The dodo, Raphus cucullatus, a flightless pigeon endemic to Mauritius, became extinct during the 17th century due to anthropogenic activities. Although it was contemporaneous with humans for almost a century, little was recorded about its ecology. Here we present new aspects of the life history of the dodo based on our analysis of its bone histology. We propose that the dodo bred around August and that the rapid growth of the chicks enabled them to reach a robust size before the austral summer or cyclone season. Histological evidence of molting suggests that after summer had passed, molt began in the adults that had just bred; the timing of molt derived from bone histology is also corroborated by historical descriptions of the dodo by mariners. This research represents the only bone histology analysis of the dodo and provides an unprecedented insight into the life history of this iconic bird.
The dodo (Raphus cucullatus), a giant flightless pigeon, was endemic to Mauritius1, one of the Mascarene Islands in the southwestern Indian Ocean. It was first described in 1598 by Dutch mariners2, and became extinct less than a hundred years later (between 1662 and 1693)3, 4. It has now become an icon of extinction and a classic symbol of a human-induced extinction event. Although over hunting has been cited as a cause of the dodo’s demise, the introduction of invasive mammals, especially monkeys, deer, pigs and rats were primarily responsible3.
The skeletal anatomy of the dodo has been well studied5, and more recently Claessens et al.6 used novel methods to further understand its anatomy. Taxonomic studies have identified the dodo as a member of Columbiformes, and as the sister taxon to the Solitaire (Pezophaps solitaria)1, 7, but the ecology of the dodo has remained difficult to determine. The latter is perhaps explained by the fact that living Columbiformes differ considerably from the dodo making ecological comparisons difficult, and perhaps more significantly, the dodo became extinct long before any detailed ecological studies were carried out on Mauritius. Although some direct observations of the dodo are available from the 16th and 17th century, these are by sailors with no scientific background and are mainly contradictory and imprecise8. A number of contemporary illustrations of the dodo are known, but most are considered unreliable8. Recent body mass estimates range from 9.5 kg to 14.3 kg9,10,11,12; but almost no other information exists. Our research is therefore highly significant in that using bone microstructure we provide novel insight into the reproductive behavior, growth strategy, and molting habits of this recently extinct enigmatic bird13.
The histological structure of the bones from the Mare aux Songes (MAS) locality is generally much better preserved than subfossils recovered from the cave environments (Supplementary Table S1).
The histology of the dodo is similar to that of modern birds, which generally have three distinct layers that make up the bone wall12, 13. The central layer typically consists of fibrolamellar bone, which is a rapidly-deposited woven tissue, rich in primary osteons. This is overlain by a poorly vascularized or avascular tissue termed an outer circumferential layer (OCL), and underlain by an inner circumferential layer (ICL), which is composed of more slowly deposited lamellar bone tissue (terminology sensu Chinsamy-Turan13; Fig. 1c–f, Supplementary Table S2).
Our sample of dodo bones shows different stages of growth and maturity. Tibiotarsus (ddtbt14), has a cortex largely comprising of fibrolamellar bone tissue consisting of woven bone with many primary osteons, which suggests a rapid rate of bone deposition. Remnants of the periosteum along the outer surface are preserved and an inner circumferential layer (ICL), without resorption traces, is well developed (Fig. 1a,b, Supplementary Table S2). These characteristics indicate a late juvenile stage that has passed the most rapid phase of growth, but one that has not yet reached adulthood13, 14, making it the youngest specimen in our sample; all the other specimens studied appear to be adults, at slightly different stages of maturity.
Several specimens appear to be young adults in their first year post sexual maturity (ddfem02, ddfem03, ddfem04, ddfem05, ddhu01, ddtbt04). These individuals have a single line of arrested of growth (LAG) and they show the beginning of the development of the OCL [representing about 5% to 20% of the bone wall thickness (BWT; Figs 2d,e, 3 and 4d,e; Supplementary Table S2)], which indicates their skeleton has reached maturity15,16,17,18,19. These specimens all show some evidence of endosteal resorption, and they have a well-developed ICL, which comprises 15% to 35% of the cortex thickness (Supplementary Table S2). The rest of the sample (ddfem01, ddtbt01, ddtbt02, ddtbt03, ddtbt05, ddtbt06, ddtbt08, ddtbt09, ddtbt10, ddtbt13) represent more mature adults. They have a well-developed OCL of variable thickness, between 7% to 29% of the BWT (Supplementary Table S2), with two to five/six LAGs (Figs 1c–f, 2a,b, 4a,b and 5). The ICL in these specimens is generally thick, making up 14% to 51% of the BWT (Supplementary Table S2), and is often affected by endosteal resorption (Figs 1c–f, 2a,b, 4 and 5).
Only the tarsometatarsi (ddtmt01 and ddtmt02) show a large amount of secondary reconstruction, which obscures the triple-layer structure of the bone wall. This could be linked to biomechanical adaptations13, or possibly suggests the importance of these bones as calcium reservoirs20.
Identification of females
For two of the specimens, one tibiotarsus (ddtbt08) and one femur (ddfem04), a distinctive woven bone tissue (medullary bone)13, extends centripetally from the ICL into the medullary cavity (Fig. 2; Supplementary Table S3). This bone tissue develops in ovulating birds and is utilized in the formation of the calcium rich eggshells3, 19. The tibiotarsus (ddtbt08) has a large amount of medullary bone, making up about 4.5% of the BWT (Supplementary Table S3), while the femur (ddfem4) preserves medullary bone only in some areas of the bone wall, being approximately 1.5% of the BWT (Supplementary Table S3).
Osteohistological indicator of molting
Most adult specimens have many secondary osteons or resorption cavities in the compacta. One femur (ddfem03) and four tibiotarsi (ddtbt02, ddtbt05, ddtbt06, ddtbt09) exhibit large erosion cavities in the endosteal part of the cortex (Figs 4 and 5). The resorption cavities have an area of between 0.01 mm² and 0.98 mm², which corresponds to 0.1% to 5.9% of the total bone area (Supplementary Table S3). In some of these enlarged cavities, a centripetal deposition of lamellar bone occurs (Fig. 4b,c).
The youngest individuals in our sample appear to be late stage juveniles. A large proportion of their compacta comprises fibrolamellar bone, which suggests that during early ontogeny bone deposition occurred at a rapid rate13, 21. Such rapid rates of growth during the early growth stages is typical for modern birds; it has also been described in other species such as the secretary bird (Sagittarius serpentarius)14, Japanese quail (Coturnix japonica) 22, king penguin (Aptenodytes patagonicus)23 and ostrich (Struthio camelus)14, as well as in Mesozoic ornithurine birds13, 24, 25. The well-developed ICL in the youngest dodo specimens suggests that medullary expansion had already been completed in these individuals13.
During later stages of ontogeny, the fibrolamellar bone, which formed during early ontogeny, is reconstructed and remodeled13, 14. The compacta of adult individuals show a well-developed ICL and OCL, both consisting of more slowly formed lamellar bone tissue. The formation of the OCL tends to occur after the development of the ICL, and only once sexual maturity has been attained14, 22. This three-layer arrangement of the tissue in the bone wall in the dodo is similar to that described by Enlow and Brown26 for a modern hawk (Buteo), chicken (Gallus), turkey (Meleagris), guinea fowl (Numida) and crow (Corvus), and has been shown for other birds such as secretary bird S. serpentarius 14 and albatross Diomedea 13.
Like large modern flightless birds, e.g. cassowary (Casuarius)27, ostrich (Struthio)14 and rhea (Rhea)28, the dodo also has rapidly formed fibrolamellar bone tissue. However, unlike these birds in which this is the predominant tissue of the bone wall, in the dodo this tissue makes up only one third of it. Slower, more protracted rates of growth during ontogeny have been documented for moas (Dinornithiformes)27, the kiwi (Apteryx)29, and the Late Cretaceous Gargantuavis 30. The slower growth to adult size appears to be related to the lack of predators and to environmental resource stress22, 27, 29, 30. Interestingly, although the dodo does not exhibit the slow protracted growth of these large island birds, it does appear to have had an extended growth period when reaching skeletal maturity, which is confirmed by the presence of several LAGs in the OCL. Thus, the dodo experienced rapid growth rates until the attainment of sexual maturity, but thereafter it took several years to attain skeletal maturity. Such an extended, slow growth after sexual maturity might have been possible on a small island like Mauritius24 where until the arrival of humans, adult birds lacked any natural predators.
Among modern birds, when lines of arrested of growth are present, they tend to be restricted to the OCL13. However, terrestrial birds on islands, like the kiwi29 or Dinornithiformes27, have several LAGs throughout the cortex. Turvey et al.27 proposed that the LAGs in the slow growing Dinornithiformes, and in particular Megalapteryx didinus are the result of severe environmental fluctuations27. Likewise, Köhler et al.31 suggested that the frequent observation of LAGs in wild ruminants were the result of seasonality. In addition, the growth lines evident in the insular Myotragus is considered to be the result of resource limitation32. Experimental studies on birds that were given restricted access to food have demonstrated that although such limitations did not necessarily produce LAGs, they resulted in a decrease in bone depositional rates22. However, if access to food was more severely limited, LAGs may have resulted; this has been suggested for another insular flightless columbiform, the solitaire Pezophaps solitaria 32. In the case of the dodo, harsh seasonal conditions on Mauritius exist during the summer months, between November and March. During this period, cyclones can occur, during which heavy rain and strong winds can strip trees of leaves, flowers and fruit, causing severe food shortages and starvation for the island fauna, and it can take a few months for normal environmental conditions to return3, 33, 34. These events may explain the variations in body mass, as reported by mariners for the dodo8. As these seasonal events and consequent long food shortages could result in nutritional stress, we suggest that the LAGs observed in the OCL in the dodo bones were produced in the summer months, between November and March.
The presence of medullary bone in two specimens proves unequivocally that part of the sample were ovulating females35. The tibiotarsus (ddtbt08) shows a large area of medullary bone in comparison to the femur (ddfem04). This could be a result of histological variability between these skeletal elements13, although they could also suggest differences in their reproductive cycles, i.e. one bird (represented by ddtbt08) had not yet utilized the medullary bone for eggshell production, while the other bird (represented by ddfem04) had already shelled its eggs13, 21, 36. Livezey37 had previously used the K-means statistical method to deduce sexual dimorphism in the dodo; however he did not statistically check the validity of the clusters obtained using the K-means methodology, which therefore invalidates them. Apart from this work, the dodo is not considered to be overly sexually dimorphic, and in the current study we found that the circumferences of the bones of the females were similar to other adult bones in the sample (Supplementary Tables S2–S3). Thus, as for the extinct birds Confuciusornis 35 and Dromornis 38, bone histology has proved to be an important tool in sex determination of the dodo.
The adult dodo specimens generally show a large amount of secondary reconstruction, with many secondary osteons present in the cortex, as well as evidence of extensive resorption around the medullary cavity13. However, five specimens (ddfem03, ddtbt02, ddtbt05, ddtbt06, ddtbt09), have much enlarged cavities in the cortex suggesting that the demand for calcium surpassed the normal requirements. Such significant bone remobilization has been reported in penguins during molting when the demand for calcium is increased39, 40, as well as, for 15 species of modern birds including the domestic pigeon40. Periodic molting is common among modern birds34, 41, 42, which allows time for the replacement of old damaged feathers, and commonly occurs after breeding3, 33, 34, 41, 42. Molting periods have been previously proposed for the dodo43,44,45,46,47, but these are generally unsupported48. On the basis of earlier osteohistological observations made on several other birds species39, 40, we suggest that the presence of extensive resorption in the bones of the dodo could be interpreted as evidence of molting.
We further propose that since molt can generate significant changes in the appearance of birds in terms of color and feather type3, this may explain the many discrepancies in the descriptions of the dodo in historical accounts8, 49. The dodo was variably described as having “three or four black quills” in the place of their wings, and a tail with “four or five small curled plumes of a greyish color”49. Some other descriptions of the dodo mention a “clothing of downy feathers” or even “no feathers on their body, which is covered in black down”49. Thus, we propose that mariners may have been describing the dodo at different stages of molt. Dodos described as having a downy plumage49 were probably observed and described just after molt, as seen in modern birds in a similar condition3, 34, 41, 42, whilst the grey or black plumage49 could correspond to dodo specimens between two molting periods50.
Our study of bone microstructure has given insight into the molting and reproductive behavior of the dodo, but the timing of these events needs further clarification. Since Mauritius has a seasonal cycle of cyclonic events, which occurs between November and March (austral summer), Staub44 suggested that the dodo bred in the austral winter, between March and September, when food was abundant, with a post-breeding molting period between September and December. He based this hypothesis on a description by Captain van Westzanen in 1602, who described the meat of the dodo as a very tasty food. Staub44 considered that this culinary aspect was a result of the birds eating a profusion of fruits from the endemic palm trees (Latania sp., Dictyosperma sp., Hyophorbe sp.), which produced fruits during the winter. Thus, he suggested, that the dodo evolved its reproduction period to coincide with food abundance, following the same reproductive strategy of the solitaire on neighboring Rodrigues Island. However, if we compare this annual molting pattern proposed by Staub44 with information known about modern surviving birds on Mauritius, his hypothesis is improbable. On Mauritius, (contra Staub), all birds breed during the austral summer (between August and January, which sometimes extends into March), and start the post-breeding molt between November and March3, 33, 34, 41. Moreover, the Pink Pigeon (Nesoenas mayeri), which has the longest molting period of any bird on Mauritius, does not molt during the months towards the end of the austral winter (June to October) when food resources are limited33.
Thus, bone histology provides significant information about the timing of breeding and molting periods in the dodo. We can now consider that as LAGs form during the cyclone period between November and March, the breeding and the molting periods can be accurately determined using the amount of bone deposited after the LAG (Fig. 6.1). Assuming that the bone deposition rate is constant between consecutive LAGs, we propose that the thicker the bone deposition between two LAGs, the more time has lapsed since the LAG formed, thereby giving an indication of when the breeding or molting event occurred. Furthermore, if we examine the external cortex of the two specimens that have formed medullary bone (ddtbt08 and ddfem04), it is evident that a relatively large amount of bone had formed after the last LAG. This new bone in the OCL, corresponds to around 50% of the bone deposited between the two previous LAGs (Fig. 2b). Considering that LAGs are formed annually, we can estimate that ovulation happens approximately six months after the last LAG, i.e. about six months after the last austral summer, at the beginning of August (Fig. 6.5). We hypothesize that after egg laying and hatching (Fig. 6.6), the chicks grew rapidly to reach a relatively large size so that they were better able to withstand the environmental stress of the next austral summer (Fig. 6.7), which usually corresponds to the cyclone period, and hence resource limitations.
The external cortex of specimens that exhibit evidence of molting in their bone (i.e., several large resorption cavities) have thin lamellar deposits after the last LAG in the OCL (Figs 4 and 5), which further suggests that molting starts just after the last austral summer (Fig. 6.2). Therefore, molting occurred after the breeding and cyclone season, allowing for the renewal of plumage before the next breeding period. This is congruous with the variable descriptions of the plumage of the dodo by mariners in which they described dodos with a downy body and some feathers in the wings and the tail49 during June and July. These descriptions likely correspond to the middle of the molting period, when all old feathers have already been lost and replaced by temporary downy plumage (Fig. 6.3). The new feathers then appear, starting with the wings and the tail, as seen in modern birds3. Mariners further described a plumage composed of real feathers in the documents written in July and September49. This means that at the end of July the molt is completed and the breeding season begins with a new plumage (Fig. 6.4). On the basis of this evidence we propose that the dodo molt occurred between March and the end of July, after the austral summer and cyclone period, and before the next breeding season, which is consistent with the breeding and molting periods described for all surviving birds on Mauritius3, 33, 34, 41.
This study of the bone histology of the dodo provides insight into the life history of this recently extinct bird. In order to deduce the timing of the events such as reproduction and molting we have considered the histological patterns, modern birds in Mauritius and the ecology of the area. From these we propose that the breeding season started several months before the austral summer (around August) with ovulation in the females, and that it occurred after a period of potential fattening, which corresponds with the fat and thin cycles recorded in many Mauritian vertebrates, both living and extinct. We further suggest, that after the eggs were laid and chicks hatched, they grew quickly to almost adult body size and attained sexual maturity before the cyclone period in the austral summer. Additionally, our findings could indicate that following the breeding season and the end of the austral summer, molting began (around March) with the replacement of the feathers of the wings and the tail first. Thus, at the end of July, the molt would have been completed in time for the next breeding season. These novel findings about the life history of the dodo have been deduced from the bone microstructure and the proposed timing thereof appear to correlate well with the current observations of modern birds in Mauritius, and have been further corroborated by historical descriptions.
Materials and Methods
Twenty-two sections from the diaphysis of dodo bones were sampled (Supplementary Table S1). The material corresponds to 22 bones, including five femora, fourteen tibiotarsi, two tarsometatarsi, and one humerus. These bones are from different fossil localities on Mauritius. The brown specimens are from the swamp named Mare aux Songes (MAS) in the south-east of the island. Some of these are held at Omnicane, Mauritius (formerly Mon Trésor Mon Désert sugar estate) and were loaned to NHM (Natural History Museum, London) for thin sectioning. Some were loaned from the Muséum d’Elbeuf (Normandie, France). The latter were discovered by the Mauritian naturalist, Paul Carié, owner of the Mare aux Songes during the 19th century, and remained unknown until 2014, when the descendants of Carié donated them to the Muséum d’Elbeuf. The four white tibiotarsi are from different caves in the island. Although these specimens are fragmentary and unassociated, comparison with the Strickland and Melville5 anatomical descriptions of the dodo permitted their unambiguous identification.
All the bones were sectioned in transverse section with a handheld Dremel sectioning tool in the middle of the diaphysis. For specimen “ddhu1”, in which the middle part of the diaphysis was missing, the section was taken in the most proximal part. The sectioned bits of bone were pretreated by soaking in six, eight hour baths of ethanol 95%, followed by three baths of acetone 95%, to remove the organic remains. The bone samples were thereafter embedded in Struers epoxy resin and then thin sections were prepared using an Imptech PC10 thin sectioning machine, and the final polishing was completed using silicon dioxide power on a velvet cloth13, 51. The thin sections were examined under a Nikon Eclipse Biological petrographic microscope E200. Microphotographs and measurements were performed using NIS Elements version 3.0 and Bone Profiler version 4.5.8.
Shapiro, B. et al. Flight of the dodo. Science 295, 1683–1683 (2002).
Moree, P. J. A Concise History of Dutch Mauritius, 1598–1710: A Fruitful and Healthy Land (1998).
Cheke, A. S. In Studies of Mascarene Island Birds (ed. Diamond, A. W.) 5–89 (1987).
Hume, J. P., Martill, D. M. & Dewdney, C. Dutch diaries and the demise of the dodo. Nature 492, 622 (2004).
Strickland, H. E. & Melville, A. G. The dodo and its kindred; or the history, affinities and osteology of the dodo, solitaire and other extinct birds of the islands Mauritius, Rodriguez, and Bourbon. Lond. Reeve Benham Reeve (1848).
Claessens, L. P., Meijer, H. J. & Hume, J. P. The morphology of the Thirioux dodos. J. Vertebr. Paleontol. 35, 29–187 (2015).
Janoo, A. Discovery of isolated dodo bones [Raphus cucullatus (L.), Aves, Columbiformes] from Mauritius cave shelters highlights human predation, with a comment on the status of the family Raphidae Wetmore, 1930. Annls. Paléont. 91, 167–180 (2005).
Hume, J. P. The history of the dodo Raphus cucullatus and the penguin of Mauritius. Hist. Biol. 18, 65–89 (2006).
Angst, D., Buffetaut, E. & Abourachid, A. The end of the fat dodo? A new mass estimate for Raphus cucullatus. Naturwissenschaften 98, 233–236 (2011a).
Angst, D., Buffetaut, E. & Abourachid, A. In defence of the slim dodo: a reply to Louchart and Mourer-Chauviré. Naturwissenschaften 98, 359–360 (2011b).
Louchart, A. & Mourer-Chauviré, C. The dodo was not so slim: leg dimensions and scaling to body mass. Naturwissenschaften 98, 357–358 (2011).
Brassey, C. A., O’Mahoney, T. G., Kitchener, A. C., Manning, P. L. & Sellers, W. I. Convex-hull mass estimates of the dodo (Raphus cucullatus): application of a CT-based mass estimation technique. PeerJ 4, e1432, doi:10.7717/peerj.1432 (2016).
Chinsamy-Turan, A. The microstructure of dinosaur bone: deciphering biology with fine-scale techniques (John Wiley & Sons, 2005).
Chinsamy, A. Histological perspectives on growth in the birds Struthio camelus and Sagittarius serpentarius. Cour. Forschungsinstitut Senckenberg 181, 317–323 (1995).
Werning, S. The ontogenetic osteohistology of Tenontosaurus tilletti. PLoS One 7, e33539, doi:10.1371/journal.pone.0033539 (2012).
Horner, J. R., De Ricqlès, A. & Padian, K. Long bone histology of the hadrosaurid dinosaur Maiasaura peeblesorum: growth dynamics and physiology based on an ontogenetic series of skeletal elements. J. Vertebr. Paleontol. 20, 115–129 (2000).
Ponton, F. et al. Variation of the outer circumferential layer in the limb bones of birds. Acta Ornithol. 39, 137–140 (2004).
Cormack, D. H. Ham’s histology (Lippincott, 1987).
Woodward, H. N., Horner, J. R. & Farlow, J. O. Osteohistological evidence for determinate growth in the American alligator. J. Herpetol. 45, 339–342 (2011).
Amprino, R. Bone Histophysiology. Guys Hosp. Rep. 116, 51–69 (1947).
Dacke, C. G. et al. Medullary bone and avian calcium regulation. J. Exp. Biol. 184, 63–88 (1993).
Starck, J. M. & Chinsamy, A. Bone microstructure and developmental plasticity in birds and other dinosaurs. J. Morphol. 254, 232–246 (2002).
de Margerie, E. et al. Assessing a relationship between bone microstructure and growth rate: a fluorescent labelling study in the king penguin chick (Aptenodytes patagonicus). J. Exp. Biol. 207, 869–879 (2004).
Chinsamy, A. & Elzanowski, A. Bone histology: evolution of growth pattern in birds. Nature 412, 402–403 (2001).
Wilson, L. E. & Chin, K. Comparative osteohistology of Hesperornis with reference to pygoscelid penguins: the effects of climate and behaviour on avian bone microstructure. R. Soc. Open Sci. 1, 1–16 (2014).
Enlow, D. H. & Brown, S. O. A comparative histological study of fossil and recent bone tissues. Part II. Tex. J. Sci. 9, 186–204 (1957).
Turvey, S. T., Green, O. R. & Holdaway, R. N. Cortical growth marks reveal extended juvenile development in New Zealand moa. Nature 435, 940–943 (2005).
Chinsamy, A., Chiappe, L. M. & Dodson, P. Mesozoic avian bone microstructure: physiological implications. Paleobiology 21, 561–574 (1995).
Bourdon, E. et al. Bone growth marks reveal protracted growth in New Zealand kiwi (Aves, Apterygidae). Biol. Lett. 5, 639–642 (2009).
Chinsamy, A., Buffetaut, E., Canoville, A. & Angst, D. Insight into the growth dynamics and systematic affinities of the Late Cretaceous Gargantuavis from bone microstructure. Naturwissenschaften 101, 447–452 (2014).
Köhler, M. & Moyà-Solà, S. Physiological and life history strategies of a fossil large mammal in a resource-limited environment. Proc. Natl. Acad. Sci. 106, 20354–20358 (2009).
Steel, L. Bone histology and skeletal pathology of two recently extinct flightless pigeons: Raphus cucullatus and Pezophaps solitaria. J. Vertebrate Paleontol Abstract. 29, 185 (2009).
Jones, C. G. In Studies of Mascarene island birds (eds Diamond, A. W.) 208–300 (1987).
Safford, R. J. The annual cycle and breeding behaviour of the Mauritius fody Foudia rubra. Ostrich 68, 58–67 (1997).
Chinsamy, A., Chiappe, L. M., Marugán-Lobón, J., Chunling, G. & Fengjiao, Z. Gender identification of the Mesozoic bird Confuciusornis sanctus. Nat. Commun. 4, 1–5 (2013).
Wilson, S. & Thorp, B. H. Estrogen and cancellous bone loss in the fowl. Calcif. Tissue Int. 62, 506–511 (1998).
Livezey, B. C. An ecomorphological review of the dodo (Raphus cucullatus) and solitaire (Pezophaps solitaria), flightless Columbiformes of the Mascarene Islands. J. Zool. 230, 247–292 (1993).
Handley, W. D., Chinsamy, A., Yates, A. M. & Worthy, T. H. Sexual dimorphism in the late Miocene mihirung Dromornis stirtoni (Aves: Dromornithidae) from the Alcoota Local Fauna of central Australia. J. Vertebr. Paleontol. e1180298, doi:10.1080/02724634.2016.1180298 (2016).
Dabee, V. P. Comparison of the long bone microstructure of two southern African marine birds, the Cape gannet (Morus capensis) and the African penguin (Spheniscus demersus) with respect to their aquatic adaptations (University of Cape Town, 2013).
Meister, W. Changes in histological structure of the long bones of birds during the molt. Anat. Rec. 3, 1–21 (1951).
Safford, R. J. & Beaumont, J. Observations on the biology of the Mauritius cuckoo-shrike Coracina typica. Ostrich 67, 15–22 (1996).
Safford, R. J. Notes on the biology of the Mauritius black bulbul Hypsipetes olivaceus. Ostrich 67, 151–154 (1996).
Hachisuka, M. Dodo and kindred birds; or The extinct birds of the Mascarene Islands (1953).
Staub, F. Fauna of Mauritius and associated flora (1993).
Staub, F. Dodo and solitaires, myths and reality. Proc. R. Soc. Arts Sci. Maurit. 6, 89–122 (1996).
Lüttschwager, J. Zur systematischen Stellung der ausgestorbenen Drontevögel Gattung Raphus (Didus) und. Pezophaps. Zool. Anz. 162, 127–148 (1959).
Grihault, A. Dodo: the bird behind the legend. (Imprimerie & Papeterie Commerciale, 2005).
Parish, J. C. The dodo and the solitaire: a natural history. (Indiana University Press, 2013).
Fuller, E. Dodo: from extinction to icon. (Collins, 2002).
Brom, T. G. & Prins, T. G. Microscopic investigation of feather remains from the head of the Oxford dodo. Raphus cucullatus. J. Zool. 218, 233–246 (1989a).
Chinsamy, A. & Raath, M. A. Preparation of fossil bone for histological examination. Paleontol. Afr. 29, 39–44 (1992).
We acknowledge Jérôme Tabouelle, curator of the Muséum d’Elbeuf for the access to the material, and we also acknowledge the Paul Carié family for their donation of the large collection of dodo bones to the Muséum d’Elbeuf. We also acknowledge Omnicane (formerly Mon Trésor Mon Désert sugar estate, MTMD) in Mauritius for the loan of a part of the bones, and the Dodo Research Program participants of the excavations of these bones. We are grateful to the reviewers for their constructive comments which have improved the manuscript.
The authors declare that they have no competing interests.
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Angst, D., Chinsamy, A., Steel, L. et al. Bone histology sheds new light on the ecology of the dodo (Raphus cucullatus, Aves, Columbiformes). Sci Rep 7, 7993 (2017). https://doi.org/10.1038/s41598-017-08536-3
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