Visual depiction of sequence alignment to reference genome, VP2 gene location within the canine parvovirus genome and analysis of 18 amino acid positions. Parvo6 and Parvo9 were chosen as representatives of each group of viruses to visualize NGS data and varying sequencing depths for each major protein. The VP2 region of each specimen was analyzed at key amino acid positions reflecting canine parvovirus subtype. Parvovirus specimens are ordered and categorized as they are depicted in Fig. 1 (phylogram). “New” canine parvovirus 2a and 2b stem from emerging viruses showing variability at the 426 aa position27.