Table 2 Genotyping of spontaneous mutations, sgRNA/Cas9 targeted modifications and phenotypes of five targeted lambs.

From: Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9

Lambs Targeted Modifications Frame Shift/In Frame Premature Stop Native Mutation Phenotype
GM081 −4bp(TATC) 3 N + 1 N = 1 trunkated N53 Aa D9N9N5N5 badger face with black body coat
−2bp(AT) 3 N + 2 N = 0 trunkated N64 Aa
GM106 −4bp(TATC) 3 N + 1 N = 1 trunkated N53Aa N9N9D5N5 badger face with black body coat and white dot on the hinder back
−6bp(TCTATC) 3 N N = 2 deletion /
+1 bp(A) 3 N + 1 N = 0 trunkated N65Aa
GM110 −4bp(TATC) 3 N + 1 N = 1 trunkated N53 Aa N9N9N5N5 Brown with badgerface and white color on the hinder tail and neck botom
−27bp(TGG-AGTA) 3 N N = 9 deletion /
GM109 −2bp(AT) 3 N + 2 N = 0 trunkated N64 Aa N9N9D5N5 Black/Brown-White dot color(with white on the head, leg and neck)
−4bp(TATC) 3 N + 1 N = 1 trunkated N53 Aa
+3 bp(TTG) 3 N N = 1 deletion /
GM105 −2bp(TC) 3 N + 2 N = 0 trunkated N64 Aa N9N9D5N5 Black and white dotted
+1 bp(A) 3 N + 1 N = 0 trunkated N65 Aa
WT108 / / / / Wild type D9N9N5N5 white
  1. Footnotes: N, denotes the number of triplet code; 3 N, denotes “in frame”; 3 N + 1 and 3 N + 2 denote “frameshift”.