Table 1 Summary on generation of CRISPR/Cas9 targeted lambs by injection of sgRNA/Cas9 mRNA into cytoplasm of zygotes.

From: Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9

Donor Ewe Collected Embryos Injected Embryo Cleavage Ratio (%) Embryos For ET Surrogate Ewes Conception Rate (%) (Pregnancies /Surrogates) Lambs Born Gene Modified Lambs GM Ratio (%) (GM Lambs/ Lambs Born)
14 113 105 87.62 (92/105) 92 60 10 (6/60) 6 5 83.33 (5/6)