Effects of ATOX-1 knockdown HMEC-1 cells on ATOX-1, VEGF-R2, and CTR-1 protein level and its angiogenic activity. (a) Representative western blot for ATOX-1 (7 kDa cropped band), VEGF-R2 (230 kDa cropped band), and CTR-1 (35 kDa cropped band) protein on whole-cell extracts from HMEC-1 cells. GAPDH (37 kDa cropped band) expression was used as a protein loading control. (b) Representative photographs of HMEC-1 cells in Matrigel™ assays. Photographs were taken after knockdown of ATOX-1 using 60 nM ATOX-1 siRNA C. Non-silencing siRNA (NSsiRNA) was use as control. Inset, % of inhibition as compared to untreated control cells; N.S. non-significant; Scale bar 200 µM. (c) Total surface area of vascular structure. Columns, n = 3, bars, SEM (***p < 0.0001).