Figure 9 | Scientific Reports

Figure 9

From: ROS induced distribution of mitochondria to filopodia by Myo19 depends on a class specific tryptophan in the motor domain

Figure 9

Simulation of the ATPase activity and duty ratio of Myo19-3IQ (WT) and Myo19W140V-3IQ (W140V). (a) Simulated steady state [actin]-dependence of the ATPase activity of the Myo19-3IQ and the mutant Myo19W140V-3IQ. The solid lines through the data points are the best fit to a rectangular hyperbola (\(v={v}_{0+}({k}_{cat}[actin])/({K}_{ATPase}+[actin])\)). (b) Duty ratio as a function of [actin]. The duty ratio was calculated from the summation of all the biochemical intermediates distribution at each [actin] at the steady-state time regime according to equation: duty ratio = (strongly bound states)/(strongly bound states + weakly bound states). The solid lines through the data points are the best fit to a rectangular hyperbola (\(duty\,ratio=1\ast [actin])/({K}_{DR}+[actin])\)). Data for Myo19-3IQ (WT) is reproduced from (Usaj & Henn, parallel submission).