Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels

Differentiation of stem cells to chondrocytes in vitro usually results in a heterogeneous phenotype. This is evident in the often detected over expression of type X collagen which, in hyaline cartilage structure is not characteristic of the mid-zone but of the deep-zone ossifying tissue. Methods to better match cartilage developed in vitro to characteristic in vivo features are therefore highly desirable in regenerative medicine. This study compares phenotype characteristics between pericytes, obtained from human adipose tissue, differentiated using diphenylalanine/serine (F2/S) peptide hydrogels with the more widely used chemical induced method for chondrogenesis. Significantly higher levels of type II collagen were noted when pericytes undergo chondrogenesis in the hydrogel in the absence of induction media. There is also a balanced expression of collagen relative to aggrecan production, a feature which was biased toward collagen production when cells were cultured with induction media. Lastly, metabolic profiles of each system show considerable overlap between both differentiation methods but subtle differences which potentially give rise to their resultant phenotype can be ascertained. The study highlights how material and chemical alterations in the cellular microenvironment have wide ranging effects on resultant tissue type.

Induction of mesenchymal stem cells (MSCs) to undergo chondrogenesis requires the cells to have strong cell-cell interactions and that they maintain a spherical morphology. The added use of growth factors in culture media such as transforming growth factors (TGFs) and bone morphogenetic proteins (BMPs) have also been shown to induce chondrogenesis 1, 2 . However, a common observation when inducing MSCs to form chondrocytes in vitro is the expression of type X collagen by the cells [3][4][5][6] . Typically, type X collagen is not expressed in the mid-zone of hyaline cartilage and is characteristic of chondrocytes undergoing hypertrophy and endochondral ossification in the deep-zone region of the tissue 7,8 suggesting that in vitro, the cells differentiate along a mixed phenotypic lineage. Over exposure to compounds such as TGF-β1 is thought to be responsible for increased type X expression 3,6 and, as such, biomaterial systems which are able to influence phenotypic expression are highly desirable as a replacement for chemically induced differentiation.
The use of stem cells for engineering cartilage is of particular interest as they facilitate continual development from chondroblasts to terminal differentiation (hypertrophic chondrocytes), a characteristic which is not observed with the use of chondrocytes 9 . Also, induced differentiation of stem cells is able to provide an abundant source of chondrocytes to compensate for the naturally low cell numbers found in cartilage tissue. Cartilage is an avascular tissue type and naturally occuring cell populations are low. To collect and culture mature chondrocytes up to required population numbers is time consuming and costly. For this reason, prefered approaches for engineering cartilage have been to differentiate stem cells in vitro which can then be used in vivo. Pericytes, or perivascular stem cells, are sourced from the vasculature, inclusive of adipose tissue [10][11][12] . Thus, they are able to meet the much needed demand of a highly abundant multipotent cell type and are well placed for use in cartilage tissue engineering.
Tuning of material mechanical properties (stiffness) is an effective means of targeting a range of MSC fates, inclusive of chondrogenesis [13][14][15] . Supramolecular gels have shown enormous potential as model biomaterials to meet this particular challenge [16][17][18][19] . There have been major successes in the application of self-assembled peptide based materials as instructive matrices for stem cell growth, where the emphasis has been on the inclusion of biochemical signals, usually comprising matrix protein specific peptidic motifs 18 . There has also been focus on chemical approaches to control gel stiffness, either via redesign of building blocks or chemical crosslinking 20 . A number of breakthroughs have shown that stem cells' growth and differentiation, in addition to biochemical signals, are highly sensitive to physical stimuli presented by their immediate environment 21,22 . Specifically, mechanical 21 (i.e. gel stiffness) and structural/topographical factors 23 of the cell-contacting matrix play crucial roles that have, in some cases, been shown to be more powerful than soluble biochemical signals 24 .
Previously, we had shown that pericytes cultured in supramolecular peptide hydrogels were able to undergo differentiation into a number of cell lineages when the hydrogels were tuned to various stiffnesses 25 . In this study, an interesting find was the differentiation of pericytes along the chondrogenic lineage when cells were cultured in 13 kPa hydrogel, contrary to the previously observed myogenic development in other biomaterials with similar stiffnesses 24,26 . The distinction of which can be explained by the use of a nanofiber structured hydrogel which the cells interact differently with compared to crosslinked materials 25 .
As this was an unusual observation for cellular differentiation in mechanically tuned substrates, where cell behaviour is contradictory to the norm, this study aimed to ascertain the properties of chondrocytes that develop in the Fmoc-F 2 /S hydrogels. We do this by further investigating the chondrogenic induction of pericytes, if Fmoc-F 2 /S hydrogels are able to sustain development in the longer term and whether the effective cellular development can be enhanced with the aid of chondrogenic induction media.

Fmoc-F 2 /S hydrogels act as biomaterial substrate to promote chondrogenesis of pericytes.
We recently reported on the use of co-assembled hydrogels of the well-known gelator fluorenylmethoxycarbonyl (Fmoc)-diphenylalanine (F 2 ) 27, 28 and surfactant-like Fmoc-serine (Fmoc-S) to produce cyto-compatible core/shell nanofibers that may be crosslinked upon exposure to cell culture media, resulting in gelation ( Fig. 1A and B) 29 . The mechanical properties of the Fmoc-F 2 /S hydrogels were tuned by careful control of the peptide concentration in the pre-gel liquid before initiating cross linking with introduction to culture media, allowing gelation to occur as published previously 25 . The supramolecular hydrogels were therefore created using peptide concentrations that allow the formation of gels with moduli similar to that reported for chondrons 30,31 .
Oscillatory rheology of the hydrogel shows that the gels possess elastic moduli of 15.5 kPa (Fig. 1D). The G′, elastic modulus, exceeds the viscous modulus G″, signifying that the hydrogel is an elastic material. The nanoscale features and hydrophilic chemistry presented by Fmoc-S on the surface allows nanoscale hydrogel fibres to adsorb proteins which enable indirect contact with cell surface receptors facilitating the cell-material interaction needed to interpret biomaterial qualities (Fig. 1C).
Promotion of chondrogenic development in vitro generally requires that cells are cultured within a three dimensional construct in order to maintain a typical rounded morphology and eliminating the dedifferentiation effects of monolayer culture 32-34 . The formation of aggregates in culture is of particular advantage as it has been shown to promote chondrogenic development in stem cells 35,36 . Pericytes cultured within the 15.5 kPa Fmoc-F 2 /S hydrogels were observed to have good viability with cells forming small clusters (aggregates) over time ( Fig. 2A). Pericyte differentiation was assessed by monitoring gene expression levels of RUNX-2, SOX-9 and type II collagen after 1 week which showed increased expression levels of all three genes compared to the negative control with SOX-9 and type II collagen in particular showing a statistically significant increase (Fig. 2B).
Cells were then cultured over a longer term (5 weeks) within the Fmoc-F 2 /S substrate and were subsequently immunofluorescently stained for type II collagen and aggrecan production. Confocal microscopy imaging demonstrated the presence of both proteins indicating successful differentiation of the pericytes into chondrocytes ( Fig. 2C and D).
Fmoc-F 2 /S hydrogel promotes lessens formation of type X collagen while balancing aggrecan and type II collagen ratios. Phenotypic characteristics of differentiated pericytes were monitored by focusing on the relative expression levels of type II collagen, aggrecan and type X collagen after long-term culture (35 days). Comparisons were carried out using pericytes cultured in the 15.5 kPa Fmoc-F 2 /S gels in the presence (+) and absence (−) of chondrogenic induction medium. In addition, pericytes were also cultured in alginate hydrogels with chondrogenic induction medium in order to make a comparison with a conventionally used hydrogel system for chondrogenesis that is also used in MACI [37][38][39][40][41] . It is noteworthy to mention that many studies which use alginate for chondrogenesis of MSCs, culture the cells using low serum (1% FBS) concentrations. As chondrogenesis was originally observed in Fmoc-F 2 /S substrates using standard basal media composition (10% FBS), this study retains the conditions in which the original observation was made for both alginate and Fmoc-F 2 /S. Confocal microscopy images of immunofluorescently stained F 2 /S hydrogels cultured with pericytes for 28 days. Pericytes were checked for chondrogenic development by staining for aggrecan production (C) and type II collagen (D) both ascertained through green fluorescence. Cell populations are indicated by staining the cell nucleus with DAPI (blue). The images are mosaics of a 3 × 3 tile scan, each acquired from random positions of the hydrogel. Scale bar in A is 100 µm, in C & D is 50 µm. In B, Error bars denote the standard error where p < 0.05 as calculated using unpaired student t-test.
Gene expression profiles of pericytes showed an increase in all cartilage biomarkers over 1 week after which trends held steady up to 35 days indicating differentiation of pericytes. Marker expression levels from pericytes in both Fmoc-F 2 /S+ and Fmoc-F 2 /S− hydrogels were distinctly higher than those in alginate. The Fmoc-F 2 /S+ showed the highest production of SOX-9, type II collagen and type X collagen respectively (Fig. 3). Assessment of collagen and glycosaminoglycan production relative to each other showed that the outcome of this was dependent on which culture system was used. Type II collagen production was highest in Fmoc-F 2 /S+, having on average 2.6 fold higher concentrations compared to aggrecan (Fig. 4A). Aggrecan content was largest when alginate was used (Fig. 4A). Pericytes cultured in Fmoc-F 2 /S− hydrogels exhibited an overall balance between type II collagen and aggrecan expression (0.89x, Fig. 4A).
Over expression of type X collagen compared to type II is known to be prevalent in most in vitro systems that differentiate stem cells into chondrocytes. This phenomenon is thought to be due to the culture system having an over exposure to transforming growth factor β1 (TGF-β1) 3,6 and also because of the use of ascorbic acid [42][43][44] , both of which are typically used in formulating chondrogenic induction medium. While Fmoc-F 2 /S+ and alginate produced the collagen II/collagen X ratio with greater collagen X weighting; it was seen, however, that F 2 /S−, which is absent of the tailored medium, produced the desired ratio with greater collagen II weighting (Fig. 4B), highlighting the influence of the supplemented medium on resultant cellular differentiation.
Chondrocyte metabolome highlights pathways that are causal of phenotypic differences.
Metabolomics data were generated using high-resolution LC-MS analysis of denatured cell extracts of pericytes cultured in the Fmoc-F 2 /S+/− hydrogel systems. Comparisons between these two systems allowed identification of cell processes that are affected by the presence of induction medium and which subsequently lead to differences in phenotypic expression. A generalised overview of the metabolome using hierarchical cluster analysis showed that, on the whole, the metabolite profiles of Fmoc-F 2 /S+/− systems, although distinct from the undifferentiated control set, were similar to each other with slightly higher metabolite abundances detected on the Fmoc-F 2 /S+ substrate (Fig. 5A). Detected metabolite masses were mapped to known pathways to ascertain which areas of Expression levels of all four biomarker were observed as elevated for all culture systems with the highest expression levels noted for pericytes cultured in Fmoc-F 2 /S hydrogels. Gene expression was compared against pericytes cultured on glass cover slips (undifferentiated on planar substrate) as a negative control. Error bars are standard error of the mean; * indicate significant difference between groups as determined by one-way ANOVA followed by Bonferroni post hoc test where p < 0.05; n = 4. metabolism were most differentiated from the control set. The pathways showing the most significant change were those involved in amino acid metabolism and energy generating processes such as the TCA cycle (Fig. 5B). Of these, the most changed significantly were metabolites involved in arginine and proline metabolism. A pathway which contributed to the development of collagen and the synthesis of polyamines. Polyamines are known to play an important role in proliferation and differentiation 45,46 , and in particular, development of chondrocytes 47 .
Closer inspection between Fmoc-F 2 /S+ and Fmoc-F 2 /S− reveal pockets of distinction between the two. These potentially highlight metabolic processes that ultimately result in the phenotypic differences observed in Fig. 4. Sample sets were compared against each other and a nominal threshold of 2 was set to isolate metabolites considered as distinct from one another. From the total population (734), 23.4% of these were considered to be of interest. When mapped to metabolic processes, the pathways that had the greatest number of hits and therefore most contrasting turnover were centred mainly in amino acid metabolism. Observed differences between Fmoc-F 2 / S− and Fmoc-F 2 /S+ therefore indicate differences in the protein make up between both systems. Pathways such as tyrosine metabolism and arginine & proline metabolism, however, showed no significant change between both systems suggesting that they are requisite for chondrocyte development but do not necessarily play a role in the observed shifts in phenotype between Fmoc-F 2 /S− and Fmoc−F 2 /S+ (Fig. 5C).
Significantly changed pathways involved in energy generation, such as the TCA cycle, although different from undifferentiated cells showed no discernible difference between Fmoc-F 2 /S-and Fmoc-F 2 /S+ as cells in both substrates are considered to be similarly active.

Discussion
The use of the biomaterial alone not only instigates pericytes to undergo chondrogenesis but the cells are also able to sustain continual development as observed through the constant production of the chondrogenic markers over a longer time in culture. The use of induction medium with the hydrogels causes a phenotypical imbalance, most notably with the production of collagen. No difference between the two systems (with and without induction media) are noted with regards to aggrecan production, suggesting that induction medium is better tailored to collagen development.
The type of collagen formed, however, is affected by the induction media as generally higher type X collagen content is observed in these systems over type II collagen. This correlates with the gene expression profiles observed in Fig. 2 where initial chondrogenesis of pericytes is assessed. In the absence of the induction media, RUNX-2 expression levels is lowered compared to SOX-9. Subsequently, formation of type X collagen, which is pre-empted by RUNX-2, is lowered and we observe higher type II collagen content. Relative levels of the gene markers in alginate were significantly lower than the Fmoc-F 2 /S hydrogels which may be due to the difference in the cell type used, as opposed to MSCs for example, as well as the deviation from the typical use of low serum concentrations. Notwithstanding, comparative production of chondrogenic markers in alginate show that the use of induction media for differentiation also gives a higher proportion of type X collagen formation over type II collagen. Levels that are comparable with Fmoc-F 2 /S+ (Fig. 4B). Typically observed in vitro, type X collagen imbalance suggests greater population of hypertrophic chondrocytes 5, 6, 48 , thought to occur due to the initiation of some osteogenic activity 3,6 . The detection of type X collagen in the Fmoc-F 2 /S-system however, indicates that its production is not completely eliminated but significantly reduced when chemical induction is avoided.
The interplay between collagen and glycosaminoglycan content is noteworthy as the distribution of one relative to the other in native cartilage plays an important role in the functional output of the tissue as a whole. The zonal structure of native hyaline cartilage, shows increased abundance in glycosaminoglycan content moving (B) Expression of type II collagen relative to type X collagen. Gene expression was compared against pericytes cultured on glass cover slips (undifferentiated on planar substrate) as a negative control. Error bars are standard error of the mean; * indicate significant difference between groups as determined by one-way ANOVA followed by Bonferroni post hoc test where p < 0.05; n = 4.
from the articular surface through the superficial, mid and deep zones where there is the greatest resistance to compressive loading 8,49,50 . Therefore, in order to impart better functionality when healing damaged cartilage tissue, the implication of balancing the abundance of type II collagen and aggrecan when developing cartilage tissue in vitro through mechanical and chemical substrate design is of particular importance, as it should best Metabolites were mapped to metabolic pathways to ascertain which cell processes are significantly changed from the control. (C) Comparisons between Fmoc-F 2 /S− and Fmoc-F 2 /S+ isolate metabolite masses that differ between the two by more than two fold (inset) giving insight into processes that lead to the altered chondrocyte phenotype between Fmoc-F 2 /S− and Fmoc-F 2 /S+ Pathways marked with Ɨ were observed to be significantly different from undifferentiated cells but not between Fmoc-F 2 /S− and Fmoc-F 2 /S+ suggesting influence during chondrogenesis but do not contribute to the observed differences in chondrocyte phenotype.
match the required structure of the lost tissue. The balance achieved with using Fmoc-F 2 /S− (Fig. 4A) allows for potential formation of a tissue type that is not overly inflexible due to higher collagen content than is observed when including induction medium.
While the findings from the metabolomics study are preliminary, they demonstrate correlation of cell behaviour with their microenvironment and a means of acutely discriminating between potential sub-phenotypes of a particular cell type. That is, distinguishing between finer details that cannot be simply reflected in 'positive' detection of specific markers. This however requires further experimental design and implementation beyond the scope of this study.

Rheology.
To assess the mechanical properties of the hydrogels, dynamic frequency sweep experiments were carried out on a strain-controlled rheometer (Kinexus rotational rheometer from Malvern) using a parallel-plate geometry (20 mm) with a 0.50 mm gap. An integrated temperature controller was used to maintain the temperature of the sample stage at 25 °C. Precautions were taken to minimize solvent evaporation and to keep the sample hydrated: a solvent trap was used and the atmosphere within was kept saturated. To ensure the measurements were made in the linear viscoelastic regime, an amplitude sweep was performed and the results showed no variation in elastic modulus (G′) and viscous modulus (G″) up to a strain of 1%. The dynamic modulus of the hydrogel was measured as a frequency function, where the frequency sweeps were carried out between 1 and 100 Hz. The measurements were repeated at least three times to ensure reproducibility.

Transmission electron microscopy (TEM).
Carbon-coated copper grids (No. 400) were glow discharged for 5 s and placed shiny side down on the surface of the hydrogel for less than 5 s. Excess sample was removed by blotting with a filter paper and then 10 mL of negative stain (Nanovan: 1% aqueous methylamine vanadate, obtained from Nanoprobes) was placed on the top of the sample on the grid and allowed to dry for 10 mins. The dried specimens were then imaged using a LEO 912 energy filtering transmission electron microscope operating at 120 kV fitted with a 14 bit/2 K Proscan CCD camera. Fiber diameters were measured using ImageJ software version v1.43 u.
Pericyte isolation. Adipose tissue was collected with prior informed and written consent from healthy adult volunteers (n = 3) undergoing cosmetic lipectomy procedures. Permission for tissue collection and subsequent experimental protocols were granted and carried out in accordance with stated guidelines by the South East Scotland Research Ethics Committees (SESREC 10/S1103/45) in Edinburgh.
Pericytes were isolated from adipose tissue from adult donors undergoing cosmetic liposuction by Flow Activated Cell Sorting (FACS) using a FACS Aria II (BD Biosciences) based on our established protocols 10 and is summarised below.
Cell Culture and Reagents. Pericytes were tripsinised from the culture well flasks, pelleted by centrifugation and resuspended in either the Fmoc-F 2 /S or the alginate solution.
Cell laden alginate beads were made by pipetting 300 µL of the alginate solution into a 100 mM calcium chloride solution. They were allowed to cure at room temperature for 5 minutes before removing the calcium chloride solution. Hydrogels were then washed twice with PBS solution and 500 μl of culture media added to each well. 300 µL of the Fmoc-F 2 /S cells suspension was dispensed into 24 well culture plates containing 500 μl of culture media. Culture plates were incubated under humidified atmosphere of 5% CO 2 at 37 °C for approximately 1 hr to allow the Fmoc-F 2 /S hydrogels to fully cure. Following this, the media in both the peptide and alginate hydrogels were changed every 24 hours in the first two days of preparation and every twice weekly after that.
Cell viability. Culture media surrounding the biomaterials were aspirated to waste and washed once with warm PBS solution. A working solution containing both Syto 10 and ethidium homodimer-2 dyes was made in PBS (1:500 v/v). 500 µL of the dye solution was added to the hydrogels and the samples incubated in the dark at room temperature for 15 minutes.
Following this, the hydrogels were then fixed at room temperature with 4% formaldehyde solution for at least 15 minutes before viewing under a microscope.
Immunofluorescence cell staining. After 28 days in culture (unless otherwise stated), the cells were washed once in PBS and fixed with 10% formaldehyde at 37 °C for 15 min. When fixed, the samples were permeabilised using a buffer solution (10.3 g sucrose, 0.292 g NaCl, 0.06 g MgCl 2 , 0.476 g Hepes buffer, 0.5 ml Triton X, in 100 ml water, pH 7.2) at 4 °C for 5 min. The samples were then incubated at 37 °C for 5 min in 1% BSA/PBS, followed by the addition of the primary antibody (1:50 in 1% BSA/PBS, monoclonal anti-human collagen type II and aggrecan raised in mouse (IgG1), Santa Cruz Biotechnology Inc) for 1 h (37 °C). The samples were then washed in 0.5% Tween 20/PBS (5 min, ×3). A secondary, biotin-conjugated antibody (1:50 in 1% BSA/PBS, monoclonal anti-mouse (IgG), Vector Laboratories, Peterborough, UK) was added for 1 h (37 °C) followed by washing. A FITC conjugated streptavidin third layer was added (1:50 in 1% BSA/ PBS, Vector Laboratories, Peterborough, UK) at 4 °C for 30 min, and given a final wash.
QRT-PCR analysis. RNA extractions from cells cultured on hydrogel biomaterials were done using the Trizol extraction reagent (Invitrogen). Cells cultured for on culture well plastic had RNA retrieved using RNeasy micro kit (Qiagen), both protocols were carried out as per manufacturer's instructions. Reverse transcription to obtain cDNA was done using Quantitech reverse transcription kit (Qiagen) for all samples, also according to the manufacturer's protocol. Cells were cultured and harvested at time points 1hr, 1, 3, 7, 14, 28 and 35 days in culture.
Amplification by qRT-PCR was done using human specific primers (Eurofins MWG Operon) detailed in Table 1. PCR was carried out using a 7500 Real time PCR system & software (Applied Biosystems). Samples had a total reaction volume of 20 µL containing 2 µL of diluted cDNA, each reverse and forward primer at a final concentration of 100 µM and analysed using SYBR green chemistry (Qiagen). For PCR amplification samples were held at 50 °C for 2 minutes then 95 °C for 10 min then amplified using 95 °C for 15 s and 60 °C for 1 min for 40 cycles. The specificity of the PCR amplification was checked with a heat dissociation curve (measured between 60-95 °C) done subsequent to the final PCR cycle. Gene expression levels were standardised using GAPDH as an internal control. Quantification analysis was performed using the comparative ΔΔCt method 51 and gene expression expressed as fold change relative to the control sample.
Samples were assayed in quadruplicate and gene expression was expressed as mean ± SEM.

Metabolomic analysis.
Metabolite extraction from cells cultured on hydrogels and control samples for 1 week was done using ice cold chloroform:methanol:water (1:3:1,v/v) on a shaker for 1 h maintained at 4 °C. Samples were centrifuged and 10 μL of the supernatant injected on to the LC-MS system. The LC separation was carried out using hydrophilic interaction chromatography with a ZIC-HILIC 150 mm × 4.6 mm, 5 μm column (Merck Sequant), operated by an UltiMate liquid chromatography system (Dionex, Camberley, Surrey). The LC mobile phase was run with 0.1% formic acid in water (A) and 0.08% formic acid in acetonitrile (B). The mobile phase was run at a linear gradient for 30 minutes from 20-80% A, maintained at 5% A for 10 minutes and then re-equilibrated to 20% A. Mass spectrometric detection was performed using an Orbitrap Exactive (Thermo Fisher Scientific, Hemel Hempstead, U.K.) within the mass range m/z 70-1400 in polarity switching mode.
Chromatographic peak selection and metabolite identification were done using Ideom/MzMatch excel interface 52, 53 and measured peak intensities by LC-MS were normalised against protein content as measured using the Bradford assay as detailed previously 54 . Metabolite identification was done using a set of known standards to define mass and chromatographic retention times. Putative metabolites were also identified on this basis using predicted retention times as described by Creek et al. 55 .
Statistical Analysis. Analysis of variance (ANOVA) and Bonferroni post hoc tests were performed using GraphPad prism software to compare more than two study groups. Statistical significance is noted where the calculated p value is less than 0.05 using four biological replicates unless otherwise stated.
Multivariate analysis of the LC-MS data and metabolite pathway mapping were done using Metaboanalyst 2.0 56 . Data availability. Raw data generated from this study is available from http://dx.doi.org/10.5525/gla. researchdata.344. article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.