Effect of gut decontamination. Effect of antibiotic treatment on body weight (a), hepatic triglyceride deposition (b) and adipose tissue inflammation (c). Mesenteric lymph nodes colonization was decreased after antibiotics (d) and was associated with a significant reduction of hepatic TLR4, TLR5 and TLR9 gene expression (e). Antibiotics reduced hepatic gene expression of F4/80, MCP-1 and Type I collagen (f) and NAS Score (g) in Nlrp3−/−-HFHC mice. Mean ± SE: Mean ± SE: *p < 0.05; **p < 0.01; ***p < 0.001; #p < 0.05 vs Nlrp3−/−-Chow diet; ##p < 0.01 vs Nlrp3−/−-Chow diet; ###p < 0.001 vs Nlrp3−/−-Chow diet; ####p < 0.0001 vs Nlrp3−/−-Chow diet; çp < 0.05 vs Wt-Chow diet; ççp < 0.01 vs Wt-Chow diet; ùp < 0.05 vs Wt-HFHC; ùùp < 0.01 vs Wt-HFHC; ùùùp < 0.001 vs Wt-HFHC; §p < 0.05 vs Nlrp3−/−-HFHC; §§p < 0.01 vs Nlrp3−/−-HFHC; §§§p < 0.001 vs Nlrp3−/–HFHC.