Adipose tissue inflammation was higher in Nlrp3−/−-HFHC mice. Percentage of fat mass was increased in Nlrp3−/−-HFHC (a). MAC-2 immunohistochemistry (magnification, 40×) showed adipose tissue inflammation in Nlrp3−/−-HFHC mice (b). Gene expression of adipose tissue TNF-α (c) and MCP-1 (d) evaluated by qRT-PCR was also increased in Nlrp3−/−-HFHC mice. Mean ± SE: *p < 0.05; **p < 0.01; ***p < 0.001.