Figure 2 | Scientific Reports

Figure 2

From: MicroRNA regulation of CYP 1A2, CYP3A4 and CYP2E1 expression in acetaminophen toxicity

Figure 2

Luciferase assay confirming direct suppression of CYP1A2 and CYP3A4 transcription through 3′UTR binding of miR-122. Effects of miR-122 mimic or inhibitor on luciferase activity in HEK293 cells co-transfected with a firefly luciferase reporter vector containing the CYP1A2 3′UTR-Luc (Fig. 2A) and CYP3A4 3′ UTR-Luc (Fig. 2B). Control plasmid pEZX-MT06 with no 3′UTR, miR-122 mimic (1 nM and 5 nM), miR-122 inhibitor (100 nM). A one-way analysis of variance (ANOVA) with post hoc Dunnett’s test, *p < 0.0196 and **p < 0.006. Values are mean of 2 independent experiments. RLU = relative luciferase units.

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