Figure 2 | Scientific Reports

Figure 2

From: Selective cytotoxicity of a novel immunotoxin based on pulchellin A chain for cells expressing HIV envelope

Figure 2

924 based-ITs bind to recombinant antigens and native antigens on the HIV infected cells. (A) ELISA plates are coated with a synthetic V3 loop peptide, recombinant gp120 and gp41 peptide as a control ligand. The results show the binding of 924 MAb and 924-ICs to the either gp120 or V3 peptide, but not the unrelated isotype controls. (B) ELISA plates are coated with recombinant RAC, PAC and recombinant gp120 as a control ligand. The results demonstrate the binding of anti-ricin A chain MAb (RAC18) to rPAC and rRAC, but not the unrelated isotype control. In panels (A and B) the Ab binding is detected with AP-conjugated goat anti-mouse IgG. Where no error bars are visible they are obscured by the symbol. Results are representative of means of duplicate values with at least three different assays (varying by Ab, ITs, or Ag tested). (C) Flow cytometry histograms for binding of 924 MAb and 924 based-ITs to uninfected H9 cells, as control, and persistently-infected H9/NL4-3 cells. Binding was detected with Alexa488 conjugated goat anti-mouse IgG. On the right, IT binding to H9/NL4-3 cells is plotted as median fluorescence versus IT concentration. The results are representative of three independent experiments. Isotype control is shown as red shaded histogram.