RAGE blockade reduced number of Nestin-positive NSCs that co-expressed RAGE 3 days after SCI. (a) Figures with the blue fluorescence dye are for the nucleus. Figures with the green fluorescence stand for nestin-positive cells. Figures with red fluorescence stand for RAGE-positive cells. Figures merged with DAPI/NESTIN/RAGE showed double-positive cells. In the sham group, nestin-positive cells can seldom be found, while RAGE expression can be found in neuronal cells. In the SCI group, we found increased nestin-positive and RAGE-positive cells expression after SCI. In the anti-RAGE group, we observed less nestin expression and most of nestin-positive cells were co-expressed with RAGE. Higher magnification shows the area of a white square, which represents magnification of *2.5, and the white arrow shows positive expression cells. Scar bar was 100 µm. (b) Relative immunofluorescence intensity of Nestin-positive cells. (c) Relative immunofluorescence intensity of RAGE-positive cells. (d) Percentage of Nestin/RAGE double-positive cells %.