Correlation between the levels of SjHSP60 antibodies and the sizes of the hepatic granulomas in the S. japonicum-infected rabbits 8 weeks after infection. (A) Rabbits were infected with or without 60 S. japonicum cercariae. Eight weeks later, the sera were collected, and anti-SjHSP60 total IgG antibody was determined by ELISA in triplicates. The results are presented as the means ± the SDs from six rabbits in each group and are representative of 3 independent experiments. ***P < 0.001 (Student’s t-test). (B) Sera and liver slices were obtained from rabbits infected with 30 or 60 S. japonicum cercariae. The associations of the titers of SjHSP60 IgG antibody with the sizes of the granulomas in S. japonicum-infected rabbits were determined by Spearman’s rank correlation. The sizes of the granulomas around single eggs were quantified with the AxioVision Rel 4.7. The data are expressed in area units (n = 14). Anti-SjHSP60 total IgG antibody was determined by ELISA. The data are expressed in serum dilution (1/x). The data are representative of 3 independent experiments. Abbreviation: SjHSP60, S. japonicum heat shock protein 60.