SOX-2 positive endogenous stem cells were activated 2 weeks after SCI, while RAGE blockade reduced SOX-2/RAGE double-positive cells. (a) Figures with the blue fluorescence dye are for the nucleus. Figures with the green fluorescence stand for SOX-2-positive cells. Figures with red fluorescence stand for RAGE-positive cells. Figures merged with DAPI/SOX-2/RAGE showed double-positive cells. In the sham group, SOX-2 positive cells were seldom found and rarely co-expressed with RAGE. At 2 weeks after SCI, increased SOX-2/RAGE double-positive cells were observed, but the anti-RAGE treatment group reduced SOX-2 expression at 2 weeks after injury. Higher magnification shows the area of a white square with magnification of *2.5, and the white arrow showed positive expression cells. Scar bar was 100 µm. (b) Relative immunofluorescence intensity of SOX-2-positive cells. (c) Relative immunofluorescence intensity of RAGE-positive cells. (d) Percentage of SOX-2/RAGE double-positive cell (%).