cells and drive them towards PrE differentiation.
Cell fate plasticity within Epi and PrE progenitor populations
Lineage tracing and chimera experiments have suggested that Epi progenitors exhibit less plasticity than PrE precursors14,15,25. Our data also argue in favor of a model whereby, as they emerge, Epi progenitors loose their ability to be converted into PrE upon FGF stimulation. Indeed, embryos treated with FGF4 from E3.25 to E3.75 showed a significant bias towards PrE fate at the expense of Epi (Fig. 1B). While the number of NANOG-only ICM cells was reduced compared to control (9.8 ± 0.8 vs. 5.2 ± 1.0 NANOG-positive cells in control vs. treated embryos, Fig. 1Ba-b), it remains closer to the number of NANOG-only ICM cells present in E3.25 embryos before treatment (2.7 ± 0.5 NANOG-positive cells, Fig. 1Aa). This suggests that FGF4 treatment prevented unspecified ICM cells to adopt Epi identity rather than affected the fate of already specified Epi progenitors. Between E3.5 and E3.75, FGF4 treatment had a mild impact on ICM composition so that 7.9 ± 1.1 ICM cells remained Epi progenitors (compared to 9.8 ± 0.8 cells in control conditions). Lastly, by E3.75, FGF4 treatment does not affect the composition of the ICM indicating that NANOG-positive cells are unlikely to be converted into PrE progenitors upon exogenous FGF stimulation.
Conversely, the ability of PrE progenitors to be converted into Epi in response to FGF inhibition seems to be modulated in time. This is exemplified by our observation that inhibition of FGF/ERK from E3.5 to E3.75 leads to an ICM almost exclusively composed of Epi progenitors (Fig. 4) while similar treatment has a less profound effect when applied from E3.75 to E4.5 (Fig. 1C).
Taken together our data fit with the current model of ICM cell specification and cellular plasticity and future studies using live imaging to address the temporal dynamics of these single and double-positive populations will undoubtedly give precious insights into these questions.
Regulation of ICM cell specification by transcription and proteasome degradation
While several studies have addressed the interplay between NANOG, GATA6 and FGF signaling, it is still not clear how GATA6/NANOG positive uncommitted ICM cells mature into single positive cells and how FGF/ERK inhibition impacts on this process so that almost all cells are NANOG- positive only. Our data indicate that inhibition of proteasome-mediated proteolysis (or transcription) has a low impact on the establishment of GATA6/NANOG mutually exclusive expression from co-expressing ICM cells, while it severely impaired the effect of FGF/ERK inhibition.
Unexpectedly, inhibition of the proteasome led to a strong reduction of NANOG levels in Epi progenitors. This is surprising since NANOG protein stability in pluripotent stem cells has been shown to be tightly regulated through its PEST domain26, ERK-mediated phosphorylation27,28, interaction with stabilizing proteins such as the COP9 signalosome member COPS229 or the deubiquitinase USP2130. Accordingly, MG132 treatment in ES cells leads to increased NANOG levels26,28,29,31. Thus, our data reveal that NANOG levels are differentially regulated between Epi progenitors and pluripotent stem cells and point to additional mechanisms controlling NANOG stability in vivo during ICM cell specification. A likely possibility would be that an inhibitor of NANOG expression or stability is present in Epi progenitors and actively degraded by the proteasome between E3.5 and E3.75. It has been proposed that, among others32,33, GATA6 is an important regulator of NANOG34,35,36. Further experiments will be required to test its implication in vivo in ICM cells.
Although less pronounced than for NANOG, a positive regulation of GATA6 levels by the proteasome was observed in PrE progenitor. It would be interesting to determine the mechanisms regulating GATA6 stability and to test possible candidates such as interaction with the polycomb member BMI137, destabilization of Gata6 mRNA by UNR38 or cAMP-dependent proteolysis that have been previously shown to regulate GATA6 levels in other contexts39,40. A large number of evidences have pointed out the role of proteasome activity in stem and progenitor cells41. In addition, other positive regulation on protein levels by the proteasome has been documented in various contexts, including through factors regulating the translation and the stability of mRNA during oocyte maturation42. In general, a high dynamic regulation of the protein stability may be important to rapidly modulate cell fate decision in response to external cues.
Our data also show that contrary to NANOG levels in Epi, maintenance of high levels of GATA6 in PrE requires active transcription during the period when these progenitors are responsive to FGF/ERK inhibition (Fig. 5D,E). Importantly, while inhibition of FGF/ERK signaling caused the complete downregulation of GATA6 in PrE progenitors already present in the embryos at the onset of the treatment, this was no longer the case when transcription was blocked. Similarly, blocking proteasome degradation prevent the conversion of ICM into Epi cell lineage upon FGF/ERK inhibition. Thus, our data show that the regulation of GATA6 levels by FGF signaling in PrE progenitors by the time of specification is manifold and acts at several levels including transcription and protein stability.
Taken together, our data shed light on the mechanisms involved in Epi/PrE specification which for the first time address the roles of transcription and proteasome.
Material and Methods
Embryo collection and culture
Embryos from CD1 (Charles River laboratories, France) intercrosses were recovered at E2.75 and at E3.25 (expanding blastocyst) by flushing oviducts or uteri in M2 (Millipore), washed twice and cultured inside their intact zona pellucida in 400 µL KSOM (Millipore) in Nunc 4-well plates until the stages of interest at 37 °C, 8% CO2. For treatment, we used FGF receptor inhibitor PD173074 (100 nM, Sigma Aldrich), MEK inhibitor PD0325901 (1μM, Sigma Aldrich), flavopiridol (1μM, Selleckchem), MG132 (10 μM, Sigma Aldrich) and FGF4 (1 μg/ml, R&D systems) supplemented with heparin (1 μg/ml, Sigma Aldrich). Experiments shown in Figs 2(A–C) and 3 were performed in parallel using same batches of embryos. All experiments were conducted according to the French and European regulations on care and protection of laboratory animals (EC Directive 86/609, French Law 2001–486 issued on June 6, 2001) and were approved by the Institut Pasteur ethics committee (n° 2012–0011).
Embryos were fixed in 4% PFA overnight at 4 °C and then washed three times in PBT (PBS, 0.1% Triton X-100). Embryos were preincubated in blocking solution (PBT, 10% FBS) during 1 hour and then incubated overnight at 4 °C. In this study, anti-GATA6 (1/100, AF1700, R&D Systems), anti-CDX2 (1/100, MU392A-UC, Biogenex), anti-NANOG (1/100, 8822, Cell Signaling; 1/100, 14–5761, eBioscience), and anti-SOX17 (1/100, AF1924, R&D systems) were used. After several washes in PBT, embryos were incubated with secondary antibodies coupled with Alexa 488 nm, 546 nm, 647 nm (1/300, Invitrogen) and Hoechst (1.6 µM, Sigma Aldrich) 2 hours at room temperature and then washed in PBT. We manually counted the number of cells positive only for GATA6/SOX17/NANOG or double positive (GATA6 or SOX17/NANOG). Embryos were and analyzed using a SP5 (Leica) or LSM800 (Zeiss) confocal microscope. Images were acquired using glass bottomed microwell dishes (MatTek Corporation) using the same objective (Plan-apochromat 20 × /NA 0.75), speed (700 Hz), pinhole 1 airy unit, and laser intensities with optical section thickness of 1.2–1.4 μm. 16-bit resulting images were analyzed and eventually processed using Icy, Fiji and Photoshop CS5 softwares.
3D stacks were analyzed using Icy software43 (http://icy.bioimageanalysis.org/protocol/3D_mouse_embryo_quantification), for which we designed a custom semi-automated analysis protocol available here. After selecting the nuclei staining channel, the protocol applies a wavelet decomposition of the 3D image volume to segregate image structures based on size and intensity. After optimizing the selection of wavelet scales and their respective thresholds, the protocol extracts one or more 3D regions of interest (ROI) for each nucleus. The selection of scales and thresholds is adapted to each embryo, depending on the signal-to-noise ratio. Finally, the protocol super-imposes the extracted ROI on the original data for visual inspection. In few instances, multiple ROI are detected inside the same nucleus, and are merged manually post-analysis. The mean of fluorescence intensity for GATA6 and NANOG channels for each ROI is then calculated.
Blastocyst gene expression profile
mRNAs of 5 pooled blastocysts were isolated using RNeasy Plus Micro kit (Qiagen) according manufacturer instructions, reverse transcribed using Superscript VILO (Thermo Fischer Scientific) and analyzed using SYBR Green PCR Master Mix (Applied Biosystems). Mouse RT-qPCR primers were designed using Primer Blast (NCBI): Rps17-F 5′-CGCCATTATCCCCAGCAAGA-3′, Rps17-R 5′ GCTGAGACCTCAGGAACGTAG-3′, Pre-mRNA-F 5′-TTTGTTGGTGTGTCCTGGCT-3′ and Pre-mRNA-R 5′-CCACCCGGCTAATGAACACT-3′.
Graphs and statistical tests (t-test and Mann-Whitney) were performed using Prism (Graphpad).
Raw data for embryo cell counts, lineage assignments and for quantitative immunofluorescence analyses are supplied as Supplementary Table S1.
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Imaging was performed for most parts at the Imagopole of Institut. This work was supported by the Institut Pasteur, the Centre National de la Recherche Scientifique and the Agence Nationale de la Recherche (ANR-10-LABX-73-01 REVIVE and ANR-14CE11-0017 PrEpiSpec). S.B. was supported by the Fondation pour la Recherche Médicale (ARF20150934222). J.A. was supported by the European program Marie Curie (International Incoming Fellowship, Seventh European Community Framework Programme).
The authors declare that they have no competing interests.
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Bessonnard, S., Coqueran, S., Vandormael-Pournin, S. et al. ICM conversion to epiblast by FGF/ERK inhibition is limited in time and requires transcription and protein degradation. Sci Rep 7, 12285 (2017). https://doi.org/10.1038/s41598-017-12120-0
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