Figure 5 | Scientific Reports

Figure 5

From: Transcriptome analysis of developing lens reveals abundance of novel transcripts and extensive splicing alterations

Figure 5

Functional analysis of the genes associated with high confident retained intron events across lens developmental stages. (a) Overview of intron retention mechanism (b) Functional enrichment analysis of genes associated with significant (FDR 1%) intron retention events identified using rMATS27 in atleast one pair of developmental stages compared. For each biological process per group (color coded), the % genes per GO term with number of query genes (** in red) in the analysis was shown in histogram. This shows the functional grouping of the GO-terms based on GO hierarchy was represented as Clustered GO-network using the Cytoscape67-ClueGO31 plugin. Significant clusters (p < 1e-2), color coded by group based on enriched GO-biological processes generated from ClueGO analysis with size of the nodes indicating level of significant association of genes per GO-term. (c) Bubble plot showing the alterations in the inclusion levels of a retained intron for Celf1 across various developmental stages. Each bubble shows the Percent Spliced Index (PSI) of the retained intron indicating an increase in the inclusion level from embryonic to post-natal stages.