Local Juvenile Hormone activity regulates gut homeostasis and tumor growth in adult Drosophila


Hormones play essential roles during development and maintaining homeostasis in adult organisms, regulating a plethora of biological processes. Generally, hormones are secreted by glands and perform a systemic action. Here we show that Juvenile Hormones (JHs), insect sesquiterpenoids synthesized by the corpora allata, are also synthesized by the adult Drosophila gut. This local, gut specific JH activity, is synthesized by and acts on the intestinal stem cell and enteroblast populations, regulating their survival and cellular growth through the JH receptors Gce/Met and the coactivator Tai. Furthermore, we show that this local JH activity is important for damage response and is necessary for intestinal tumor growth driven by activating mutations in Wnt and EGFR/Ras pathways. Together, our results identify JHs as key hormonal regulators of gut homeostasis and open the possibility that analogous hormones may play a similar role in maintaining vertebrate adult intestinal stem cell population and sustaining tumor growth.


Juvenile Hormones (JHs) are versatile hormones, playing major roles during larval development and in adult insects1, 2. The most characterized role of JHs is to maintain the larval status between molts after periodic pulses of 20-hydroxyecdysone3. In adults, JH modulates many biological processes, such as ovarian maturation, behavior, caste determination, diapause, stress response and life span, among others1, 2, 4. Moreover, it has been recently shown that an increase in systemic JHs occurs in females after mating, which induces gut remodeling and proliferation of the intestinal stem cell (ISC) population5. These pleiotropic effects of JHs are regulated through multiple pathways.

The adult gut of Drosophila is maintained by a population of ISCs. ISCs divide asymmetrically giving rise to another ISC and either an endocrine cell (EE) or an enteroblast (EBs), which in turn differentiates into an enterocyte (ECs)6,7,8,9. Several signaling pathways such as Notch, Wnt, Jak/STAT, JNK, Nrf2 or Hippo, among others, regulate ISCs self-renewal and proliferation both in normal conditions and in response to damage or injury, ensuring intestinal homeostasis10,11,12,13,14. Moreover, misregulation of some of these pathways leads to tumor growth. For example, stem cell-derived clones mutant for the Wg negative regulators Apc and Apc2 and overexpressing the oncogenic form of Ras, RasV12 (hereafter referred as Apc-Ras clones) form overgrowths that show many tumor characteristics15, 16.

JHs are acyclic sesquiterpenoids known to be synthesized by the corpus allatum (CA), a pair of endocrine glands integrated in the ring gland of insects, which in Drosophila is located above the brain hemispheres with its dorsal portion tilted anteriorly3, 17. Several genes codify for enzymes required for the biosynthesis of JHs, including Juvenile hormone acid o-methyltransferase (jhamt). jhamt encodes for an enzyme which generates active JHs by transferring a methyl group from S-adenosyl-L-methionine to the carboxyl group of JHs acids at the latest steps of JH biosynthesis18, 19. The titer of JH is precisely regulated by many physiological and biochemical processes. Its synthesis is controlled by the nervous system through the secretion of stimulatory and inhibitory neuropeptides (allatotropins and allatostatins respectively)20. JHs interact with two bHLH-PAS proteins, Methoprene-tolerant (Met) and its paralog germ cell-expressed (Gce), which act as its receptors21. JH binding induces the recruitment of the transcription factor Taiman (Tai) to DNA22, triggering the expression of JH-responsive genes such as Krüppel homolog 1 (Kr-H1)3.

Here we describe the adult gut populations of ISCs and EBs as a new source of JHs in Drosophila. This local, gut-specific JH production initiates an autocrine loop required for cellular growth and survival of the progenitor cell population in a Met/Gce and Tai-dependent manner. In addition, we show that Apc-Ras-induced tumors fail to grow and survive in the anterior midgut of the adult fly when the gut-specific JH activity is compromised. These results open the interesting possibility that vertebrate adult intestinal stem cells may also be regulated by local hormonal cues, regulating both normal tissue homeostasis and tumor growth.


JHAMT expression is required for the survival of aging progenitor cells

In order to identify new regulators of adult ISC homeostasis, we performed an in vivo RNAi screen based on previously selected candidate genes (unpublished work). We expressed each RNAi line upon the Escargot-Gal4 (esg-Gal4) driver, which is specifically expressed in the ISC and EB populations of the adult midgut6, 7, controlling the temporal expression by a temperature sensitive allele of Gal80 (Suppl. Fig. 1A). Upon four weeks at permissive temperature, jhamt RNAi expression was able to induce a steady reduction in the number of progenitor cells, in contrast to control flies, which remained constant (Fig. 1A,B and Suppl. Fig. 1B). Jhamt is an O-methyl transferase that converts inactive precursors of JH to active JHs at the very last steps of JH biosynthesis in Drosophila.

Figure 1

Jhamt activity is required for the maintenance of intestinal homeostasis during aging. (A) jhamt RNAi (line 103237) expression in progenitor cells by esg-Gal4 (marked by UAS-GFP expression in green) shows a steady reduction in their number during a four week period at permissive temperature. Images show the same region of the anterior midgut in each condition. Similar results were obtained with the jhamt RNAi line 19172 (Suppl. Fig. 1B). (B) Histogram of the mean number of progenitor cells per total gut area. (C–E) jhamt RNAi expression on ISCs by Dl-Gal4 (C) and EBs by Su(H)GBE-Gal4 (D) reduce the number of ISCs and EBs respectively, marked by UAS-GFP expression (green). In contrast, jhamt RNAi expression in EEs by pros-Gal4 does not reduce their number (E). Histograms show the mean number of ISCs (C), EBs (D) and EEs (E) after two weeks at permissive temperature. Statistical analysis by Wilcoxon Ranked Sum Test: ****p < 0.0001; ***p < 0.001; **p < 0.01; NS, not significant. Error bars show standard deviation. At least 8 guts were analyzed in each condition.

jhamt RNAi expression under the DeltaGal4 (Dl-Gal4) driver line, which is expressed in the ISCs23 was able to significantly reduce the number of ISCs (Fig. 1C and Suppl. Fig. 1A). Similarly, jhamt RNAi expression under the EB-specific Su(H)GBE-Gal4 driver line23 was able to reduce the number of EBs (Fig. 1D and Suppl. Fig. 1A). On the contrary, jhamt RNAi expression under the pros-Gal4 driver line, expressed in differentiated EEs, did not significantly affect their numbers (Fig. 1E and Suppl. Fig. 1A). Together, this data uncovers a requirement of jhamt expression for the survival of ISCs and EBs in the aging adult gut.

Adult gut progenitor cells produce and respond to the JH activity

In insects, JHs are primarily synthesized and secreted by the CA to perform a systemic action3. However, the phenotype observed upon jhamt RNAi expression in ISCs and EBs suggested that the adult gut could act as local source of JH biosynthesis or conversion. Two lines of evidence support this notion. First, we observed that expression of RNAi lines against three other key enzymes required for JH biosynthesis, Farnesyl pyrophosphate synthase (FPPS), the farnesol dehydrogenase Sniffer (Sni) and the JH epoxidase Cyp305a124, 25, under the control of the Esg-Gal4 driver were also able to greatly reduce the number of progenitor cells (Fig. 2A,C and Suppl. Fig. 1B). Second, over-expression of Allatostatin C (Ast-C), a neuropeptide that inhibits JH biosynthesis26, 27 and is expressed in the nervous system and in EE cells along the gut28, 29 also reduced the number of intestinal progenitor cells (Fig. 2A,C).

Figure 2

JH is synthesized in the adult gut, which responds to it in an autocrine loop. (A) expression in progenitor cells by esg-Gal4 (marked by UAS-GFP expression in green) of RNAi transgenes against the JH biosynthetic enzymes Fpps (line 104362), sni (line 106219) and Cyp305a1 (line 101644), over-expression of Ast-C, a known inhibitor of JH biosynthesis, or RNAi mediated knock down of the JH nuclear receptors gce (lines 61852 and 26323 together) and Met (line 61935), as well as transcriptional coactivator tai (line 36095), all lead to reduced number of progenitor cells after two weeks at permissive temperature. Images show the same region of the anterior midgut in each condition. (B) Ablation of the CA does not reduce the number of progenitor cells, marked by the expression of 10xSTAT92E-GFP (in green). (C,D) Histogram of the mean number of progenitor cells per total gut area. (E) Two weeks old flies expressing jhamt RNAi in progenitor cells survive poorly while fed with 3% DSS compared to control flies. 40 control and 30 jhamt RNAi expressing flies were analyzed. Statistical analysis by Wilcoxon Ranked Sum Test: ***p < 0.001; **p < 0.01; NS, not significant. Error bars show standard deviation. At least 5 guts were analyzed in each condition.

Remarkably, this local gut-specific JH activity seemed to have an autocrine effect on the progenitor cell population, as the expression of the RNAi lines against the JH receptors Met, its paralog gce, and the steroid receptor coactivator Tai strongly reduced the number of progenitor cells (Fig. 2A,C and Suppl. Fig. 1B). Noticeably, however, the reduction in the number of progenitor cells was significantly stronger in flies expressing gce RNAi or tai RNAi than in flies expressing jhamt RNAi (Fig. 2A,C), indicating that the effect of JH activity on the survival of the progenitor cell population could depend on both local and systemic JH sources. In order to confirm that the local gut source of JH was able to maintain ISCs and EBs, we eliminated the effect of systemic JH by ablating the CA. Interestingly, the number of ISCs and EBs did not change significantly upon CA ablation (Fig. 2B,D). Together, these results show that the adult Drosophila gut is both the source and the target of a local JH activity, which is required to maintain the viability of the aging progenitor cells.

Physiological relevance of local JH in damage response

We next investigated whether the reduction in the number of progenitor cells produced by the reduction in the local JH synthesis had consequences in coping with oxidative insults, as a measurement of cellular fitness. Flies were fed a 5% sucrose diet supplemented with dextran sulfate sodium (DSS) in order to injure the gut epithelium. As expected, flies expressing jhamt RNAi under the esg-Gal4 control in progenitor cells showed a reduced life span compared to control flies (Fig. 2E), showing that the reduction in the number of progenitor cells affected gut homeostasis and its ability to cope with stress-induced damage.

Local JH regulates cell size

We noticed that upon jhamt RNAi expression under the esg-gal4 driver, progenitor cells became smaller (Fig. 3A). To better characterize the effect of the local JH activity on cellular size, we took advantage of the EB specific marker Su(H)-mCherry15. We sorted the ISC (GFP+, mCherry) and EB (GFP+, mCherry+) cell populations and confirmed that upon jhamt RNAi expression cells were consistently smaller on average (Fig. 3B). On the contrary, expression of an RNAi line against JH esterase (JHE), an enzyme required for the degradation of JH30, slightly increased the cellular size of ISCs and EBs (Fig. 3B), implying that local JH is directly involved in cell size regulation. Next we asked whether JH function on cell growth was dependent on the insulin/target or rapamycin (IIS/TOR) pathway, which links nutrient sensing to cellular growth31, 32. To activate the pathway we over expressed PI3K, the TOR activator Ras homolog enriched in brain (Rheb) and S6KII, or an RNAi against the negative pathway regulator PTEN, but none of them were able to restore normal progenitor cell size (Fig. 3C) or to increase their survival (Suppl. Fig. 2A) when expressed together with jhamt RNAi. We concluded, therefore, that the effect on cell growth mediated by local JH activity is independent or downstream to the IIS/TOR pathway.

Figure 3

Local JH regulates cell size and cell death. (A) Confocal images taken at 63x showing that expression of jhamt RNAi in ISCs (by Dl-Gal4) or EBs (by Su(H)GBE-Gal4) reduces cell size of ISCs and EBs respectively, compared to control flies. Cells are marked in green by UAS-GFP (in ISCs) and UAS-CD8-GFP (in EBs). (B) Contour plot from sorted cells show that ISCs and EBs expressing jhamt RNAi (in dark blue) are on average smaller than control cells (in red). On contrary, cells expressing jhe RNAi cells (light blue) are slightly bigger. Forward scatter area (FSC-A) on the x-axis and side scatter area (SSC-A) on the y-axis together outline the cell shapes. (C) Histogram of the average cell size shows that normal cell size is not restored by IIS/TOR pathway activation in cells expressing jhamt RNAi. (D,E) Tubulin > Gal4 driven MARCM clones expressing jhamt RNAi progressively disappear from the midgut compared to control clones (D) but the jhamt RNAi-expressing clones that survive show the same size distribution as control clones (E). (F) control and jhamt RNAi expressing clones (marked by UAS-GFP in green) are able to differentiate, showing ECs (marked by big polyploid nuclei stained by DAPI in blue) and EEs (marked by Prospero staining in red). (G) DNA content analysis of sorted jhamt RNAi expressing progenitor cells shows a G0 sub phase characteristic of apoptotic cells. (H) Histogram of the number of GFP+ cells/area shows that co-expression of the antiapoptotic UAS-Diap1 does not prevent cell death induced by jhamt RNAi expression. Statistical analysis by Wilcoxon Ranked Sum Test: ***p < 0.001; **p < 0.01; NS, not significant. Error bars show standard deviation. At least 5 guts were analyzed in each condition.

In some stem cell populations, as for example most neuroblasts of the central nervous system of Drosophila, a reduction in the cellular size precedes a terminal symmetric division during pupal development33. We analyzed whether the reduction in the number of progenitor cells upon jhamt RNAi expression was due to the reduction in cell size imposed by lack of JH activity, and a subsequent symmetric division resulting in two EBs. In order to test this possibility, we performed clonal analysis by generating MARCM clones34 expressing jhamt RNAi and GFP under the control of the ubiquitous tubulin promoter. We observed a reduction in the number of clones in flies expressing jhamt RNAi compared to control flies (Fig. 3D). However, surviving jhamt RNAi and wild type clones showed similar size distribution at one, two and four weeks after clone induction (Fig. 3E and Suppl. Fig. 2B). These results rule out the possibility that lack of JH activity induces ISCs symmetric division. Remarkably, this data indicates that ISCs surviving jhamt RNAi expression proliferate at the same rate than control ISCs. Moreover, we also observed that clones expressing jhamt RNAi were able to differentiate, as they contained ECs, detected by their large, polyploid nuclei and EEs, detected by Prospero (Pros) staining (Fig. 3F). Therefore, reduction of jhamt expression does not seem to impair the potential proliferation or differentiation of the cells that survive.

We next analyzed the DNA content of ISCs and EBs upon jhamt RNAi expression. As previously reported35, control flies showed ISCs mostly in G1 phase, with very few events in G2 phase (Fig. 3G). Interestingly, jhamt RNAi expression induced a sub-G0 phase in most ISCs, indicating DNA fragmentation and consequent loss of DNA. This observation suggests that in the absence of JH activity, ISCs may undergo an active process of cell death, consistent with the number reduction previously observed. Overexpression of Drosophila inhibitor of apoptosis 1 (Diap-1) in jhamt RNAi cells, however, did not restore the wild type number of progenitor cells (Fig. 3H), suggesting that cell death induced by lack of JH must be caspase-independent.

JH activity is required for tumor growth in the adult anterior midgut

We have previously described that mitotic clones mutant for Apc and Apc2 that co-express the oncogenic form of Ras, UAS-ras V12 under the control of esg-Gal4 driver line (from now on denoted as Apc-Ras clones), develop as tumor overgrowths in the Drosophila intestine. These tumor clones recapitulate several characteristics of human colorectal cancer, serving as a model that has already allowed the identification of previously unknown regulators of intestinal tumorigenesis (Fig. 4A)15, 36.

Figure 4

JH activity is required for Apc-Ras induced tumor growth. (A) Co-expression of jhamt RNAi in Apc-Ras clones reduces its size after four weeks of clone induction. Treatment with the JH analog methoprene (Met) rescues the growth of Apc-Ras clones. (B) RNAi expression of genes of the JH biosynthetic pathway (Fpps, sni and Cyp305a1) reduce the growth of Apc-Ras clones. (C) JH receptor gce RNAi expression, as well as tai RNAI expression block the growth of Apc-Ras clones. In contrast, RNAi against the JH receptor Met does not block Apc-Ras clone growth. (D) Histogram showing the GFP+ area/anterior midgut of the genotypes shown in a-c. Statistical analysis by Wilcoxon Ranked Sum Test: ***p < 0.001; **p < 0.01; NS, not significant. Error bars show standard deviation. At least 6 guts were analyzed in each condition.

Expression of jhamt RNAi in Apc-Ras clones not only dramatically reduced clone size at four weeks after clone induction (Fig. 4A,D) but also restored life span, which is reduced in flies bearing Apc-Ras clones alone (Suppl. Fig. 3A)15. Consistently, administration of the JH analog methoprene restored clone growth (Fig. 4A,D), confirming the role of JH activity in tumor growth. Accordingly, expression of RNAi against the JH biosynthetic enzymes Fpps, sni and Cyp305a1 or over-expression of Ast-C also reduced the size of Apc-Ras clones (Fig. 4B,D and Suppl. Fig. 3B).

Downregulation of the JH receptor gce or the coactivator tai also reduced the growth of Apc-Ras clones (Fig. 4C,D), showing that they directly respond to JH activity. Interestingly, expression of the RNAi line against Met was not able to impair tumor growth (Fig. 4C,D), suggesting that in a tumor context Met may not be involved in transducing the JH signal or that its function may be performed by its paralog gce. Taken together, these results suggest that, in Drosophila, gut JH activity is required to sustain tumor growth in an autocrine fashion.


JHs regulate many processes during larval development and adult insects. In the adult JHs play many roles, mostly related to reproduction, such as oogenesis, adult male courtship, female sex pheromone production or female gut remodeling in preparation for an increased energy expenditure in egg formation after mating1, 2, 5, 37, 38. It is widely accepted that JH is synthesized by the CA, from where it is secreted and performs a systemic action. In this work we identify a previously undescribed gut-specific source of JH biosynthesis, which acts in an autocrine fashion to maintain the survival of progenitor cells in the adult midgut of Drosophila during aging. To our knowledge this would be the first time JH is reported to be produced in an adult Drosophila organ other than the CA. In the mosquito Aedes aegypti, ovaries and male accessory glands synthesize JH39, 40, and it has been recently shown that male accessory glands transfer JH to females at mating41. Our claim that the adult gut produces an active JH is supported by several lines of evidence. First, RNAi mediated knock down of four genes that codify the enzymes required for JH biosynthesis specifically in midgut progenitor cells show a reduction in the survival of progenitor cells. Second, the same phenotype is observed upon over-expression of the JH biosynthesis inhibitor Ast-C. Third, ablation of adult CA, thus eliminating systemic JH does not affect the number of gut progenitor cells. Finally, growth of Apc-Ras clones expressing jhamt RNAi is rescued by treatment with the JH analog methoprene. However, further work is required to identify specifically which JH activity is being produced by the adult gut.

We report that this adult, gut-specific JH activity acts as a survival factor. JH works by promoting cell growth and suppressing a cell death program and not by regulating cell proliferation or cell differentiation. Cell growth is usually regulated by the IIS/TOR pathway, the components of which are not only essential for cell and organ growth but are also sufficient to accelerate cell growth rate31. In fact, activation of the IIS/TOR signaling pathway bypass the cellular effects of starvation such as the arrest of cell growth and DNA replication31, 42, 43. JH has been recently shown to regulate the final body size in an IIS/TOR dependent manner44, congruent with the notion that IIS signaling may regulate JH synthesis45. Our results show that expression of IIS/TOR components are not enough to restore the growth nor the survival of intestinal progenitor cells in absence of the local production of JH activity. Interestingly, the amount of local JH would directly regulate cell size, as over-expression of the RNAi against JHE is enough to increase the average cell size.

Our results also show that cells surviving the lack of JH activity are able to proliferate and differentiate normally. Why do these cells survive? One possibility is that it is a stochastic event related to a variable expression of RNAi transgenes. Another possibility could be that a subtype of progenitor cells evenly distributed along the gut does not require JH to maintain their fitness. However, the progressive phenotype that we observe seems to indicate that eventually all progenitor cells are sensitive to the lack of local JH activity. Overall, our results support the idea that the conjunction of both local and systemic JH activity modulates gut homeostasis. Local gut JH activity in progenitor cells would maintain a competent state to sustain normal functions, regulating its survival during aging. Loss of local JH activity would reduce the fitness of ISCs and EBs over time, reducing their size and eventually triggering a caspase-independent cell death program. Systemic JH would respond to external outputs such as mating5, and modify the capacity of the progenitor cells to proliferate, grow, or differentiate, adapting to external cues. Taking into consideration the role of JH preventing metamorphosis during larval development, a picture could emerge from our results in which JH would maintain the progenitor cells in a “juvenile” state in a mostly post-mitotic organism.

Finally, our results also show that local JH is required for intestinal tumor growth in Drosophila. Moreover, methoprene treatment is able to sustain growth in the absence of local JH production. These results open the possibility that external cues that increase the systemic level of JH could eventually incise in the rate of growth of tumor cells. In addition, considering the functional homology between JH and retinoic acid, it needs to be investigated in further detail whether hormones produced locally may play a role sustaining tumor growth in colorectal cancer patients.

Materials and Methods

Fly stocks

The lines of jhamt RNAi (103237 and 19172), Fpps RNAi (104362), Sni RNAi (106219 and 27342), Cyp305a1 RNAi (101644 and 51486), Met RNAi (100638 and 10801), gce RNAi (11176), tai RNAi (15709), Jhe RNAi (44049) and PTEN RNAi (109278) were obtained from VDRC. Met RNAi (61935), gce RNAi (combination of 61852 and 26323 at the same time), tai RNAi (36095), UAS-PI3K (8287), UAS-Rheb (9689), UAS-S6KII (8714), 10xSTAT92E-GFP (26197), UAS-EGFP-NiPp1 (23712) and Aug21Gal4 (30137) were obtained from Bloomington Stock Center. UAS Diap-1 was a gift from G. Morata. UAS-AstC was generated cloning the EcoRI-KpnI fragment from the cDNA clone RH36507 (DGC gold BDGP) into the pUAST vector. Transgene expression together with UAS-GFP was driven by esg-Gal4, Dl-Gal4, Su(H)-Gal4 or pros-Gal4. Gal4 activity was regulated by Tub > Gal80 ts. Flies were crossed at 17 °C, and two day old progeny was transferred to 29 °C for analysis. MARCM clones were generated by a 1hr heat shock at 37 °C of 2–5 days old females and were marked by the tubulin > Gal4 line driving the expression of UAS GFP (normal clones). Apc-Ras clones were generated as described previously15.

Immunohistochemistry and microscopy

Adult female flies were dissected in PBS. All the digestive tract was removed and fixed in PBS and 4% electron microscopy grade paraformaldehyde (Polysciences, USA) for 40 minutes. Samples were rinsed 3 times with PBS, 4% BSA, 0.1% Triton X-100 (PBT-BSA), incubated with the primary antibody overnight at 4 °C and with the secondary antibody for 2 hours at room temperature. Finally, the samples were rinsed 3 times with PBT-BSA and mounted in DAPI-containing media (Vectashield, USA). All the steps were performed without mechanical agitation. Primary antibody mouse α-Pros (1:100) was obtained from the Developmental Studies Hybridoma Bank (DSHB). Secondary antibodies were from Invitrogen (USA). Images were obtained on a Leica SPE or Leica SP5 confocal microscopy and processed in Photoshop CS5 (Adobe, USA).

Corpus allatum ablation

Abolishment of endogenous adult JH production by ablation of the CA was performed by misexpression of the protein phosphatase inhibitor NiPp1 under the CA-specific driver Aug21-Gal4 46, 47 in a genetic background that contained the ISC and EB marker 10xSTAT92E-GFP 48.

FACS analysis

20–30 female fly guts were dissected and collected in cold PBS and kept on ice. Guts were digested with 40 mg/ml of Dispase (Invitrogen) for 5 minutes at 37 °C. The guts were then passed through a hypodermic needle using 1 ml syringe repeatedly until the PBS looks slightly cloudy. Cells were checked under microscope to ascertain they were single celled and without clumps and then washed with cold PBS by spinning @1400 rpm/5 minutes/4 °C and re-suspended with cold PBS to a final volume of 200 µl and transferred into a “flow tube” for further nuclear staining and FACS analysis. Cells were sorted with FACS Aria from BD Biosciences and the results were analyzed with Flow Jo V10.0.8 from Tree Star, Inc. Oregon.

Stress experiments

Flies of appropriate age and genotype were transferred to empty vials and fed with 5% sucrose with or without 3% DSS. The number of dead flies was scored at different time points after the introduction of the DSS.


  1. 1.

    Nijhout, H. F. Insect hormones. (Princeton University Press, Princeton, 1994).

  2. 2.

    Flatt, T., Tu, M. P. & Tatar, M. Hormonal pleiotropy and the juvenile hormone regulation of Drosophila development and life history. BioEssays: news and reviews in molecular, cellular and developmental biology 27, 999 (2005).

    CAS  Article  Google Scholar 

  3. 3.

    Jindra, M., Palli, S. R. & Riddiford, L. M. The juvenile hormone signaling pathway in insect development. Annual review of entomology 58, 181 (2013).

    CAS  Article  PubMed  Google Scholar 

  4. 4.

    Yamamoto, R. B., Dolezal, H., Amdam, A.G., Tatar, G.M. Juvenile Hormone regulation of Drosophila aging. BMC Biology 11:85 (2013).

  5. 5.

    Reiff, T. et al. Endocrine remodelling of the adult intestine sustains reproduction in Drosophila. eLife 4, e06930 (2015).

    Article  PubMed  PubMed Central  Google Scholar 

  6. 6.

    Micchelli, C. A. & Perrimon, N. Evidence that stem cells reside in the adult Drosophila midgut epithelium. Nature 439, 475 (2006).

    ADS  CAS  Article  PubMed  Google Scholar 

  7. 7.

    Ohlstein, B. & Spradling, A. The adult Drosophila posterior midgut is maintained by pluripotent stem cells. Nature 439, 470 (2006).

    ADS  CAS  Article  PubMed  Google Scholar 

  8. 8.

    Zeng, X. & Hou, S. X. Enteroendocrine cells are generated from stem cells through a distinct progenitor in the adult Drosophila posterior midgut. Development 142, 644 (2015).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Biteau, B. & Jasper, H. Slit/Robo signaling regulates cell fate decisions in the intestinal stem cell lineage of Drosophila. Cell reports 7, 1867 (2014).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  10. 10.

    Jiang, H., Tian, A. & Jiang, J. Intestinal stem cell response to injury: lessons from Drosophila. Cellular and molecular life sciences: CMLS 73, 3337 (2016).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  11. 11.

    Pasco, M. Y. L. & Gallet A., R. The cellular homeostasis of the gut:what the Drosophila model points out. Histol Histopathol 30, 277 (2015).

    CAS  PubMed  Google Scholar 

  12. 12.

    Lemaitre, B. & Miguel-Aliaga, I. The digestive tract of Drosophila melanogaster. Annual review of genetics 47, 377 (2013).

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Jiang, H. & Edgar, B. A. Intestinal stem cells in the adult Drosophila midgut. Experimental cell research 317, 2780 (2011).

    CAS  Article  PubMed  Google Scholar 

  14. 14.

    Guo, Z., Ohlstein, B. Stem cell regulation. Bidirectional Notch signaling regulates Drosophila intestinal stem cell multipotency. Science 350 (Nov 20, 2015).

  15. 15.

    Martorell, O. et al. Conserved mechanisms of tumorigenesis in the Drosophila adult midgut. PloS one 9, e88413 (2014).

    ADS  Article  PubMed  PubMed Central  Google Scholar 

  16. 16.

    Wang, C. et al. APC loss-induced intestinal tumorigenesis in Drosophila: Roles of Ras in Wnt signaling activation and tumor progression. Developmental biology 378, 122 (2013).

    CAS  Article  PubMed  Google Scholar 

  17. 17.

    Rivera-Perez, C., Nouzova, M., Lamboglia, I. & Noriega, F. G. Metabolic analysis reveals changes in the mevalonate and juvenile hormone synthesis pathways linked to the mosquito reproductive physiology. Insect biochemistry and molecular biology 51, 1 (2014).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  18. 18.

    Shinoda, T. & Itoyama, K. Juvenile hormone acid methyltransferase: a key regulatory enzyme for insect metamorphosis. Proceedings of the National Academy of Sciences of the United States of America 100, 11986 (2003).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  19. 19.

    Niwa, R. et al. Juvenile hormone acid O-methyltransferase in Drosophila melanogaster. Insect biochemistry and molecular biology 38, 714 (2008).

    CAS  Article  PubMed  Google Scholar 

  20. 20.

    Gilbert, L. I., Granger, N. A. & Roe, R. M. The juvenile hormones: historical facts and speculations on future research directions. Insect Biochem. Mol. Bio. 30, 617 (2000).

    CAS  Article  Google Scholar 

  21. 21.

    Jindra, M., Uhlirova, M., Charles, J. P., Smykal, V. & Hill, R. J. Genetic Evidence for Function of the bHLH-PAS Protein Gce/Met As a Juvenile Hormone Receptor. PLoS genetics 11, e1005394 (2015).

    Article  PubMed  PubMed Central  Google Scholar 

  22. 22.

    Bai, J., Uehara, Y. & Montell, D. J. Regulation of invasive cell behavior by taiman, a Drosophila protein related to AIB1, a steroid receptor coactivator amplified in breast cancer. Cell 103, 1047 (2000).

    CAS  Article  PubMed  Google Scholar 

  23. 23.

    Zeng, X., Chauhan, C. & Hou, S. X. Characterization of midgut stem cell- and enteroblast-specific Gal4 lines in drosophila. Genesis 48, 607 (2010).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  24. 24.

    Belles, X., Martin, D. & Piulachs, M. D. The mevalonate pathway and the synthesis of juvenile hormone in insects. Annual review of entomology 50, 181 (2005).

    CAS  Article  PubMed  Google Scholar 

  25. 25.

    Huang, J. et al. DPP-mediated TGFbeta signaling regulates juvenile hormone biosynthesis by activating the expression of juvenile hormone acid methyltransferase. Development 138, 2283 (2011).

    CAS  Article  PubMed  Google Scholar 

  26. 26.

    Price Merte, D. P. et al. melanogaster flatline encodes a myotropin orthologue to Manduca sexta allatostatin. Peptides 23, 787 (2002).

    Article  PubMed  Google Scholar 

  27. 27.

    Wang, C., Zhang, J., Tobe, S. S. & Bendena, W. G. Defining the contribution of select neuropeptides and their receptors in regulating sesquiterpenoid biosynthesis by Drosophila melanogaster ring gland/corpus allatum through RNAi analysis. General and comparative endocrinology 176, 347 (2012).

    CAS  Article  PubMed  Google Scholar 

  28. 28.

    Stay, B. & Tobe, S. S. The role of allatostatins in juvenile hormone synthesis in insects and crustaceans. Annual review of entomology 52, 277 (2007).

    CAS  Article  PubMed  Google Scholar 

  29. 29.

    Beehler-Evans, R. & Micchelli, C. A. Generation of enteroendocrine cell diversity in midgut stem cell lineages. Development 142, 654 (2015).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  30. 30.

    Liu, Z., Li, X., Prasifka, J. R., Jurenka, R. & Bonning, B. C. Overexpression of Drosophila juvenile hormone esterase binding protein results in anti-JH effects and reduced pheromone abundance. General and comparative endocrinology 156, 164 (2008).

    CAS  Article  PubMed  Google Scholar 

  31. 31.

    Edgar, B. A. How flies get their size: genetics meets physiology. Nature reviews. Genetics 7, 907 (2006).

    CAS  Article  PubMed  Google Scholar 

  32. 32.

    Andersen, D. S., Colombani, J. & Leopold, P. Coordination of organ growth: principles and outstanding questions from the world of insects. Trends in cell biology 23, 336 (2013).

    CAS  Article  PubMed  Google Scholar 

  33. 33.

    Maurange, C., Cheng, L. & Gould, A. P. Temporal transcription factors and their targets schedule the end of neural proliferation in Drosophila. Cell 133, 891 (2008).

    CAS  Article  PubMed  Google Scholar 

  34. 34.

    Lee L., T. L. Mosaic Analysis with a Repressible Cell Marker for Studies of Gene Function in Neuronal Morphogenesis. Neuron 22, 451 (1999).

    Article  Google Scholar 

  35. 35.

    Amcheslavsky, A., Ito, N., Jiang, J. & Ip, Y. T. Tuberous sclerosis complex and Myc coordinate the growth and division of Drosophila intestinal stem cells. The Journal of cell biology 193, 695 (2011).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  36. 36.

    Martorell, O. et al. Iro/IRX transcription factors negatively regulate Dpp/TGF-beta pathway activity during intestinal tumorigenesis. EMBO reports 15, 1210 (2014).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  37. 37.

    Wijesekera, T. P., Saurabh, S. & Dauwalder, B. Juvenile Hormone Is Required in Adult Males for Drosophila Courtship. PloS one 11, e0151912 (2016).

    Article  PubMed  PubMed Central  Google Scholar 

  38. 38.

    Bilen, J., Atallah, J., Azanchi, R., Levine, J. D. & Riddiford, L. M. Regulation of onset of female mating and sex pheromone production by juvenile hormone in Drosophila melanogaster. Proceedings of the National Academy of Sciences of the United States of America 110, 18321 (2013).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  39. 39.

    Borovsky, D. C., Ujváry, D. A. & Prestwich, I. G.D. Biosynthesis of (10R)-Juvenile Hormone III From Farnesoic Acid by Aedes aegypti Ovary. Archives of Insect Biochemistry and Physiology 27, 11 (1994).

    CAS  Article  Google Scholar 

  40. 40.

    Borovsky, D., Carlson, D. A., Hancock, R. G., Rembold, H. & van Handel, E. De novo biosynthesis of juvenile hormone III and I by the accessory glands of the male mosquito. Insect biochemistry and molecular biology 24, 437 (1994).

    CAS  Article  PubMed  Google Scholar 

  41. 41.

    Clifton, M. E., Correa, S., Rivera-Perez, C., Nouzova, M. & Noriega, F. G. Male Aedes aegypti mosquitoes use JH III transferred during copulation to influence previtellogenic ovary physiology and affect the reproductive output of female mosquitoes. Journal of insect physiology 64, 40 (2014).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  42. 42.

    Britton, J. S., Lockwood, W. K., Li, L., Cohen, S. M. & Edgar, B. A. Drosophila’s insulin/PI3-kinase pathway coordinates cellular metabolism with nutritional conditions. Developmental cell 2, 239 (2002).

    CAS  Article  PubMed  Google Scholar 

  43. 43.

    Saucedo, L. J. et al. Rheb promotes cell growth as a component of the insulin/TOR signalling network. Nature cell biology 5, 566 (2003).

    CAS  Article  PubMed  Google Scholar 

  44. 44.

    Mirth, C. K. et al. Juvenile hormone regulates body size and perturbs insulin signaling in Drosophila. Proceedings of the National Academy of Sciences of the United States of America 111, 7018 (2014).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  45. 45.

    Tatar, M. et al. A mutant Drosophila insulin receptor homolog that extends life-span and impairs neuroendocrine function. Science 292, 107 (2001).

    ADS  CAS  Article  PubMed  Google Scholar 

  46. 46.

    Parker, L. et al. Functional interaction between nuclear inhibitor of protein phosphatase type 1 (NIPP1) and protein phosphatase type 1 (PP1) in Drosophila: consequences of over-expression of NIPP1 in flies and suppression by co-expression of PP1. The Biochemical journal 368, 789 (2002).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  47. 47.

    Yamamoto, R., Bai, H., Dolezal, A. G., Amdam, G. & Tatar, M. Juvenile hormone regulation of Drosophila aging. BMC Biol 11, 85 (2013).

    Article  PubMed  PubMed Central  Google Scholar 

  48. 48.

    Beebe, K., Lee, W. C. & Micchelli, C. A. JAK/STAT signaling coordinates stem cell proliferation and multilineage differentiation in the Drosophila intestinal stem cell lineage. Developmental biology 338, 28 (2010).

    CAS  Article  PubMed  Google Scholar 

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We are thankful to IRB Barcelona Cell separation Unit and to VDRC and Bloomington stock center for fly stocks. We thank Yolanda Rivera for technical support. We thank Jordi Casanova for comments on the manuscript. We are grateful to members of Jordi Casanova lab for constructive discussion. We thank Idun Dale Rein for FACS analysis. MMR was supported by the Marie Curie Cofund Fellowship under FP7 programme of the ERC. This work was supported by the MICINN (BFU2014-59781P) to AC and MICINN (CGL2014-55786P) and SGR-2014 from Catalan Government to XF-M and D. M. The research has also benefited from FEDER funds.

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M.M.R. designed and performed experiments, analyzed data and prepared the figures. X.F.M., J.L.M. and D.M. performed experiments and contributed to the conceptual development of the project and to data interpretation. A.C. conceived and designed the experiments, performed experiments, interpreted the data and wrote the manuscript.

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Correspondence to A. Casali.

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Rahman, M.M., Franch-Marro, X., Maestro, J.L. et al. Local Juvenile Hormone activity regulates gut homeostasis and tumor growth in adult Drosophila . Sci Rep 7, 11677 (2017). https://doi.org/10.1038/s41598-017-11199-9

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