Molecular recognition and structure of H2A.Z-H2B by Chz1. (a) The six centroid structures from the refined DMD ensemble of H2A.Z-H2B-Chz1 complex are structurally aligned and displayed using cartoon representation. The centroid structures are overlaid over the yeast nucleosome structure in gray, highlighting the surface on H2A.Z-H2B that is bound by both Chz1 and DNA. (b) The H2A.Z-H2B dimer is displayed using surface representation with the residues colored according to the average number of interface contacts they form with Chz1, which is displayed with the cartoon representation. The structures are overlaid over the yeast nucleosome structure in gray. The dashed-box indicates the region of H2A.Z-H2B surface that forms the acidic patch. The asterisk indicates the DNA-binding surface of H2A.Z-H2B. (c) The acidic patch from the H2A.Z-H2B dimer is shown using surface representation forming interactions from three arginine residues from Chz1. (d) The DNA binding surface of H2A.Z-H2B is shown colored according to interface contacts. The nucleosome structure in gray is overlaid to highlight the path of DNA on this surface.