Nanoparticle-based Plasmonic Transduction for Modulation of Electrically Excitable Cells

There is a compelling need for the development of new sensory and neural prosthetic devices which are capable of more precise point stimulation. Current prosthetic devices suffer from the limitation of low spatial resolution due to the non-specific stimulation characteristics of electrical stimulation, i.e., the spread of electric fields generated. We present a visible light stimulation method for modulating the firing patterns of electrically-excitable cells using surface plasmon resonance phenomena. In in-vitro studies using gold (Au) nanoparticle-coated nanoelectrodes, we show that this method (substrate coated with nanoparticles) has the potential for incorporating this new technology into neural stimulation prosthetics, such as cochlear implants for the deaf, with very high spatial resolution. Au nanoparticles (NPs) were coated on micropipettes using aminosilane linkers; and these micropipettes were used for stimulating and inhibiting the action potential firing patterns of SH-SY5Y human neuroblastoma cells and neonatal cardiomyocytes. Our findings pave the way for development of biomedical implants and neural testing devices using nanoelectrodes capable of temporally and spatially precise excitation and inhibition of electrically-excitable cellular activity.

between light and conduction band electrons of metal nanoparticles, leading to potential alternatives to electrical excitation, used in current biomedical implants. To utilize the LSPR fields for cell stimulation, sufficient amount of nanomaterial has to be extremely close to the targeted tissue; various methods have been employed to achieve this like surface modification of nanoparticles, bio-conjugation and local delivery via injection. For instance, Carvalho-de-Souza et al. 14 conjugated Au nanoparticles with three different ligands -Ts1 neurotoxin and two antibodies (targeting TRPV1 and P2 X 3 channel receptors) respectively, and successfully bound the particles to dorsal ganglion neurons (DRGs), then stimulated the DRGs with 532 nm green laser light. Li et al. 16 utilized photosensitive hydrogel embedded with polypyrrole (PPy) nanoparticles to release biomolecule transmitters (glutamate for excitation & DNQX for inhibition). A 980 nm infrared (IR) laser light was used to excite hippocampal neurons in-vitro when glutamate was released and to inhibit responses from the rat visual cortex in-vivo when DNQX was released. Yoo et al. 19 coated Au nanorods with polyethylene glycol (PEG) that facilitates binding to the cell membrane and used a 785 nm near infrared laser to invoke inhibition in rat hippocampal tissues. Eom et al. 15 conjugated Au nanorods to rat sciatic nerve and recorded compound nerve action potential, in response to a 980 nm IR laser. Au nanorods were injected into the sciatic nerve using a glass capillary and nerve bundle was excised subsequently. Similarly, Yong et al. 18 incubated primary auditory neurons with silica-coated Au nanorods overnight and excited the neurons with a 780 nm near IR laser. The above mentioned studies have one common feature in their methodologies, i.e., the modification of nano-neural interfaces to achieve the stimulation. The major drawback with alteration of nano-neural surfaces is that in vivo translation raises issues regarding unwanted toxicity, repeatability and bio-compatibility. For example, excessive heating by infrared lasers can damage healthy tissues. Hence, there is need to find more viable ways, which minimize collateral damage, to use in vivo for translation into new neural prosthetic and testing devices.
Here, we report an Au nanoeletrode (Au nanoparticle-coated glass micropipette) which does not need any surface modification or bio-conjugation for neural stimulation via visible-light lasers. The nanoelectrodes were characterized via electron microscopy and validated for generation of plasmonic responses via light-induced photocurrents and fluorescence quenching experiments as proof of concept before the cellular physiology experiments. Subsequently, we stimulated two different cells, SH-SY5Y human neuroblastoma a cell line that has characteristics of neurons, and neonatal cardiomyocytes, with a nanoelectrode and a 532 nm green laser. These experiments served as initial, in vitro proof of concept that wireless nanoelectrodes in combination with visible light can be used instead of electrical electrodes or infra-red (IR) lasers, for precise temporal modulation of neural and cardiac cellular responses. Based on these initial breakthrough results, we visualize that future biomedical implants based on LSPR phenomena using nanoelectrodes and light will give superior spatial resolution and more clinically useful focal stimulation. Implantable electrodes such as cochlear implant electrode arrays, which use polymeric materials are easily designed using the fundamental results demonstrated in this contribution.

Results
Nanoelectrode Characterization and Testing. Scanning electron microscopy (SEM) images of the nanoelectrodes (Fig. 1a) show the uniform coating of Au NPs on the outer surface of the electrode tip. Au NPs synthesized via a liquid phase route were characterized using transmission electron microscopy (TEM) (Fig. 1b) and UV-Vis spectrophotometry. The LSPR absorption peak at 528 nm confirms the presence of Au NPs (Fig. 1c) of approximately 20 nm diameter (Fig. 1b). Prior to experiments with biological cells, the nanoelectrodes were tested using an electrochemical cell having 0.1 M phosphate buffer with 0.05 M EDTA (Fig. 2a). Photocurrents were observed during the illumination of the Au nanoelectrode with 532 nm green light, while there were no photocurrents for uncoated glass micropipettes (Fig. 2b). Lowe et al. 20 demonstrated similar photocurrents for Au NP-coated indium tin oxide electrodes and attributed it to the temperature rise at the nanoelectrode-electrolyte interface by plasmonic excitation of Au nanoparticles. The temperature rise at the interface led to alteration of the open circuit voltage of the nanoelectrode with respect to the reference electrode, resulting in photocurrents. These photocurrents vary linearly with applied voltage (Fig. 2d). Photocurrents were also recorded when an extracellular solution (ECS) having a composition of 140 mM NaCl, 5 mM KCl, 1 mM MgCl 2 , 2 mM CaCl 2 , 10 mM HEPES at pH 7.4 maintained with NaOH, was used as the electrolyte instead of phosphate buffer (Fig. 2c) which also exhibited a linear relation with applied voltage (Fig. 2e). Due to SPR, gold nanoparticles are known to affect the emission spectra of fluorophores in its vicinity via energy transfer between the two and are utilized in many fluorescence-based applications like molecular imaging, and sensing [21][22][23][24][25][26] . Aslan et al. 21 studied the effect of Au NP size on emission spectra of FITC-labeled human serum albumin. We adapted the same methods for testing the nanoelectrodes for fluorescence quenching by the Au NPs 21,22 . FITC-labeled human serum albumin (HSA) was attached to the Au NP-coated micropipette by incubating the nanoelectrode with a 10 μM solution of the fluorophore. A 488 nm laser source was used to excite FITC-HSA and the emission spectrum the wavelength of ~520 nm was observed under an Olympus BX61W1 upright microscope. Fluorescence quenching was observed in the presence of Au NPs ( Supplementary Fig. S1b,c) in comparison to uncoated glass micropipette tips ( Supplementary Fig. S1a).
The ECS solution was heated to approximately 55 °C, then, allowed to cool down to room temperature. The temperature was monitored using a thermistor. The resistance of a patch pipette filled with the same ECS was recorded as the solution cooled down. Supplementary Fig. S2c represents the log (R) vs 1/temperature calibration curve, fitted to a straight line [log R = 868.5 × (1/T) + 3760; R 2 = 0.9027]. For plasmonic temperature measurement, a patch pipette was placed just next to the nanoelectrode and 20 mV voltage pulses were applied to the patch pipette. The change in resistance was measured in response to stimulation with a 100 mW, 532 nm green laser shined on the tip of the nanoelectrode (Supplementary Fig. S2a). There was no change in resistance for the control condition (no nanoelectrode) ( Supplementary Fig. S2b). This resistance change was converted into temperature changes using the calibration curve ( Supplementary Fig. S2c). Notice that the plasmonic temperature increases linearly with the laser power (Fig. 3b,d) and decreases exponentially with the distance away from the nanoelectrode (Fig. 3c). A 0.3 to 0.4 ( ± 0.1) °C rise was observed when measured with a thermal camera ( Supplementary Fig. S3). These experiments confirm the plasmonic temperature rise around the nanoelectrode when illuminated with green visible laser.

Excitatory Neural Responses.
The experiments were done with SH-SY5Y cells in the standard whole cell current clamp configuration using a patch clamp system. The holding current was adjusted to set the membrane potential to a particular baseline value. Light was focused on the electrode via an optical fiber and membrane conductance were recorded using a patch electrode ( Supplementary Fig. S4a). The nanoelectrode was placed within 2 µm of the cell membrane. When the nanoelectrode was illuminated with a 532 nm, 100 mW laser for 10 ms or more, a shift in the cell membrane potential was observed, and changed as a function of the cell holding potential (Fig. 4a). Specifically, for −30 mV or less, there were positive shifts in the membrane potential and as the membrane potential approached zero, the magnitude of potential shifts decreased and became negative for positive holding potentials. Fig. 4a shows an example for a 10 ms, 100 mW laser pulse. It can be seen that at a cell membrane potential of −73.7 mV (applied holding current = −55 pA), there was positive shift in the evoked membrane potential -blue curve, and, for a positive cell potential of +23.6 mV (holding current = +53 pA), there was negative shift -pink curve. The SH-SY5Y cells fired typical action potentials in response to electrical stimulation before and after the optical simulation was applied to each cell. Fig. 4b shows the paired relations between shifts in evoked potential in response to laser-nanostimulation and the baseline potential (determined by applied current) for six different cells, each cell shown by a different colour. The positive shifts in evoked membrane potential indicates partial depolarization and negative shifts indicates partial hyperpolarization. We observed that the magnitude of plasmonic jumps increased with an increase in laser power ( Supplementary  Fig. S4b) and also with increase in laser pulse duration (10-50 ms) ( Supplementary Fig. S4c). Similar shifts in evoked potential jumps were observed for neonatal rat cardiomyocytes ( Supplementary Fig. S4d). For laser pulse widths of 1-5 ms, action potentials were recorded from the cells. Fig. 4c shows action potential recordings for a representative SH-SY5Y cell when the nanoelectrode was illuminated with a 1 ms 532 nm green laser pulse with 100 mW power. Fig. 4d shows these optically stimulated action potential recordings from six different cells. To induce optical action potentials, we found that laser power should be in the 75 mW-120 mW range. At lower powers below 60 mW, no cellular responses were observed. There was no difference between electrically-evoked action potentials prior to and after the optical stimulation in response to electrical current pulses ( Supplementary  Fig. S4e). For control experiments (laser stimulation alone -without nanoeletrode −10 ms, 100 mW), no evoked potentials were observed ( Supplementary Fig. S6). Following stimulation the membrane potential slowly returns to pre-stimulus values over a time span of seconds and up to a minute. Carvalho-de-Souza et al. 14 also reported

Inhibitory Cell Responses.
Like activation experiments, inhibition experiments were also performed in whole cell current clamp configuration. The laser pulses were delivered using the optical fiber and the current pulses were delivered via patch pipette ( Supplementary Fig. S4a). Action potentials were evoked in SH-SY5Y cells using 180 pA, 300 ms current pulses. During simultaneous presentation of optical pulses (300 ms, 120 mW), we observed decreases in the magnitude and rate of the action potentials as compared to the control conditions, i.e., electrical stimulation alone. Action potentials were recorded before and after the optical stimulation experiments (Fig. 5a) and we found no deleterious effects. Similar inhibition of action potential magnitude was observed for spontaneous beating neonatal rat cardiomyocytes ( Supplementary Fig. S5). As laser power increased, the inhibition becomes more prominent (Fig. 5b). The inhibition was most prominent when the laser pulse led the electric current pulse by a few milliseconds (5-15 ms) (Fig. 5c). Typically for action potentials, depolarization happens predominantly because of Na + influx and repolarization happens mainly because of K + efflux. Different data analyses were carried out with respect to laser power for depolarization and repolarization phases of the action potentials as shown in Fig. 6a: action potential peaks (AP peaks), up-slope of the AP (rate of the membrane potential rise from baseline potential to peak value: rate of depolarization), normalized up-slope (all the up-slope values are normalized by dividing each value with the initial value of the up-slope for electrical action potentials, recorded before the optical stimulation experiment), differences between the initial peak value and first minimum of the action potential (AP Peak -Base), base value (first minimum after peak value), the down-slope of the AP (rate of membrane potential fall from the peak value to base value: rate of depolarization) and normalized downslope of AP (like up-slopes, downslopes were also normalized with initial value of downslope for electrical action potentials, recorded before the optical stimulation experiment). The AP peak value decreased as laser power increased (Fig. 6b). The up-slope (absolute and normalized) depolarization rate, remained unaffected by the laser power ( Fig. 6c,d) while the down-slope (absolute and normalized) repolarization rate, decreases with increases in laser power (Fig. 6g,h). The mean data also confirms the same trends with laser power (Supplementary Fig. S7). As depolarization is mostly governed by Na + influx and repolarization is governed by K + efflux, it is possible that during inhibition, the degree of K + efflux was affected more than the rate of Na + influx. The minimum after the AP peak, i.e., base value did not change with increases in laser power (Fig. 6f), and the difference between the peak & base values decreased with laser power (Fig. 6e).

Discussion
We have demonstrated for the first time that Au nanoelectrodes in combination with a 532 nm green laser can stimulate and inhibit neural and cardiac action potentials. This is the first report showing both inhibition as well as activation of action potentials using Au NPs coated micropipettes and a visible-light laser. We demonstrated these phenomena for two cell models -the human neuroblastoma cell line and neonatal cardiomyocytes. In addition, we were able to record both inhibitory and excitatory responses from the same cells, depending on the properties of laser stimulation (e.g., Fig. 7). We found that to elicit action potentials, the laser timing is a critical parameter. In the present investigation, the cells fired action potentials for short laser pulses with 1-5 ms durations ( Fig. 4c and d). For longer pulses (10 ms or higher), though there was a shift in the evoked cell potential in response to the laser stimulation, no excitatory action potentials were elicited (Figs 4a and b, Supplementary S4b, c & d). In the case of opto-electrical stimulation (laser pulse with electric current pulse for stimulation -timing 300 ms) of SH-SY5Y cells, we discovered that inhibition of action potentials happens ( Fig. 5a and b), an observation consistent with our experiments with spontaneous beating cardiomyocytes, i.e., decreases in the magnitude of action potentials were elicited (laser pulse timing = 10 s) ( Supplementary Fig. S5). Certain aspects of the discoveries here are consistent with recent reports in the literature. A few previous studies report neural excitatory activation with pulse widths of a few milliseconds 14,15 , and for inhibition, the laser durations were for longer time periods (tens to hundreds of milliseconds) 19,27 . Specifically, Yoo et al. 19 reported inhibition of neural activity for hippocampal tissue slices when stimulated with gold nanorods and a 785 nm near-IR laser. The laser pulse durations were from several seconds to a few minutes. We observed similar inhibition of neural activity in our plasmonic stimulation experiments but only for longer (300 ms) laser pulse durations. Similarly, Eom et al. 15 showed neural activity enhancement utilizing IR stimulation for rat sciatic nerve when the nerve was stimulated in the presence of Au nanorods, as compared to the control condition (laser with no Au nanorods). The laser pulse duration was less than 3 milliseconds, a similar duration for which we observed neural activation (1-5 ms) in the present study. What is exciting about the discoveries we present here is the possibility of stimulating using electrodes and visible light, leading to translation to prosthetics such as cochlear implant electrodes constructed of suitable polymer materials.
To make significant progress towards clinical applications, it is important to move towards understanding the biological mechanisms for light-activated Au NPs neural stimulation. Metal nanoparticles like Au have strong surface interactions with electromagnetic fields -light, due to availability of free electrons in their conduction bands. For small size particles (<20 nm) nearly all energy is absorbed and converted into heat 28,29 . Since we used particles of approximately 20 nm, it is likely that non-radiative decay (localized plasmonic heating) plays a critical role in the neural stimulation reported here. In addition, there are recent reports that IR lasers can invoke in vivo responses [30][31][32][33][34] . A likely mechanism for IR stimulation is a temperature rise due to photothermal interactions with cell membrane capacitance properties 35,36 . In particular, Shapiro et al. 35 showed that the absorption of IR laser energy, causing a temperature rise, resultant changes in cell membrane capacitance are sufficient for cell depolarization. The artificial bilayer was used as the model of the cell membrane, and capacitance changes were evaluated in response to IR stimulation. So, it is very likely that localized plasmonic heating due to Au nanoelectrode interactions with visible light alters the cell capacitance. Carvalho-de-Souza et al. 14 also suggested a similar mechanism to explain how conjugated Au NPs enables action potential activation in dorsal root ganglion neurons. Like Shapiro et al. 35 , an artificial bilayer was considered as a representative model of cell membrane capacitance changes. In sum, initial reports suggest that membrane capacitance change is the driving force for action potential generation in response to short thermal pulses. Apart from the transient capacitance change mechanism, there are indirect indications that heat sensitive channels could also play a role in depolarization 37 .
One more possible physical effect of the plasmonic stimulation is alteration of cell membrane properties by the thermal pulse. This could potentially involve transient nanopore formation in the cell membrane, facilitating a nonspecific ion permeability increase, altering the cell potential and activating voltage-gated ion channels, as suggested by Beier and colleagues [38][39][40][41][42] . They reported the influx of Ca 2+ ions due to activation of intracellular pathways, can also affect cell membrane fluidity when cells were exposed to millisecond-long infrared laser pulses 38, 41 . Eom et al. 43 reported that astrocytes released intracellular Ca 2+ in response to Au nanorods stimulated with IR light. Earlier studies reported that inhibition due to thermal pulses is likely mediated by potassium channels 19,27,44 . For example, Yoo et al. 19 reported the inhibition of hippocampal neurons in response to illumination of gold nanorods with 785 nm near IR laser. The inhibition was attributed to the involvement of a thermo-sensitive potassium channel -TREK-1. No neural suppression was observed in presence of fluoxetine -a TREK-1 blocker. Rabbitt et al. 44 demonstrated IR stimulation of type II vestibular hair cells ex vivo from mice and afferent neurons in vivo from chinchillas. The inhibition responses were thought to be governed by large conductance Ca 2+ activated potassium channels, so called BK channels. The application of iberiotoxin (IBTX) -a selective blocker for BK channels, eliminated this inhibitory response. Relevant to understanding cellular mechanisms for the present study, SH-SY5Y cells have both TREK-1 and BK channels [45][46][47] . As evident from the above-mentioned studies, there are multiple effects of thermal pulses that could govern the underlying mechanisms of thermal stimulation. Further studies are needed to investigate/understand detailed mechanisms. Along with considering the Hodgkin-Huxley models at macro levels, it will be worthwhile to explore individual channel kinetics and membrane properties at nano-levels with the help of techniques like molecular dynamics simulations.   Nanoparticle-assisted neural modulation is still in the early research stages. Most studies are focused on in vitro proof of concept 14-18, 48, 49 , with very few in vivo experiments 12,16 . In-vivo methods involving nanoparticles raise the issues of toxicity, reliability and bio-compatibility 12,15,16 . Our method of using nanoelectrodes (micro-substrates coated with nanomaterials) which provide a physical buffer between the particles and neural tissue, and optical fibers for delivering laser light locally holds much promise for addressing many of these problems. In-fact, Tran et al. 50 reported that surface-bound Au NPs reduced the cytotoxicity as compared to unbound nanoparticles. They have also used γ-(aminopropyl) triethoxysilane to bind particles to silica surfaces. With a nanoelectrode or similar probe, there is no need to attach nanoparticles to the neurons or alteration of cells at the stimulation interface. In addition, as we reported here, nanoelectrodes can be easily validated for plasmonic responses independently using various methods, such as light-induced photocurrents (few 100 s of nA), fluorescence quenching (emission spectra around 520 nm), plasmonic temperature jumps (pipette resistance method and infrared thermograms). Our nanoelectrode design can be easily adapted for in vivo applications by using nanomaterial-coated biocompatible materials instead of borosilicate glass. There are biocompatible materials which can be used for these prosthetic devices, e.g., polymides, silicon polymers and polymers like polyethylene, polytetrafluoroethylene and polydimethyl siloxane 51,52 . More specifically, there are biocompatible polymers like poly (methyl methacrylate) -(PMMA) and poly (dimethylsiloxane) -(PDMS) which are known to work with silane linkers [53][54][55] . Subsequently, functionalized surfaces can be used to develop Au NPs coated microelectrodes via dip coating methods 56 . It is even possible to use the glass to develop initial stimulating microelectrodes 57 . Implantable silicone would be a good carrier for microelectrodes as it is currently used in many medical applications like cochlear implants, blood vessel, heart valves and prosthetic outer ears 52,58,59 . Light can be delivered via commercially available optical fibers or waveguides. Hence, based on the stimulation results of this report, it is possible to design biocompatible prosthetic devices.
Our future studies will be focused on developing more robust plasmonic stimulation systems by better understanding the biological mechanisms involved, and teasing apart the interactions of nanomaterial/laser parameters for controlling differential neural and cardiac stimulation responses. We will also explore the in vivo applications of plasmonic stimulation with an eye towards the development, initially, of plasmonic-based cochlear implants for deaf people. One of our proposed designs involves a neural cuff or flexible substrate that will employ nanomaterial-coated micro-beads along with focal light-fiber sources. The substrate or cuff has the potential for carrying dozens of electrodes as compared to a maximum of up to 22 electrodes found in current cochlear implants 60 ; with the goal that the new laser stimulation device would stimulate much more discrete groups of ANFs. A more direct method would be adaptation of the currently used electrical stimulation cochlear electrode array with optical fibers replacing the electrical electrodes. In an easy modification of the design, one could structure the electrode to locate polymer-electrode material coated Au NPs very close to the basilar membrane, affording extremely specific stimulation, without thermal damage by the visible light utilized.
Overall, the present report provides fundamental new evidence to support the pursuit of plasmonic stimulation as an alternative approach to conventional electrical neuromodulation, or other emerging modalities, such as IR stimulation. Because the novel technology of the current investigation has capabilities to elicit both inhibitory and excitatory responses, utilizing different stimulation parameters, this type of plasmonic stimulation can be a promising alternative for electrical stimulation paradigms in biological prosthetic implants. As emphasized above, the major disadvantage with IR stimulation is that, along with the target neurons, the IR laser heats up the surrounding tissue as well, which can cause thermal damage and/or unwanted excessive stimulation. By combining visible light, which is minimally absorbed by surrounding tissue and aqueous solutions, with an engineered nanomaterial, we have the potential to achieve a unique, highly localized, yet temporally precise, delivery of energy to the target cells to manipulate their bioelectrical behavior.

Methods
Fabrication of Nanoelectrodes for Cell Stimulation. Au NPs were coated onto glass micropipettes to fabricate the nanoelectrodes for cellular stimulation. Glass micropipettes were pulled using P-97 micropipette puller (Sutter Instruments, Novato, CA). The micropipettes were washed with detergent while heating at 60 °C for 10-15 min, then, washed thoroughly with distilled (DI) water. The micropipettes were then cleaned with a mixture of HCl and methanol (1:1 volume/volume) and washed with DI water, and heated in an oven at 60°C. Next, the micropipettes were immersed in 10% volume/volume solution of γ-(aminopropyl) triethoxysilane (Sigma-Aldrich, St. Louis, MO; APTES, ≥98%) in anhydrous ethanol for 15-20 min. Finally, the pipette tips were washed with ethanol extensively 5x before heating at 120 °C for 3 h. The tips functionalized with amino silane linkers were immersed in Au NPs for 24 h 56 . The final size of micropipettes was 1-2 µm. Au NPs were synthesized using a liquid phase bottom-up approach in which an Au salt solution was reduced with trisodium citrate dihydrate solution. The glassware used for synthesis of Au NPs was thoroughly cleaned with DI water, followed by another thorough cleaning with ethanol. A 20 ml solution of 1 mM gold (III) chloride trihydrate (HAuCl 4 .3H 2 O) (Sigma-Aldrich, ≥99.9%) was boiled on a hot plate in a beaker with continuous stirring. Then, 2-3 ml of a 1% solution of trisodium citrate was added to the boiling HAuCl 4 solution. The solution was boiled until it turned deep red in color, showing the presence of Au NPs 56 . Au NPs were characterized using a UV-Vis spectrophotometer (Perkin Elmer, Waltham, MA; Lambda 35 spectrophotometer) and a transmission electron microscope (FEI Morgagni, Hillsboro, OR). The nanoelectrodes were characterized using a scanning electron microscope (Hitachi SU70 SEM, Krefeld, Germany).
Testing of the Nanoelectrodes. Before proceeding to the in vitro experiments with electrically excitable biological cells (SH-SY5Y and neonatal cardiomyocytes), light-induced photocurrents were recorded when the nanoelectrodes were illuminated with a 532 nm 100 mW green laser (Laser Quantum, Santa Clara, CA; gem 532 high spec OEM laser). The photocurrents were measured using a Gamry reference 600 TM potentiostat and recorded using Gamry Instruments Framework software (Gamry Instruments, Warminster, PA). The instrument was calibrated with a UDC4 dummy cell periodically. A 0.1 M phosphate buffer solution containing 0.05 M EDTA was used as the electrolyte 20 . The nanoelectrode (micropipette coated with Au NPs) and a Pt electrode were used as the two electrode of the electrochemical cell whereas saturated calomel electrode served as the reference electrode. The micropipette (not coated with Au NPs) was used for the control condition. These tests were also carried out using an extracellular solution (ECS) as the electrolyte. The nanoelectrodes were tested for fluorescence quenching by attaching FITC-labeled human serum albumin (HSA) to the nanoelectrode surface and illuminating it with 488 nm blue light under a fluorescence microscope: Olympus BX61W1 upright microscope.

Measurement of Localized Temperature Changes.
Plasmonic temperature elevations on/near the surface of the nanoelectrode were measured using an indirect, pipette resistance method previously developed to measure local temperature changes for infrared stimulation of biological cells 35,61 . A micropipette having a resistance of 5-8 MΩ was filled with ECS and placed near the surface of the nanoelectrode. A 532 nm 100 mW green laser was focused on the tip of the nanoelectrode with the help of an optical fiber having an inside diameter of 50 µm. The experiments were done using a patch clamp system (Multiclamp 700B amplifier and 1440 A data acquisition Molecular Devices, Sunnyvale, CA). A voltage of 20 mV was applied to the circuit and resistance changes were observed when the laser was focused on the nanoelectrode. Control experiments were performed in the absence of the nanoelectrode. The resistance (log R) versus temperature (1/temp. [K]) calibration curves were obtained in response to a 20 mV voltage pulse by allowing the hot ECS solution (approximately 55 °C starting point) to cool down to room temperature in a Petri dish. The temperature was continuously monitored using a thermistor (monitor thermistor of temperature controller TC-324 unit from Warner Instruments, Hamden, CT). As temperature goes down, corresponding resistance was measured. Temperature variation was measured as functions of laser power and distance from the nanoelectrode. Plasmonic temperature increases were also confirmed using an infrared thermal camera (FLIR T420 thermal camera with detector spectral range 7.5 to 13.0 µm).
Cell Culture. Neuroblastoma Cells. Neuroblastoma, SH-SY5Y (ATCC ® CRL-2266) can be differentiated into neurons in presence of retinoic acid. The cell lines were cultured in a mixture of DMEM and F12 (1:1 volume/ volume) media containing 10% FBS and 1% penstrep. Cells were cultured at 37 °C with 5% CO 2 . After 48 h of plating, the media was replaced with neurobasal media containing B27, GlutaMAX and penstrep as the supplement. 10-20 µM of all-trans-retinoic acid (ATRA) was added into the media to facilitate the differentiation. The media was changed every 48-72 hr 62,63 .
Neonatal Cardiomyocytes. The heart tissue was provided by the laboratory of Prof. Cheryl Kirstein, Department of Psychology, University of South Florida, Tampa, FL. Neonatal cardiomyocytes were obtained from 2-3 day old Sprague Dawley rat pups. 8-10 pups were decapitated and their hearts were removed. The hearts were transferred to ice cold PBS containing 20 mM glucose. The atria were removed using a small scissors to expose the ventricle cardiomyocytes, which were subsequently, minced into small pieces. For digestion, the collagenase solution was prepared by adding collagenase type II (1 mg/ml) and 1% bovine serum albumin (BSA) into phosphate buffered saline (PBS) having 20 mM glucose. The minced ventricle parts were digested using collagenase solution. The cells were cultured in M199 media, having 5% FBS, 10% horse serum and 0.1% penstrep. The cells were incubated at 37 °C with 5% CO 2 . The cardiomyocytes showed spontaneous beating in days 1-2 64 . All the animal protocols were approved by University of South Florida Institutional Animal Care and Use Committee (IACUC) and are consistent with US Federal and NIH guidelines.
In vitro Cell Stimulation. In vitro stimulation experiments were done using a patch clamp system in the whole cell current clamp configuration. These physiological experiments were done using a Multiclamp 700B amplifier and Digidata 1440 A data acquisition system (Molecular Devices, Sunnyvale, CA). Experiments were carried out at room temperature. Patch pipettes were pulled using a P-97 micropipette puller (Sutter Instruments, Novato, CA) and their capacitance was corrected using Multiclamp software. After the giga-Ω seal on the cell membrane with the pipette, the whole cell configuration was achieved by applying suction pressure to the patch pipette using a 1 ml syringe. Subsequently, the amplifier (Multiclamp 700B, Molecular Devices, Sunnyvale, CA) was switched to the current clamp mode. Then, the nanoelectrode was placed just next (within 2 µm) to the patch cell. A 532 nm green laser was focused on the tip of the nanoelectrode using an optical fiber having inside diameter of 50 µm. Two 532 nm lasers were used for this study -A Gem 532 nm green laser from Laser Quantum (Santa Clara, CA) and OBIS 532 nm laser from Coherent (Santa Clara, CA). The lasers were controlled using a function generator (AFG 320 Sony Tektronix, Beaverton, OR). The power was varied by altering the power of input voltages to the laser and the lasers were calibrated on a monthly basis using a power meter (Coherent, Santa Clara, CA; FieldMAX light power meter). The cell membrane potential was adjusted by applying a holding under current clamp mode. In this way, we sought to determine the effects of laser stimulation on evoked membrane potentials. For 10 ms, 100 mW laser pulse stimulation experiments ( Fig. 4a and b), a series of holding currents were applied to do the paired analysis of evoked potentials at different baseline membrane potentials. For inhibition experiments, laser pulses were applied simultaneously with electrical current pulses under current clamp mode and membrane potential responses were recorded. Data acquisition and analysis were performed with pClamp & Clampfit (Molecular Devices, Sunnyvale, CA) and GraphPad Prism (La Jolla, CA) software packages.