Figure 1 | Scientific Reports

Figure 1

From: Selective cytotoxicity of a novel immunotoxin based on pulchellin A chain for cells expressing HIV envelope

Figure 1

Characterization of immunoconjugates (ICs). (A) The table shows components of ICs with different concentrations of LC-SPDP biolinker. The final products include 10 ICs as well as 2 conjugates with irrelevant MAb, as control. (B) Microcapillary electrophoresis of reduced and non-reduced ICs. Results are displayed in the familiar format of a coumassie stained gels. Size standards are indicated on the side of each “gel”. PAC, RAC and light chain of 924 MAb represent the same molecular size on the gel. The star symbol (*) means under reducing conditions by using 2-Mercaptoethanol. The ICs represent the conjugation with 40-fold molar excess of LC-SPDP biolinker. In Figure S1, microcapillary electrophoresis of conjugates with different concentration of biolinker are demonstrated. (C) Electropherogram of 924-PAC IC, after passing from Ultra-100K centrifugal filter, demonstrates the negligible presence of free MAb as well as MAbs conjugated with 1, 2, 3 or even 4 PAC. (D) Peak table of RAC and PAC ICs show the percentage of MAbs conjugated with A chains.