Figure 1 | Scientific Reports

Figure 1

From: Downregulation of PARP1 transcription by promoter-associated E2F4-RBL2-HDAC1-BRM complex contributes to repression of pluripotency stem cell factors in human monocytes

Figure 1

Downregulation of stem cell transcription factors is associated with PARP1 repression. The expression of PARP1, selected transcription factors as well as PU.1/SPI-1 was compared between human hematopoietic stem and progenitor cells (HSPC) and monocytes with real-time PCR (b,d–j,l) and, additionally, PARP1 and PU.1 protein was determined using western blot (a and k). The accumulation of nascent mRNA (c) in HPSC and monocytes was monitored using Click-iT® Nascent RNA Capture Kit (Thermofisher Scientific).

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