Figure 5 | Scientific Reports

Figure 5

From: Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9

Figure 5

Varieties of targeted modifications of five targeted lambs. The left listed the targeted and wild type lambs. Each type of modifications was illustrated in the sequence in right of each lamb, which was determined by T-A cloning sequencing. The sgRNA sequence was marked in red and PAM was in green. The targeted modifications and ratios of colonies with modifications in the total colonies were displayed in the right. At least 19 T-A cloning colonies derived from the PCR products of each targeted lamb were sequenced. “−” represented deletion, “+” or “” represented insertion. N/N indicated the number of sequences with respective modification to the total sequencing colonies.

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