Figure 6 | Scientific Reports

Figure 6

From: Selective cytotoxicity of a novel immunotoxin based on pulchellin A chain for cells expressing HIV envelope

Figure 6

Comparing the cytotoxicity and targeting of ITs by direct cytotoxicity assay. (A) The cytotoxicity of 924-based ITs was assayed by using persistently-infected H9/NL4-3 cells. 924-RAC appears more cytotoxic than 924-PAC. ITs did not have cytotoxicity on T-cell lymphoma (H9) cells, signifying ITs have specific targeting. (B) The cytotoxicity of 7B2-based ITs to Env-transfected 293 T cells (293 T/92UG) in the presence or absence of sCD4 (anti-gp120) was assayed. The results show that 7B2-RAC and 7B2-PAC had equivalent cytotoxicity. The presence of soluble CD4 (300 ng/ml) had a significant enhance on the cytotoxicity of ITs. (C) The cytotoxicity of ITs was compared before and after labeling in the presence of sCD4 (300 ng/ml). Results show mean and standard error of triplicate samples. Results are representative of three individual experiments. Where no error bars are visible they are obscured by the symbol.