Figure 1 | Scientific Reports

Figure 1

From: A Plasmodium plasma membrane reporter reveals membrane dynamics by live-cell microscopy

Figure 1

Generation and immunofluorescence analysis of PbPMP1-GFP parasites. (a) Schematic representation of the pL0017CPbPMP1-GFP-CmCherry plasmid. The PbPMP1-GFP fusion protein and a cytosolic mCherry were both expressed under the control of the constitutive eef1α promoter. The 3′-UTR was taken from Pbdhfr/ts. (b) PbPMP1-GFP localizes to the PPM in liver stage parasites. HeLa cells were infected with PbPMP1-GFP parasites and fixed at 54 hours post-infection (hpi). IFA was performed using antisera against the PPM marker MSP1 and the PVM marker EXP1. The PbPMP1-GFP signal was enhanced by staining with an anti-GFP antiserum (green). MSP1/EXP1 (purple). Parasite cytosol (red). The merged channels additionally contain DAPI-stained nuclei (blue). Scale bars = 10 µm.