Figure 3 | Scientific Reports

Figure 3

From: Selective cytotoxicity of a novel immunotoxin based on pulchellin A chain for cells expressing HIV envelope

Figure 3

Binding ability of 7B2 based-ITs to gp41 antigen by using ELISA and flow cytometry. (A) ELISA plates coated with gp41 Ag, as a peptide representing 7B2′s epitope. Binding of the 7B2 based-ITs is detected by AP-conjugated goat anti-human IgG. Results are representative of means of triplicate values with three individual experiments. (B) Using FITC-secondary immunofluorescence, we detect binding of ITs to Env-transfected 293 T cells in the presence (darker line) or absence (lighter line) of sCD4 (CD4-183). Isotype control is shown as red shaded histogram. (C) IT binding to 293 T cells and transfected 293 T cells with 92UG037.8 gp160 (293 T/92UG) was detected by using FITC-secondary immunofluorescence. Results are representative of at least three independent experiments. (D) The flow cytometry histogram of Alexa488-labeled ITs by using Env-transfected 293 T cells incubated in PBS + 1% BSA + sodium azide (0.2%). Results are representative of three individual experiments. Isotype control (chimeric RAC18-alexa488) is shown as red shaded histogram.