Effect of different treatments (LPS, LPS + YW3-56, or HS) on blood levels of CitH3 in mouse models of LPSS and HS. Mice were intraperitoneally injected with LPS (35 mg/kg) in presence or absence of YW3-56 (A) or received hemorrhage (30% blood loss) (C). Animals from Sham group received all procedures except cannulation and hemorrhage. Blood was harvested at 0.5, 3, 12 and 24 hours after treatments. Circulating CitH3 was detected by Western blotting. Ponceau S stain was performed as loading control. Densitometry units were measured by Image J (B and D). Serum from LPS treated mice (35 mg/kg, 12 hours post LPS) served as positive control (CTL) (C). Data are shown as mean ± SD, n = 3/group. CitH3: Citrullinated histone H3; LPS: Lipopolysaccharide; HS: Hemorrhagic shock; DMSO: dimethyl sulfoxide.