Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Heterogenous oceanic redox conditions through the Ediacaran-Cambrian boundary limited the metazoan zonation


Recent studies have enhanced our understanding of the linkage of oxygenation and metazoan evolution in Early Cambrian time. However, little of this work has addressed the apparent lag of animal diversification and atmospheric oxygenation during this critical period of Earth history. This study utilizes the geochemical proxy and N isotope record of the Ediacaran–Cambrian boundary preserved in intra-shelf basin, slope, and slope basin deposits of the Yangtze Sea to assess the ocean redox state during the Early Cambrian metazoan radiation. Though ferruginous conditions appear to have prevailed through the water column during this time, episodes of local bottom-water anoxia extending into the photic-zone impacted the slope belt of the basin. Heterogenous oceanic redox conditions are expressed by trace element concentrations and Fe speciation, and spatial variation of N isotopes. We propose that the coupling of ocean chemistry and Early Cambrian animal diversification was not a simple cause-and-effect relationship, but rather a complex interaction. Specifically, it is likely that animal diversification expanded not only temporally but also spatially from the shallow shelf to deep-water environments in tandem with progressive oxygenation of the extensive continental margin.


The Cambrian explosion of diverse bilaterian clades is considered to have proceeded in three phases preserved in the fossil record: (1) the first phase is recognized by the appearance of basal metazoan phyla in Late Ediacaran time; (2) the second phase begins with the first occurrence of biomineralization of plates, spines and shells, and is recorded by the widespread occurrence of small shelly fossils (SSFs); (3) the final, perhaps the most profound, phase is defined by the emergence of the three supraphylogenetic clades during Cambrian stage 31. Though these biological events have been attributed to environmental modifications, notably oxygenation of the global ocean, specifics of the relationship between the emergence and diversification of metazoans and atmospheric-oceanic oxygenation remain unresolved. Large-scale investigations have focused on the nature of geochemical constraints on atmospheric oxygen levels through geological time2,3,4,5,6,7 and (minimum) oxygen requirements for the stimulation and maintenance of metazoan evolution8,9,10,11. Conflicting conclusions of the necessity of oxygenation to the biological evolution of metazoans have been reported. Indeed, some have argued that increasing atmospheric oxygen levels might not have triggered the appearance of metazoans12. However, increasing concentrations of dissolved oxygen would have been required to sustain the subsequent diversification of metazoans and the establishment of a Phanerozoic-type ecosystem. Other studies have postulated that the activity of benthic faunas improved the chemical and physical conditions of their habitats by bioturbation and related bioirregation since the Fortunian stage13. Finally, although inferred atmospheric oxygen levels suggest that the global ocean may have been oxygenated by Late Ediacaran time, anoxic (perhaps intermittently euxinic) waters appear to have persisted in some areas of the Early Cambrian ocean.

A variety of geochemical approaches have been employed to characterize oceanic redox conditions during the Ediacaran–Cambrian boundary interval. Increasing δ98/95Mo from +1.7‰ at ~540 Ma to +2.3‰ (similar to modern seawater composition) by ~520 Ma suggests that bottom waters were fully oxygenated by Cambrian stage 314. Fe speciation and S isotope data reflect the existence of a mostly ferruginous water column above a zone of limited bacterial sulfate reduction (BSR) in continental shelf and deep waters until Cambrian stage 3 in the Yangtze Sea15,16,17. Benthic redox conditions likely fluctuated frequently in intra-shelf basin areas of the Yangtze Sea as suggested by documented authigenic Mo-U co-variation18. Cerium anomalies documented from phosphate nodules recovered from basal Cambrian strata are consistent formation beneath an oxygenated water column19, yet the existence of photic-zone anoxia is suggested by largely negative sedimentary N isotope values described from Ediacaran-Cambrian deposits of the Yangtze platform20, 21. Thus, various geochemical proxies, some of them conflicting, suggest that redox history of Yangtze Sea was quite debatable at the time of the Cambrian explosion.

Herein, we address water column geochemistry of the Yangtze Sea during the Ediacaran-Cambrian transition using a combination of independent redox proxies, including N isotopes, trace element concentrations (mainly Mo and U), and Fe speciation. Application of this approach to the investigated sections, which were selected based on their inferred paleoceanographic positions within the Yangtze Sea (Fig. 1), elucidates spatial and temporal heterogeneity of ocean redox states. Longer-term geochemical trends are also addressed as a means of assessing the evolution of ocean chemistry through geologic time. A principal goal of the present study, then, is an improved understanding of the interaction of ocean redox state and the Cambrian metazoan explosion.

Figure 1

Paleoenvironmental map of the South China Block in the Early Cambrian (after ref. 18, with permission from Elsevier), showing locations of investigated sections. The figure was created by the author and co-authors, with mainly MapGIS 9.0 ( constraining the geographic locations and CorelDRAW × 4 ( further enhancing this illustration.


Sedimentary N isotope records

Reconstruction of the N cycle is essential to exploring the potential links between ocean chemistry and the marine ecosystem22,23,24,25. Sedimentary δ15N values of investigated samples range from −7.5‰ to +5.9‰, showing the greatest variation for all studied sections near the Ediacaran-Cambrian boundary (Fig. 2; refer to supplementary text for the stratigraphic and sedimentological context of the investigated intervals). The Zhongnan section displays consistently positive δ15N values with the exception of a single negative value obtained from a black shale sample (Fig. 2). The first interval in the Zhongnan section, which includes phosphate-rich deposits as well as intercalated chert and shale, is featured by values between +0.4‰ and 2.4‰, increasing slightly up-section (Fig. 2). Interval 2 is recognized by a sharp δ15N decrease of ~9.4‰, followed by a rapid return to positive values persisting into the third interval (Fig. 2). A subtle positive excursion appears at the onset of interval 3 that passes upward from a value of 0‰ near the Ni-Mo sulfide layer (Fig. 2).

Figure 2

δ15N and redox-sensitive trace-metal concentrations for those investigated sections. Colored zones for N isotope profiles are used to show the transition from strong N-fixation to photic-zone anoxia. Three stratigraphic intervals are based on biostratigraphy and chemostratigraphy. See supplementary text for dividing details and Fig. S2 for lithology symbols. DY Fm. = Dengying Formation.

Slope deposits of the Nangao section exhibit a δ15N profile different with that of the Zhongnan section. A weak positive excursion is observed near the bottom of interval 1 (Fig. 2). An overlying negative excursion in interval 2 is much smaller than its equivalent event in the Zhongnan section (Fig. 2). A much stronger negative excursion is documented just below the boundary of interval 2 and 3, immediately below the sulfide ore layer (Fig. 2). Above this, the δ15N profile displays a broad but subtle positive excursion of approximately 1.7‰ through interval 3 (Fig. 2). The δ15N profile of the Longbizui section displays generally lower values relative to the Zhongnan sections (Fig. 2). A positive excursion of ~5.9‰ occurs in the Liuchapo Formation of interval 1 (Fig. 2). A sharp negative shift by ~5.2‰ is observed at the beginning of interval 2. Further, negative δ15N values are concentrated in black shale in the interval 3 above the Ni-Mo sulfide layer20, 21.

Principal biogeochemical processes that influence N isotope composition include N-fixation by diazotrophic cyanobacteria, denitrification in the water column or within sediment and anaerobic ammonium oxidation (anammox)24. Assimilation of N2 into organic matter by N-fixation is accompanied by fractionation of as much as −4‰26. Denitrification of NO3− to N2 is accompanied by15N-enrichment of the remaining NO3− pool. Additional processes affecting N isotope composition include expansion of the oxygen-minimum zone and shoaling of the O2/H2S chemocline into the photic zone27. In each scenario, increased utilization of 15N-depleted ammonium by primary producers (dominantly eukaryotes) results in a reduction of δ15N values of accumulating sediment. Especially, marine sediments deposited during the OAEs may record striking 15N-depletion, with δ15N values commonly <−2‰ interpreted to be an expression of strongly reducing water conditions28, 29. Sharply negative δ15N deviations (by 2–4‰) from normal marine N cycling, then, can be considered as the evidence of photic-zone anoxia perhaps induced by a chemocline upward excursion29.

The N isotope composition of sedimentary organic matter can also be altered by diagenetic processes24, 29. The diagenetic loss of N from organic matter would be expected to yield C/N atomic ratios in excess of the 6.6 value of the Redfield ratio. Though the average C/N ratio of studied samples reaches 43.5, the weak correlation of δ15N and C/N (R2 = 0.0284) is not consistent with an interpretation involving strong diagenetic alteration of organic matter. Indeed, the weakly negative correlation (R2 = 0.3924) of δ15N and C/N values of the Nangao section suggests minor effect of diagenetic alteration on N isotopes (Fig. 3A). Further, given that the maximum isotopic deviation that can be ascribed to the selective removal of protein-N is generally no more than 2‰29, it is likely that the δ15N profile of the studied sections have been only minimally affected by diagenesis. It is noteworthy that N isotopes and C/N values described from modern and ancient shale successions, some of which accumulated during oceanic anoxia events (OAEs) are analogical to those of the present study (Fig. 3A). Thus the negative δ15N excursions documented from the sections spanning the Ediacaran-Cambrian boundary probably reflect some degree of modification of nitrogen cycling related to the shoaling of chemocline into the photic zone. The limited occurrences of negative δ15 N tend to argue against the establishment of photic zone euxinia. Instead, it is more likely that it was the expansion of anoxic bottom water into the photic zone that resulted in the consumption of 15N-depleted ammonium resulting in the negative δ15N excursions documented in sections along the slope belt, including the Nangao section. However, the possibility that photic zone euxinia occurred perhaps intermittently can’t be ruled out and should be addressed by further study of these deposits.

Figure 3

(A) Scatter plot of δ15N data and atomic C/N ratios from diverse depositional environments represented in various sections comprising Yangtze Sea deposits (refs 20, 21 and this study). The gray shade represents the range for those samples deposited during the OAEs29. (B) Comparisons of δ15N values recorded by marine sediments from the early-Cambrian Yangtze Sea and those from periods of widespread anoxia and OAEs (Data are sourced from refs 28 and 29). The gray box represents the range of values excluding outliers, with the line red/white in the box indicating mean values.

Details of N cycling preserved in a sedimentary deposit may reflect spatial variation of oceanic redox conditions from shallow to deep waters (Fig. 3B). Elevated δ15N values of approximately 0‰ recorded in the Zhongnan section may tell of episodes of N cycling in oxygenated surface water or slight denitrification within the water column below the oxic-suboxic boundary, as the stratified redox structure allows. However, the generally negative δ15N values common to slope deposits of the Nangao section could not have resulted from normal N cycling. Rather, such low values have originated from primary producers utilizing 15N-depleted NH4 +, a process consistent with what is known of oceanic redox structure associated with the OAEs26, 28. The nearly consistent δ15N values of approximately −1‰ through interval 3 are suggestive of a redox-stratified ocean. As a redox-stratified ocean, the negative δ15N prevailed around −1‰ without significant excursions through the interval 3. Spatial variation of δ15N values appears inconsistent with widespread N cycling in late-Neoproterozoic ocean25, 26. Instead, anoxia may have been localized along the continental margin of Yangtze Sea, to some extent, lagging regional progressive oceanic oxygenation.

Enrichments of redox-sensitive metals

Redox-sensitive metals, including Mo, U, V and Cr, are generally enriched under reducing bottom-water conditions30. The Zhongnan section exhibits variable, but enriched concentrations of these elements (Table S1 and Fig. 2). Mo concentrations range from 1.8 ppm in dolomite to 735 ppm associated with the sulfide layer. Mo is strongly enriched in the black shale, averaging approximately 300 ppm. Samples recovered from the basal Cambrian interval, including chert, are moderately enriched in Mo (19.2 ppm – 188 ppm). Uranium displays variable concentrations through the section from ~300 ppm in phosphate-rich deposits at the base of the Cambrian interval to ~30 ppm in overlying chert-bearing deposits, to 100 ppm in black shale higher in the succession (Fig. 2). V and Cr are strongly enriched in, and display generally sympathetic co-variance through, the Zhongnan section. However, V attains its highest value (3480 ppm) and Cr its lowest concentration (213 ppm) immediately below the Ni-Mo sulfide layer. Black shale samples of the Nangao section display lower enrichments of Mo (maximum = 149 ppm; average = 81 ppm) and U (maximum = 85 ppm; average = 37 ppm) than those of the Zhongnan section (Fig. 2). Those samples collected proximal to the Ni-Mo sulfide layer exhibit high concentrations of Mo and U (Fig. 2). It is noteworthy that the phosphate-enriched deposits of the Nangao section contain less U (<100 ppm) than equivalent rocks of the Zhongnan section (Fig. 2). The Longbizui section exhibits relatively low concentrations of Mo and U, yet higher contents of V and Cr. Chert samples of the Liuchapo Formation yield moderate concentrations of Mo (4–20 ppm) and low U (<3 ppm) (Fig. 2). Mo and U increase through the lower part of the Niutitang Formation of the Longbizui section, attaining maximum values (808 ppm and 331 pm, respectively) near the sulfide layer. V (maximum = 10,389 ppm) and Cr (maximum = 2,034 ppm) are strongly enriched through the interval of intercalated chert and shale of the Niutitang Formation.

Given that thiomolybdate formation requires free H2S31, 32, enhanced authigenic U uptake relative to Mo is to be expected under microbial-mediated conditions of higher redox potential, including suboxic or dysoxic conditions33. Organic-rich deposits of the studied sections display variable levels of U and Mo enrichment, suggesting anoxic to euxinic bottom water conditions (Fig. 4). Mo/U ratios of the Zhongnan section illustrate the greatest degree of scatter, varying from <0.3 × SW to >3 × SW, suggesting highly variable benthic redox conditions. Algeo and Tribovillard (2009)33 have interpreted MoEF and UEF trends in terms of several modern ocean systems. According to this classification, the intra-shelf basin within which deposits of the Zhongnan section accumulated was likely weakly restricted18. Weak basin restriction may have led to stratification of the water column and establishment of anoxic or euxinic bottom-water conditions as suggested by Mo and U enrichments and negative δ15N excursions. This interpretation is consistent with previous studies that document negative 98/95Mo excursions documented from deposits immediately above the Ediacaran-Cambrian boundary tell of a restricted water column depleted of Mo34, 35. Shale deposits that accumulated in deep-water basin, the shale yield also high enrichments of Mo, U and redox-sensitive metals, indicating anoxic to euxinic bottom-water conditions even without hard evidences recording the existence of H2S in the water column. But the Mo-U covariation trends for deep-water sections display narrower slope ranges than that of Zhongnan sections, suggesting relatively stable bottom-water redox conditions (Fig. 4).

Figure 4

Plots to show Mo (EF) versus U (EF) for investigated sections. The solid diagonal line shows the seawater Mo/U molar ratio (1 × SW) and the dashed lines multiples thereof (3 × SW, 0.3 × SW). The olive-green field represents the “particulate shuttle” trend, characteristic of depositional systems in which intense redox cycling of metal, especially Mn oxyhydroxides occurs33.

Enrichments of other redox-sensitive metals, including V and Cr, vary synchronously with Mo and U. Most Cr/Ti ratios of the investigated sediments are much greater than the crustal value of 0.0202 (calculated from modern crustal concentrations), suggesting that Cr enrichment principally by scavenging of particle reactive Cr (III) phases under strongly reducing conditions. Relatively low Cr/Ti ratios documented from the upper Niutitang Formation (interval 3) reflects less reducing conditions. Accelerated uptake of redox-sensitive metals and the coeval development of strong negative excursions in each of the investigated sections suggest the establishment of widespread reducing conditions. However, anoxic conditions appear to have persisted in slope regions into interval 3 as recorded by elevated Cr/Ti ratios and enrichment of Mo, U and V (Fig. 2). While the V concentrations express interestingly similar stratigraphic trends with Cr/Ti ratios, providing more evidences to shed light on the redox history.

The documented temporal association of stable N isotopic excursions and periods of redox-sensitive trace metal enrichment is consistent with what is known of the oceanic redox structure, but its timing may vary across the basin. Interestingly, the onset of negative δ15N excursions in Zhongnan and Longbizui sections appear to have occurred somewhat earlier than strong enrichment of redox-sensitive metals (especially Mo) associated with intervals 1 and 2. The Fe proxy indicates that the global ocean was dominated by ferrugenous water through the late Ediacaran to interval 136. Accelerated upwelling at this time enhanced surface water productivity18 thereby increasing the flux of organic matter to the ocean floor, which further reduced bottom-water conditions. The shoaling of anoxic water mass into the photic zone caused primary producers to consume15N-depleted NH4 + resulting in the distinctly negative δ15N values documented from the studied deposits. It appears that enrichment of redox-sensitive trace metals lagged behind the isotopic response, gradually increasing as euxinic bottom water conditions were established. As modern oceans indicate, the residence times of those geochemical indices are ~3 kyr for N isotope, ~780 kyr for Mo and ~480 kyr for U, respectively. Thus a reason for the lag is proposed that isotopic evidences respond to oceanic changes quicker than trace metals. Probably, global ocean began to yield the oceanic anoxia earlier than the local ocean that experienced reducing conditions and consequent accumulation of redox-sensitive metals. However, this lag is not recognized in the slope belt, including the Nangao section. It is plausible that a rapidly rising sea level caused anoxic bottom water to shoal into the photic-zone producing the negative isotope excursion. Enrichment of trace metals appears to have accelerated across the basin at this time18, 34. Following this event and the related negative δ15N excursion, N cycling in the intra-shelf basin (Zhongnan section) returned to a more normal state with the δ15N values varying between 0‰ and 2‰. However, euxinic water persisted in the slope and deep-water basin, with unscheduled shoaling, as indicated by negative δ15N values (mostly <−1‰) and high concentrations of Mo in sediments. Despite the branching in time probably resulted, both the N cycling and trace-metal enrichment respond to the fluctuating oceanic redox state and reflect evidently in their sedimentary evidences.


Results of the present study suggest that areas of the Yangtze Sea experienced rapid fluctuations of oceanic redox conditions following the Cambrian explosion that may reflect lower than expected oceanic and atmospheric oxygen levels at this time. The Ediacaran biota may tell of increased atmospheric oxygen content and related widespread oceanic oxygenation following the Gaskiers glaciation (580 Ma). However, these conditions may have persisted for only several million years before the ocean, except for the mixed surface layer, returned to anoxic and ferruginous conditions that persisted well into the Early Cambrian36. During this time, though, the water column of the shallow shelf remained oxygenated, accumulating widespread carbonate sediment as exemplified by the Xiaotan section in the eastern Yunnan province and studied sections in the Three Gorge Area (Fig. 1)20, 37. Intra-shelf basins, including the depositional site of the Zhongnan section, became anoxic or even euxinic, as suggested by the present and previous investigations18, 35. Further, the presence of largely negative δ15N excursions as well as elevated Mo/U and Fepy/FeHR ratios observed in the studied sections may reflect local establishment of photic zone anoxia. Water column conditions of the slope area of the basin seaward represented by the Nangao section appear to have remained strongly reducing when the water column got improved elsewhere. Clearly, δ15N profiles of the deeper water Nangao and Longbizui sections display more consistently negative values than are documented from the intra-shelf Zhongnan section (Fig. 2) suggesting that these deposits experienced a protracted period of strongly reducing conditions. It should be noted that, however, iron speciation data from previous studies suggest that bottom–water conditions of the slope-to-basin region of the basin was seldom euxinic (Fig. 5). In summary, the commonly ferrugenous sub-photic water column of the Yangtze Sea margin experienced local anoxia or euxinia in intra-shelf basin and upper slope regions of the basin. The postulated redox state of the Yangtze Sea may have persisted until the ocean was fully oxygenated17, 37. Such diverse paleo-marine environmental conditions characterized the continental shelf of the Yangtze Sea in the face of the Cambrian explosion.

Figure 5

Fe speciation diagram for Cambrian samples from various depositional environments. Data are compiled from refs 14,15,16, 35 and 41.

An overview of geochemical evidences from secular and global perspectives, may tell of slightly different evolution history of atmosphere-ocean chemistry (Fig. 6). The sharp rise of oxygen levels, featured by great oxidation events (GOEs) during the Proterzoic, are also recorded by the highly increasing access of trace metals like Mo and U in the ocean4, 5. Recent studies24, 25, including results of the present investigation, have presented secular coupling records of N isotopes and inferred atmospheric oxygen levels for the Ediacaran-Cambrian boundary (Fig. 6A). Strong enrichment of Mo and U and negative N isotope trends in deposits that accumulated along the Yangtze Platform suggest that anoxic conditions existed in deep waters until Stage 3. Secular N isotope records provide evidence for progressively enhanced denitrification in the water column (highest to 8‰). Isotopic trends of diminishing values can be related to normal marine N cycling in oxygenated shallow water. Alternatively, in deep waters, reductions of δ15N may reflect accumulation of sediment under strongly reducing conditions, an interpretation buttressed by strong concentrations of redox-sensitive trace metals, including Mo and U (Fig. 6B). Though the existence of euxinic bottom-water conditions are challenged by published Fe speciation data, the establishment of photic zone anoxia along the slope on the eve of extensive metazoan radiation linked to oceanic oxygenation requires much more attention.

Figure 6

Integrated compilation of diverse geochemical indices with the biodiversity data. (A) Geochemical indices through geologic time. Mo concentrations were picked from refs 4 and 14, and time-averaged U concentrations were selected from the data of ref. 5, including data of this investigation. The composite curve of secular trends of δ15N are according to ref. 24. The curve of atmospheric oxygen levels relative to present is according to refs 2, 3 and 7. (B) Concentrations of Mo and U and MoEF/UEF through time (541–515 Ma); compilation of δ15N values from a range of depositional environments in the Early Cambrian Yangtze Sea (541–515 Ma). N isotopes are compiled from refs 20, 21 and this study. (C) Comparative bioturbation index and ichnodiversity data (referred to ref. 13); animal diversity from ref. 42 and SSF diversity data from ref. 43. The ages of glacial and green-house intervals are according to ref. 24. The white arrows in (B) represent the overall variation tendency of δ15N values.

The Cambrian explosion was accompanied by increasing ichnodiversity that appears to have been unrelated to the appearance of body-fossil assemblages, including the Chengjiang Fauna13. Further, the establishment of suspension-feeding communities during Cambrian stage 3 is believed to have triggered the subsequent evolution of large metazoans and played a critical role in nutrient cycling. Increasing levels of bioturbation associated with the Cambrian explosion (Fig. 6C) would likely have resulted in more robust bioirregation and related oxygenation of benthic habitats. However, geochemical evidence of the present study suggest that deep waters, even on the shelf margin, remained anoxic (ferruginous) in the face of strengthening bioturbation38. Alternatively, the rise of body-size of animals during Early Cambrian time has been related to increasing ambient atmospheric oxygen concentrations8 and the consequent establishment of an enhanced early animal food web9. Thus, the early development of a Phanerozoic-type ecosystem should have oxygenated the ocean during the Cambrian stage 3. But oceanic oxygenation appears to have lagged in slope regions of the basin relative to the oxygenated shallow-water environments. The Cambrian explosion may have entailed not only rapid faunal diversification and ecosystem modification, but an ecological expansion from the oxygenated shelf to the anoxic deep-water realm as bottom-water oxygen levels increased. The described relationship of animals and environment comprises a complicated feedback, rather than a simple cause and effect link. Future work should focus on the association of species expansion and bioturbation with global spatial-temporal variability in ocean chemistry.


The sampling strategy was designed to provide high resolution coverage of the Ediacaran-Cambrian boundary to Cambrian stage 3. Dozens of samples were analyzed for nitrogen isotopes, trace element concentrations and TOC (Table 1). Samples were selected from fresh exposures free of nodules. Analytical work was carried out at the Beijing Research Institute of Uranium Geology. All samples were crushed to powder (<200 mesh) before processing. TOC values were obtained using an ELTRA CS580-A carbon-sulfur analyzer and reported as weight percent.

Samples selected for N analysis were first analyzed for the total nitrogen (TN) content. Only those samples in which TN > 0.012 mg (calculated weight) were deemed capable of providing reliable nitrogen isotope results. These samples were analyzed on a Finnigan MAT-253 stable isotope mass spectrometer. Nitrogen isotope (δ15N) values were calculated according to the formula, δ15N = ((15N/14Nsample)/(15N/14Nair) − 1) * 1000‰.

Samples analyzed for N isotopes were also analyzed for major and trace element concentrations. Major element analysis was carried out using an Axios mAX X-ray fluorescence spectrometer with results reported as weight percent of oxides. Trace elements were measured by on ELEMENT XR ICP-MS instrument. Analytical uncertainties for analyzed elements was less than 5%. Enrichment factors (EF) were calculated as per the following; X (EF) = (X/Al)sample/(X/Al)PAAS, where X is the concentration of an element of interest and PAAS refers to post-Archean average shale compositions of Taylor and McLennan39. Authigenic trace element concentrations were calculated as follows: Xauth = Xsample − (X/Al)detr * Alsample, where detrital element concentrations are those of the average upper continental crust of McLennan40.


  1. 1.

    Shu, D., Isozaki, Y., Zhang, X., Han, J. & Maruyama, S. Birth and early evolution of metazoans. Gondwana Res. 25, 884–895 (2014).

    Article  Google Scholar 

  2. 2.

    Berner, R. A. Isotope Fractionation and Atmospheric Oxygen: Implications for Phanerozoic O2 Evolution. Science 287, 1630–1633 (2000).

    ADS  CAS  Article  PubMed  Google Scholar 

  3. 3.

    Berner, R. A. GEOCARBSULF: A combined model for Phanerozoic atmospheric O2 and CO2. Geochim. Cosmochim. Acta 70, 5653–5664 (2006).

    ADS  CAS  Article  Google Scholar 

  4. 4.

    Reinhard, C. T. et al. Proterozoic ocean redox and biogeochemical stasis. Proc. Natl. Acad. Sci. USA 110, 5357–5362 (2013).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  5. 5.

    Partin, C. A. et al. Large-scale fluctuations in Precambrian atmospheric and oceanic oxygen levels from the record of U in shales. Earth Planet. Sci. Lett. 369–370, 284–293 (2013).

    Article  Google Scholar 

  6. 6.

    Planavsky, N. J. et al. Low mid-Proterozoic atmospheric oxygen levels and the delayed rise of animals. Science 346, 635–638 (2014).

    ADS  CAS  Article  PubMed  Google Scholar 

  7. 7.

    Lyons, T. W., Reinhard, C. T. & Planavsky, N. J. The rise of oxygen in Earth’s early ocean and atmosphere. Nature 506, 307–315 (2014).

    ADS  CAS  Article  PubMed  Google Scholar 

  8. 8.

    Payne, J. L. et al. Two-phase increase in the maximum size of life over 3.5 billion years reflects biological innovation and environmental opportunity. Proc. Natl. Acad. Sci. USA 106, 24–27 (2009).

    ADS  CAS  Article  PubMed  Google Scholar 

  9. 9.

    Sperling, E. A. et al. Oxygen, ecology, and the Cambrian radiation of animals. Proc. Natl. Acad. Sci. USA 110, 13446–13451 (2013).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  10. 10.

    Mills, D. B. et al. Oxygen requirements of the earliest animals. Natl. Acad. Sci. USA 111, 4168–4172 (2014).

    ADS  CAS  Article  Google Scholar 

  11. 11.

    Sperling, E. A. et al. Statistical analysis of iron geochemical data suggests limited late Proterozoic oxygenation. Nature 523, 451–454 (2015).

    ADS  CAS  Article  PubMed  Google Scholar 

  12. 12.

    Mills, D. B. & Canfield, D. E. Oxygen and animal evolution: did a rise of atmospheric oxygen “trigger” the origin of animals? Bioessays 36, 1145–1155 (2014).

    CAS  Article  PubMed  Google Scholar 

  13. 13.

    Mangano, M. G. & Buatois, L. A. Decoupling of body-plan diversification and ecological structuring during the Ediacaran-Cambrian transition: evolutionary and geobiological feedbacks. Proc. Biol. Sci. 281, 20140038 (2014).

    Article  PubMed  PubMed Central  Google Scholar 

  14. 14.

    Chen, X. et al. Rise to modern levels of ocean oxygenation coincided with the Cambrian radiation of animals. Nat. Commun. 6, 7142 (2015).

    CAS  Article  PubMed  PubMed Central  Google Scholar 

  15. 15.

    Canfield, D. E. et al. Ferruginous conditions dominated later neoproterozoic deep-water chemistry. Science 321, 949–952 (2008).

    ADS  CAS  Article  PubMed  Google Scholar 

  16. 16.

    Wang, J., Chen, D., Yan, D., Wei, H. & Xiang, L. Evolution from an anoxic to oxic deep ocean during the Ediacaran–Cambrian transition and implications for bioradiation. Chem. Geol. 306–307, 129–138 (2012).

    Article  Google Scholar 

  17. 17.

    Feng, L., Li, C., Huang, J., Chang, H. & Chu, X. A sulfate control on marine mid-depth euxinia on the early Cambrian (ca. 529–521Ma) Yangtze platform, South China. Precambr. Res. 246, 123–133 (2014).

    ADS  CAS  Article  Google Scholar 

  18. 18.

    Zhang, J., Fan, T., Algeo, T. J., Li, Y. & Zhang, J. Paleo-marine environments of the Early Cambrian Yangtze Platform. Palaeogeogr. Palaeoclimatol. Palaeoecol. 443, 66–79 (2016).

    Article  Google Scholar 

  19. 19.

    Pi, D.-H., Liu, C.-Q., Shields-Zhou, G. A. & Jiang, S.-Y. Trace and rare earth element geochemistry of black shale and kerogen in the early Cambrian Niutitang Formation in Guizhou province, South China: Constraints for redox environments and origin of metal enrichments. Precambr. Res. 225, 218–229 (2013).

    ADS  CAS  Article  Google Scholar 

  20. 20.

    Cremonese, L., Shields-Zhou, G. A., Struck, U., Ling, H.-F. & Och, L. M. Nitrogen and organic carbon isotope stratigraphy of the Yangtze Platform during the Ediacaran–Cambrian transition in South China. Palaeogeogr. Palaeoclimatol. Palaeoecol. 398, 165–186 (2014).

    Article  Google Scholar 

  21. 21.

    Wang, D. et al. Marine redox variations and nitrogen cycle of the early Cambrian southern margin of the Yangtze Platform, South China: Evidence from nitrogen and organic carbon isotopes. Precambr. Res. 267, 209–226 (2015).

    ADS  CAS  Article  Google Scholar 

  22. 22.

    Godfrey, L. V. & Falkowski, P. G. The cycling and redox state of nitrogen in the Archaean ocean. Nat. Geosci. 2, 725–729 (2009).

    ADS  CAS  Article  Google Scholar 

  23. 23.

    Tesdal, J. E., Galbraith, E. D. & Kienast, M. Nitrogen isotopes in bulk marine sediment: linking seafloor observations with subseafloor records. Biogeosciences 10, 101–118 (2013).

    ADS  Article  Google Scholar 

  24. 24.

    Algeo, T. J., Meyers, P. A., Robinson, R. S., Rowe, H. & Jiang, G. Q. Icehouse–greenhouse variations in marine denitrification. Biogeosciences 11, 1273–1295 (2014).

    ADS  Article  Google Scholar 

  25. 25.

    Zerkle, A. L. et al. Onset of the aerobic nitrogen cycle during the Great Oxidation Event. Nature 542, 465–467 (2017).

    ADS  CAS  Article  PubMed  Google Scholar 

  26. 26.

    Ader, M. et al. Ocean redox structure across the Late Neoproterozoic Oxygenation Event: A nitrogen isotope perspective. Earth Planet. Sci. Lett. 396, 1–13 (2014).

    ADS  CAS  Article  Google Scholar 

  27. 27.

    Cremonese, L. et al. Marine biogeochemical cycling during the early Cambrian constrained by a nitrogen and organic carbon isotope study of the Xiaotan section, South China. Precambr. Res. 225, 148–165 (2013).

    ADS  CAS  Article  Google Scholar 

  28. 28.

    Higgins, M. B., Robinson, R. S., Husson, J. M., Carter, S. J. & Pearson, A. Dominant eukaryotic export production during ocean anoxic events reflects the importance of recycled NH4+. Natl. Acad. Sci. USA 109, 2269–2274 (2012).

    ADS  CAS  Article  Google Scholar 

  29. 29.

    Junium, C. K. & Arthur, M. A. Nitrogen cycling during the Cretaceous, Cenomanian-Turonian Oceanic Anoxic Event II. Geochem. Geophys. Geosyst. 8, 133–157 (2007).

    Article  Google Scholar 

  30. 30.

    Algeo, T. J., Morford, J. & Cruse, A. New Applications of Trace Metals as Proxies in Marine Paleoenvironments. Chem. Geol. 306–307, 160–164 (2012).

    Article  Google Scholar 

  31. 31.

    Zheng, Y., Anderson, R. F., Geen, A. V. & Fleisher, M. Q. Remobilization of authigenic uranium in marine sediments by bioturbation. Geochim. Cosmochim. Acta 66, 1759–1772 (2002).

    ADS  CAS  Article  Google Scholar 

  32. 32.

    Helz, G. R., Bura-Nakic, E., Mikac, N. & Ciglenecki, I. New model for molybdenum behavior in euxinic waters. Chem. Geol. 284, 323–332 (2011).

    ADS  CAS  Article  Google Scholar 

  33. 33.

    Algeo, T. J. & Tribovillard, N. Environmental analysis of paleoceanographic systems based on molybdenum–uranium covariation. Chem. Geol. 268, 211–225 (2009).

    ADS  CAS  Article  Google Scholar 

  34. 34.

    Wille, M., Nägler, T. F., Lehmann, B., Schröder, S. & Kramers, J. D. Hydrogen sulphide release to surface waters at the Precambrian/Cambrian boundary. Nature 453, 767–769 (2008).

    ADS  CAS  Article  PubMed  Google Scholar 

  35. 35.

    Wen, H., Fan, H., Zhang, Y., Cloquet, C. & Carignan, J. Reconstruction of early Cambrian ocean chemistry from Mo isotopes. Geochim. Cosmochim. Acta 164 (2015).

  36. 36.

    Lenton, T. M., Boyle, R. A., Poulton, S. W., Shields-Zhou, G. A. & Butterfield, N. J. Co-evolution of eukaryotes and ocean oxygenation in the Neoproterozoic era. Nat. Geosci. 7, 257–265 (2014).

    ADS  CAS  Article  Google Scholar 

  37. 37.

    Sahoo, S. K. et al. Oceanic oxygenation events in the anoxic Ediacaran ocean. Geobiology 14, 457–468 (2016).

    CAS  Article  PubMed  Google Scholar 

  38. 38.

    Jin, C. et al. A highly redox-heterogeneous ocean in South China during the early Cambrian (529–514 Ma): Implications for biota-environment co-evolution. Earth Planet. Sci. Lett. 441, 38–51 (2016).

    ADS  CAS  Article  Google Scholar 

  39. 39.

    Taylor, S. R. & Mclennan, S. M. The Continental Crust: Its Composition and Evolution, An Examination of the Geochemical Record Preserved in Sedimentary Rocks. (Blackwell Scientific Pub., 1985).

  40. 40.

    Mclennan, S. M. Relationships between the trace element composition of sedimentary rocks and upper continental crust. Geochem. Geophys. Geosyst. 2(4), 203–236 (2001).

    Article  Google Scholar 

  41. 41.

    Och, L. M. et al. Palaeoceanographic controls on spatial redox distribution over the Yangtze Platform during the Ediacaran-Cambrian transition. Sedimentology 63, 378–410 (2015).

    Article  Google Scholar 

  42. 42.

    Na, L. & Kiessling, W. Diversity partitioning during the Cambrian radiation. Proc. Natl. Acad. Sci. USA 112, 4702–4706 (2015).

    ADS  CAS  Article  PubMed  PubMed Central  Google Scholar 

  43. 43.

    Li, G. et al. Early Cambrian metazoan fossil record of South China: Generic diversity and radiation patterns. Palaeogeogr. Palaeoclimatol. Palaeoecol. 254, 229–249 (2007).

    ADS  Article  Google Scholar 

Download references


This study was financially supported by the Ministry of Land and Resources of the People’s Republic of China (MLRPRC) (2009GYXQ15), Chinese Academy of Sciences (CAS) (XDB10010100, XDPB05). Research by Y.F. Li is supported by the China Postdoctoral Science Foundation (2017M610958). Laboratory facilities were provided by CNNC Beijing Research Institute of Uranium Geology. Many thanks to Yu Zhang and Duan Wei for their help in field work, and Guishan Jin for his help in laboratory experiments. Special thanks is given to Thomas J. Algeo from University of Cincinnati, Maoyan Zhu also from NIGPAS and Hongfei Ling from Nanjing University for their constructive suggestions. Finally, we deeply appreciate the insightful comments of Editor and peer reviewers to improve this paper.

Author information




J.Z. designed this research with inputs form T.F., Y.Z., G.L., Y.L. and Y.W., J.Z. and Y.W. collected samples and performed lab analyses. J.Z., Y.Z. and G.L. wrote this paper with significant improvements from all coauthors. J.Z. and Y.W. took and provided all the photos involved.

Corresponding author

Correspondence to Junpeng Zhang.

Ethics declarations

Competing Interests

The authors declare that they have no competing interests.

Additional information

Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Zhang, J., Fan, T., Zhang, Y. et al. Heterogenous oceanic redox conditions through the Ediacaran-Cambrian boundary limited the metazoan zonation. Sci Rep 7, 8550 (2017).

Download citation

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.


Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing