Postulated role of miRNA in APAP toxicity. Schematic illustration of miRNA regulating CYP 450 in APAP toxicity. miRNAs (miR-122, miR-378a, miR-27b and miR-125b) were detected in cell culture medium and serum from APAP over dose subjects. CYP1A2, CYP3A4 and CYP2E1 were detected by qPCR in HepaRG cells and mRNA expression levels match the color coated keys in Fig. 1E,F. Dashed lines show miRNA predicted to target 3′ UTR of CYPs. The bold lines show validation with gain of function experiments for miR-122 and miR-378a mimics in a cellular model.