Nap1 and Chz1 function in a coordinated manner to restrict the spread of heterochromatin. (a) Schematic of the telomeric and mating type locus on chromosome III showing the neighboring genes. (b) qRT-PCR analysis of the genes represented in the schematic in different mutant strains of indicated genotypes (materials and methods). SWR1 and HTZ1 deletions were used a positive control. One way ANOVA was used to measure the statistical significance.