Biochemical analysis of H2A.Z mutants predicted to destabilize Chz1-H2A.Z interaction. (a) Table showing the list of computationally predicted mutants (HTZ1 Mutation), the region they belong to in the nucleosome structure (Structural Region), and the effect of the mutation on the computationally calculated binding energy between H2A.Z-H2B and Chz1 (ΔΔG (Mean ± S.E.)). S.E. refers to standard error of mean. (b) Phenotypic analyses of H2A.Z mutants predicted to destabilize H2A.Z-Chz1 interaction. Shown is are 5-fold serial dilutions of the indicated strains that were spotted on either control (Sc-Ura) or drug-containing plates. (c) Immunoblots showing H2A.Z levels in whole cell extracts (top) and in chromatin (bottom) from wild-type and various H2A.Z mutant strains.