Tumor-Derived Exosomes Induce the Formation of Neutrophil Extracellular Traps: Implications For The Establishment of Cancer-Associated Thrombosis

Cancer patients are at an increased risk of developing thromboembolic complications. Several mechanisms have been proposed to explain cancer-associated thrombosis including the release of tumor-derived extracellular vesicles and the activation of host vascular cells. It was proposed that neutrophil extracellular traps (NETs) contribute to the prothrombotic phenotype in cancer. In this study, we evaluated the possible cooperation between tumor-derived exosomes and NETs in cancer-associated thrombosis. Female BALB/c mice were orthotopically injected with 4T1 breast cancer cells. The tumor-bearing animals exhibited increased levels of plasma DNA and myeloperoxidase in addition to significantly increased numbers of circulating neutrophils. Mice were subjected to either Rose Bengal/laser-induced venous thrombosis or ferric chloride-induced arterial thrombosis models. The tumor-bearing mice exhibited accelerated thrombus formation in both models compared to tumor-free animals. Treatment with recombinant human DNase 1 reversed the prothrombotic phenotype of tumor-bearing mice in both models. Remarkably, 4T1-derived exosomes induced NET formation in neutrophils from mice treated with granulocyte colony-stimulating factor (G-CSF). In addition, tumor-derived exosomes interacted with NETs under static conditions. Accordingly, the intravenous administration of 4T1-derived exosomes into G-CSF-treated mice significantly accelerated venous thrombosis in vivo. Taken together, our observations suggest that tumor-derived exosomes and neutrophils may act cooperatively in the establishment of cancer-associated thrombosis.

microvesicles are relatively large size (0.1-1 μm diameter) membrane fragments shed from the cell surface and exosomes, which are composed of particles preformed within multivesicular bodies that fuse with the plasma membrane to release vesicles between 50 and 200 nm 5,6 . Several studies have correlated the increased plasma levels of tumor-derived tissue factor (TF)-positive microvesicles with a prothrombotic state in both animal models [7][8][9] and patients 10 . However, this correlation has not been observed in a number of cancer types including gastric, colorectal, brain and breast cancer [11][12][13] . From these data, two explanations have been proposed: 1) TF-independent mechanisms may operate to favor cancer-associated thrombosis; 2) EVs in the nanoscale range (exosomes), which have not been evaluated in most of the thrombosis studies, may have an underestimated role.
Increased leukocyte count is a well-known predictor of cancer-associated thrombosis 14,15 . In fact, recent studies have shown that neutrophils play an important role in arterial 16 and venous 17 thrombus development. The involvement of neutrophils in thrombus formation is dependent on the formation of neutrophil extracellular traps (NETs) 18,19 , which are described as web-like structures of DNA and proteins formed through a process called NETosis 20,21 . The negatively charged surface of NETs results in the activation of contact phase proteins such as FXII. In addition, NETs activate platelets and inactivate local endogenous anticoagulants 22,23 . Therefore, treatment with DNase I, which degrades NETs, severely impairs the development of deep vein thrombosis in mice 18,19 .
NETs have been proposed as important players in cancer-associated thrombosis 15,[22][23][24] . Demers and colleagues showed that neutrophilic mouse models of cancer exhibit systemic markers of NET formation and neutrophils that are more prone to NET formation ex vivo 23,25 . Tumor-associated neutrophilia and propensity to NET formation seems to be directed correlated with increased levels of granulocyte colony-stimulating factor (G-CSF) [23][24][25] . These tumor models also exhibit spontaneous thrombus formation. Remarkably, the prothrombotic state could be reproduced by treating tumor-free mice with G-CSF combined with low doses of LPS 23 .
In the present study, we employed a murine breast cancer model to address the possible cooperation between tumor-derived exosomes and neutrophils in the establishment of cancer-associated thrombosis. Our results demonstrate that NET formation is crucial for the acceleration of venous and arterial thrombus formation in tumor-bearing mice. Remarkably, tumor cell-derived exosomes induce NET formation in neutrophils from G-CSF-treated mice and accelerate venous thrombus formation in tumor-free neutrophilic mice. Our observations suggest that tumor-derived exosomes and neutrophils act cooperatively in the establishment of cancer-associated thrombosis.

Results
Mice bearing 4T1 tumors exhibit a NET-dependent prothrombotic state. The 4T1 murine mammary carcinoma cell line is a highy aggressive and metastatic cell line whose orthotopic inoculation into female BALB/c mice induces the rapid growth of solid tumor masses 26 . Moreover, 4T1 tumors have been described as potent G-CSF producers 23,27 , resulting in the dramatic elevation of circulating neutrophils. Here, we observed increased platelet, monocyte, lymphocyte and neutrophil counts in mice bearing 4T1 tumors, compared to control mice, 3 weeks after inoculation ( Fig. 1A-E). The tumor-bearing mice displayed systemic signs of neutrophil activation and NET induction, as indicated by increased plasma levels of extracellular DNA (Fig. 1F) and myeloperoxidase (Fig. 1G).
The systemic signs of neutrophil activation in 4T1-bearing mice were previously associated with microthrombosis, which coincides with the appearance of NETs 23 . Here, we evaluated the involvement of NETs in tumor-associated venous or arterial thrombosis. To address this question, we first employed a venous thrombosis model in which thrombus formation was induced by Rose Bengal/laser photochemical injury in the jugular vein. Figure 2A shows that the occlusion times were significantly lower in tumor-bearing mice than in controls (27.9 ± 2.2 min vs. 56.7 ± 3.2 min). Next, mice were subjected to a model of arterial thrombosis in which thrombus formation was triggered in the carotid artery by injury with ferric chloride. As seen in the venous model, 4T1-bearing mice exhibited a significant reduction in occlusion time in comparison to control animals (9.8 ± 0.9 min vs. 32.7 ± 6.6 min) (Fig. 2B). To determine the participation of NETs in the prothrombotic state, mice were treated with DNase 1 prior to thrombosis induction. Remarkably, in the venous thrombosis model, the occlusion times of control and tumor-bearing mice were very similar upon treatment with DNase 1 (49.7 ± 5.4 min vs. 49.6 ± 3.3 min) ( Fig. 2A). Interestingly, treatment with DNase 1 abolished arterial thrombus formation in most of the animals regardless of the presence of 4T1 tumors, suggesting that NETs are essential for thrombus formation upon ferric chloride-induced vessel injury. Arterial thrombi were further analyzed by immunofluorescence. As expected, thrombi from 4T1-bearing mice were highly enriched in cells that stained for Ly6G, a well-recognized marker for peripheral neutrophils 28 , as compared to thrombi from control mice (Fig. 2C). In addition, a strong co-localization of Ly6G with extracellular fiber-like DNA was observed, indicating the presence of NETs (Fig. 2D).

Tumor-derived exosomes induce NET release. Several studies have demonstrated that tumor-derived
EVs (microvesicles and exosomes) modulate host immune cells and thus contribute to cancer progression 5,29 . Quantitative analysis of EVs in plasma revealed a significantly higher number of particles in tumor-bearing mice than in controls (Fig. 3A). Nanotracking analysis showed that most of the particles are in the 80-110 nm range, suggesting the accumulation of exosome-like vesicles (Fig. 3B). These data were confirmed by the increased levels of CD63, a known exosome marker 30 , in the plasma of 4T1-bearing mice in comparison to controls (Fig. 3C).
To evaluate whether tumor-derived exosomes could influence NET release, we harvested exosomes from cultured 4T1 cells and evaluated their effect on isolated mouse bone marrow cells. Most of the 4T1-derived exosomes were within the same size range (100-120 nm) of the particles observed in the plasma of tumor bearing-mice (Fig. 4A). The increased predisposition of tumor-bearing mice for NET formation has been attributed at least in part to the increased G-CSF levels in these mice. In this context, we treated bone marrow cells from G-CSF-treated mice with 4T1-derived-exosomes. We noticed a significant increase in the amount of extracellular filamentous DNA derived from Ly6G + cells in the cells treated with exosomes ( Fig. 4C) compared to the untreated bone marrow cells (Fig. 4B). The extracellular fibers were positive for citrullinated histone staining, thus confirming that exosomes derived from tumor cells induce NET formation by G-CSF-primed neutrophils (Fig. 4D).
NETs serves as scaffold for tumor-derived procoagulant exosomes. The procoagulant properties of tumor cells have been mainly attributed to expression of the clotting initiator protein, tissue factor (TF) 31,32 . In this context, flow-cytometric analyses revealed high levels of TF antigen on the surface of 4T1 cells (Fig. 5A). Several studies demonstrate that the presence of TF in EVs is strongly dictated by the TF expression status of their cells of origin 33,34 . Thus, we further evaluated the procoagulant properties of 4T1-derived exosomes. Figure 5B shows that exosomes exhibit a dose-dependent procoagulant effect. The recruitment of procoagulant EVs into the growing thrombus has been proposed as important event during thrombus developmet 35,36 . At this point,  we observed that 4T1 cell-derived exosomes can adhere to NETs in vitro under static conditions (Fig. 5C). These results suggest that NETs may recruit tumor-derived exosomes thus contributing to the prothrombotic state in 4T1-bearing mice.

Treatment with G-CSF and exosomes recapitulates the prothrombotic state of tumor-bearing mice.
To evaluate the effect of G-CSF-induced neutrophilia and tumor-derived exosomes in the mouse prothrombotic state, we employed the venous thrombosis model in tumor-free mice. Treatment with G-CSF efficiently increased the number of peripheral neutrophils, as shown in Fig. 6A. Neutrophilia induced by G-CSF was sufficient for reducing the occlusion times compared to naïve mice (37.1 ± 2.3 min vs. 54.0 ± 2.6 min), as shown in Fig. 6B. Less significant changes were observed upon intravenous administration of 4T1-derived exosomes in G-CSF-untreated mice (43.5 ± 0.3 min). Remarkably, the occlusion times observed in G-CSF-treated mice, which were further infused with 4T1-derived exosomes (26.6 ± 1.6 min), were similar to those recorded for tumor-bearing mice (27.9 ± 2.2 min). Further immunohistochemical analysis confirmed the increased presence of Ly6G + cells and DNA fibers in thrombi from G-CSF/exosomes-treated mice (Fig. 6C).

Discussion
Several studies have demonstrated that neutrophils actively participate in both arterial and venous thrombus formation. There is strong evidence that most of the prothrombotic properties of neutrophils derive from the formation of neutrophil extracellular traps (NETs). In vitro and in vivo studies have shown that NETs display several pro-hemostatic effects including the stimulation of platelet aggregation, activation of contact pathway, and degradation of natural coagulation inhibitors 37,38 . Remarkably, Demers and colleagues 23,25 demonstrated that murine cancer models that rely on significant neutrophilia predispose NET formation through mechanisms that have not been fully elucidated.
Here, we have employed a murine breast cancer model that develops a dramatic increase in leukocyte count, a process that parallels the systemic signs of NET formation. In this study, tumor-bearing mice exhibited accelerated arterial thrombus formation in the ferric chloride model. Importantly, we have employed a mild ferric chloride treatment, which has been described as critical for discriminating specific prothrombotic stimulus such as increased FVIII levels 39 . Treatment with DNase 1 abolished thrombus formation in most of the tumor-bearing mice. In fact, we could not observe thrombus formation in most of the tumor-free mice that were treated with DNase 1 prior to challenge with ferric chloride. Other studies have demonstrated that neutrophils are essential for thrombus formation in a mouse model of laser-induced injury 16,40 . Moreover, blocking NET formation in lupus and atherosclerosis mouse models delays arterial thrombus formation induced by photochemical injury in the carotid artery 41,42 . In addition, impaired DNase 1-mediated degradation of NETs was recently associated with the manifestation of acute thrombotic microangiopathies in humans 43 . It remains to be determined whether a similar mechanism operates to enhance cancer-associated thrombosis.
Tumor-bearing mice also exhibited a significant acceleration of venous thrombosis in the Rose Bengal/ laser-induced model. Interestingly, tumor-bearing animals treated with DNase 1 exhibited occlusion times that were identical to tumor-free mice. Most remarkably, treatment with DNase 1 produced no effect on tumor-free animals. This is strikingly distinct from a recently described mouse model of deep vein thrombosis based on partial flow restriction, in which treatment with DNase 1 or the depletion of neutrophils prevented thrombus formation in most of the animals 17,19 . Therefore, the photochemical injury model may be helpful for evaluating the contribution of the innate immune system to cancer-associated thrombosis.
The 4T1 murine breast cancer model is characterized by a dramatic increase in the number of circulating neutrophils, with G-CSF being a major determinant for this acquired phenotype 23,25,27 . Treatment with G-CSF in humans has long been associated with neutrophil activation 44 and, more recently, with elevation of NET  Exosomes were labeled with DilC18 (red), and NET DNA was stained with Hoechst 33342 (blue). Cells were isolated and stimulated with PMA for 3 hs to induce NET formation, before incubation with 4T1 exosomes. Scale bar = 20 μm.
formation markers 45,46 . Herein, we observed a significant acceleration of venous thrombus formation in mice treated with G-CSF, although the extent of acceleration was not as dramatic as observed in tumor-bearing animals. G-CSF involvement in an intricate pro-metastatic mechanism involving neutrophils and gamma delta T cells was recently identified 47 . In addition, Kowanetz and colleagues demonstrated that G-CSF mobilizes neutrophils to premetastatic sites, thus increasing the metastatic potential of a number of murine cell lines, including 4T1 cells 27 . Alternatively, blocking G-CSF significantly reduces the number of spontaneous metastases in this cell model.
The increasing number of circulating extracellular vesicles (EVs) present during cancer progression has been widely documented in humans and animal models 2, 10, 48 . EVs have been linked with several aspects of tumor biology, including their ability to interact and modulate host immune cells 48,49 . In this study, we observed that 4T1-derived EVs, in the exosome size-range, induce NET formation in neutrophils derived from G-CSF-treated mice. Remarkably, the infusion of tumor-derived exosomes into G-CSF-treated animals accelerated thrombus formation to a degree similar to that observed in tumor-bearing animals. Thomas and colleagues recently demonstrated the ability of NETs to trap tumor-derived microvesicles in a murine pancreatic cancer model 50 . In fact, the infusion of high-TF but not low-TF tumor-derived microvesicles enhanced deep vein thrombosis. In our study, we observed that NETs interact with 4T1-derived exosomes. Therefore, NETs may function as scaffold for tumor-derived EVs, either exosomes or microvesicles, thus enhancing their effects on thrombosis in addition to other biological effects. Tumor-derived exosomes have been involved in pre-metastatic niche formation 49   Taken together, our results demonstrate that NET formation is crucial for the acceleration of venous and arterial thrombus formation in tumor-bearing mice. 4T1-derived exosomes induce NET formation in neutrophils derived from G-CSF-treated mice and accelerate venous thrombus formation in tumor-free neutrophilic mice. Therefore, we propose that tumor-derived exosomes cooperate with neutrophils in the establishment of cancer-associated thrombosis (Fig. 7). This process might be of particular relevance in tumors that rely on increased G-CSF production and the subsequent neutrophilia that predisposes NET formation. Remarkably, Thålin and colleagues showed that increased G-CSF levels correlates with NET-associated microthrombosis in cancer patients 46 . A parallel increase in tumor-derived EVs may facilitate NET formation both in the tumor microenvironment and systemically, which in turn may impact the occurrence of thrombotic manifestation, primary tumor growth 52 and metastasis 53 and vascular damage 54 . Therefore, strategies aimed at preventing NET formation, such as elastase and PAD4 inhibitors [55][56][57] , may have a significant impact on tumor progression.  carbonate, Hepes Buffer and 10% FCS (fetal calf serum) and maintained at 37 °C in a humidified atmosphere of 5% CO 2 . The cells were kept in culture no more than five passages before inoculation into mice.
Animals. BALB/c mice were housed under controlled temperature (24 ± 1 °C) and light (12 h light starting at 7:00 a.m.) conditions. All in vivo experiments were conducted in accordance with the approved guidelines of the institutional care and use committee from the Federal University of Rio de Janeiro. The animal procedures were approved by the Institutional Committee under protocol number IBQM/089-07/16.

Tumor induction.
Female mice (8-10 wk-old) were inoculated with 5 × 10 4 4T1 cells in the fourth mammary fat pad. The animals were monitored every other day for tumor growth, and the tumors were measured with a caliper. Tumor volume was calculated using the formula V = (l × w 2 ) × 0.4, where l is the length and w the width.
Blood cell analysis. The mice were anesthetized as described, and blood was collected into EDTA (1.8 mg/ mL) by cardiac puncture. A blood cell count was automatically determined using the CELL-DYN 3500 hematology analyzer (Abbott Diagnostics, Abbott Diagnostics, Illinois, USA).
Quantification of plasma DNA. Plasma DNA levels were measured using the Quant-it Picogreen dsDNA kit according to the manufacturer's instructions. Briefly, 5 µL of plasma from control or 4T1 tumor bearing mice and 50 µL of Picogreen (diluted in TRIS buffer) were added per well in an opaque 96-well plate. Fluorescence was measured in an automated spectrofluorimetric reader (Spectra Max Paradigm; Molecular Devices, Menlo Park, CA, USA) at 485 nm excitation and 535 nm emission.

Quantification of myeloperoxidase and CD63 in plasma.
Myeloperoxidase was quantified with a colorimetric assay. Briefly, 50 µL of plasma from control or 4T1 tumor bearing mice was incubated with 25 μL 3,3′,5,5′-tetramethylbenzidine and 50 μL H 2 O 2 . The mixture was incubated for 5 minutes at 37 °C and the reaction was interrupted by the addition of 100 μL H 2 SO 4 (2 M). The absorbance was determined at 405 nm using a Spectra Max Paradigm spectrophotometer. The level of CD63 in the plasma of control or 4T1 tumor-bearing mice was determined using a mouse CD63 specific ELISA kit according to the manufacturer's instructions. The results obtained in our experiments were calculated by standard curve interpolation and expressed in pg/mL. Venous thrombosis model. Mice were anesthetized as described above. The jugular vein was isolated through a midline cervical incision, and blood flow was continuously monitored using a 0.5 PSB Doppler flow probe coupled to the TS420 flow meter. Rose Bengal dye (75 mg/kg body weight) was diluted to 15 mg/mL in PBS and administered via the lateral tail vein. Just before injection, the jugular vein was transilluminated with a 1. Arterial thrombosis model. The evaluation of arterial thrombosis was performed as previously described 58 with minor modifications. In brief, control or 4T1-tumor bearing BALB/c mice were anesthetized with intramuscular xylazine (16 mg/kg) followed by ketamine (100 mg/kg). The right common carotid artery was isolated through a midline cervical incision, and blood flow was continuously monitored using a 0.5-VB Doppler flow probe coupled to a TS420 flow meter (Transonic Systems, Ithaca, NY, USA). Thrombus formation was induced by applying a piece of filter paper (1 × 2 mm) saturated with 5% FeCl 3 solution on the adventitial surface of the artery for 2 min. After exposure, the filter paper was removed and the vessel was washed with sterile saline. Carotid blood flow was continuously monitored for 60 min or until complete occlusion (no flow for at least 3 min) occurred. For the experiments with DNase 1, the mice were treated intravenously with 10 µg of Pulmozyme ® , 15 min prior to the induction of thrombosis.
Isolation of exosomes from plasma samples and cell culture supernatants. The plasma obtained from control or 4T1 tumor-bearing mice was centrifuged for 1000 g for 10 min. The resulting supernatant was further diluted in PBS and ultra-centrifuged (Beckman Coulter Optima LE-8OK, 70 Ti rotor) at 100,000 g for 1 h at 4 °C. The samples were washed once with PBS and centrifuged again. The pellet was resuspended in ice cold PBS, filtered with a 0.2 μm filter and kept at −20 °C until analysis. Exosomes were isolated from the cell culture medium using ExoQuick-TC ™ according to the manufacturer's instructions. The supernatant obtained from 4T1 cells cultured for 48 h in serum free media, was centrifuged at 3000 g for 15 min to remove debris and then incubated overnight at 4 °C with ExoQuick-TC ™ . This mixture was sequentially centrifuged for 15 and 5 min at 1500 g. The supernatant was removed and the pellet was resuspended in ice cold filtered PBS and stored at −20 °C for no more than one week. Exosome protein content was measured using the Pierce Modified Lowry Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer's instructions.
SCIenTIFIC REPORTS | 7: 6438 | DOI:10.1038/s41598-017-06893-7 Nanoparticle tracking analysis. Nanoparticle tracking analysis was performed using a NanoSight LM10 microscope (NanoSight Ltd, Salisbury, UK). The samples were diluted in PBS. Five videos of 30 seconds were taken for each sample with the detection threshold set at 15. The size and concentration of the samples were determined after analysis of the recorded videos with NTA software (version 2.3). The blur, minimum track length and minimum expected particle size parameters used for the analyses were set automatically.
In vitro NET formation. Bone marrow cells were obtained from untreated mice or mice subcutaneously treated with 2.5 μg of G-CSF for 3 days. The cells were then harvested by flushing the femoral bone. The cell suspension was washed with PBS and resuspended in high glucose DMEM (GIBCO). The cells (1 × 10 5 ) were then seeded onto 13 mm cover-slips (Glasscyto) and stimulated with 0.1 µg of 4T1-derived exosomes. After incubation for 3 h at 37 °C, cells were fixed with 500 µL of 4% PFA for 10 min, washed 3 times with PBS and incubated for 10 min with blocking solution (PBS, 10% FBS, 5 mg/ml BSA). The samples were then incubated with rabbit polyclonal anti-histone H3 antibody (diluted 1:50 in blocking solution (ab5103, Abcam) and anti-LYG6 antibody conjugated with AlexaFluor 488 (1:100 dilution, 53-5931-80, eBioscience). After three washes with blocking solution, the slides were incubated with goat anti-rabbit IgG labeled with rhodamine (1:500 dilution) (31670, ThermoFisher Scientific) and Hoechst 33342 at 1:1000 for 2 h. Images were captured with a confocal microscope (LEICA DMI 4000 with laser system TCS SPE) and analyzed using Fiji Image J software. NETs were identified on the digitalized images as Hoechst-positive fibrils emanating from cells, with an overall length at least twice as long as the cell diameter, and were counted in at least five fields of view per treatment group. The results were expressed as the number of NETs/50 Ly6G + cells counted.
Flow-cytometric analyses. For surface detection of TF, 5 × 10 5 4T1 cells were resuspended in PBS containing 1% BSA and incubated for 30 min at 4 °C with a rabbit polyclonal antibody against murine TF (200 μg/ml; #4515, American Diagnostica, Stamford, CT, USA). After washing to remove unbound antibody, cells were incubated with a phycoerythrin-conjugated anti-rabbit IgG (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA). Cells were then washed again and acquired using a BD FACScaliburTM Flow Cytometer (Becton Dickinson). Data were analysed by the BD CellQuest ProTM software (Becton Dickinson).
In vitro activation of plasma coagulation. Procoagulant activity of 4T1-derived exosomes was measured by a clotting assay employing normal pooled mouse platelet-poor plasma (PPP). Fifty microliters of exosomes resuspended in PBS at different concentrations were added to 50 μL of PPP containing 3.8% sodium citrate diluted 1:9 (v/v). After 1 min incubation at 37 °C, 100 μL of 25 mM CaCl 2 was added and the clotting times were recorded on a KC4 Delta Coagulometer (Tcoag Ireland Limited, Wicklow, Ireland).

Interaction of exosomes with NETs under static conditions. Bone marrow cells were obtained
from BALB/C mice as described above. The cells (1 × 10 5 ) were then seeded onto 13 mm cover-slips (Glasscyto) and stimulated with 50 nM PMA, a potent inducer of NETosis 20 . After incubation for 3 h at 37 °C, cells were incubated with 1 μg of 4T1-exosomes. Exosomes used in these experiments were previously stained with 3 mM DiIC18 (1,1′-dioctadecyl-3,3,3,3′-tetramethyl-indocarbocyanine perchlorate), a fluorescent lipophilic compound (Invitrogen) that intercalates cell membranes. Cells were further fixed with 500 µL of 4% PFA for 10 min, washed 3 times with PBS and incubated for 10 min with blocking solution (PBS, 10% FBS, 5 mg/ml BSA). The samples were then incubated with Hoechst 33342 at 1:1000 for 2 h. Images were captured with a confocal microscope (LEICA DMI 4000 with laser system TCS SPE) and analyzed using Fiji Image J software. Statistical analysis. The results were expressed as the mean ± SEM or were depicted as scatter plots.
Statistical analyses were performed using GraphPad Prism 5 (GraphPad Software). One-way analysis of variance (ANOVA) followed by Tukey's post test was used for comparisons between the test groups. If only two groups were compared, an unpaired 2-tailed Student's t-test was applied. Differences were considered significant when P < 0.05.