Table 1 Kinetic parameters comparison between Myo19-3IQ and MyoW140V-3IQ.

From: ROS induced distribution of mitochondria to filopodia by Myo19 depends on a class specific tryptophan in the motor domain

Parameter Value
Myo19-3IQ5 Myo19-3IQW140V
Steady-state ATPase parameters
k cat (s−1 head−1) 3.1 ± 0.1 4.1 ± 0.4
K ATPase (µM) 11.6 ± 1.2 56.7 ± 10.9
v o (s−1) 0.06 ± 0.02 0.10 ± 0.05
ATP binding
1/K1T (µM) 476 ± 116 758 ± 217
K+2T (s−1) 1003 ± 26 381 ± 72
K1T K+2T (μM−1s−1) 2.17 ± 0.8 0.5 ± 0.2
K−2T (s−1) ≈0 1.4 ± 0.3
k (s−1) 17.5 ± 0.3 29.3 ± 4.3
k-α (s−1) 2.6 ± 1.1 10.1 ± 6.0
K α 3 6.8 ± 1.6 2.9 ± 1.3
ADP binding
K′-1D (s−1) 9.3 ± 0.3 12.7 ± 1.5
1/KD,overall (µM) 0.13 ± 0.04 1.6 ± 0.3