Figure 6 | Scientific Reports

Figure 6

From: High-fidelity de novo synthesis of pathways using microchip-synthesized oligonucleotides and general molecular biology equipment

Figure 6

De novo synthesis of lycopene biosynthetic pathway. Fragments were assembled by multiplex assembly using error-depleted oligo pools of various throughputs and half-specific primer pairs. Thirty six (a), 3–4 (b), 6–7 (c), and 17–19 (d) subpools were used in one assembly reaction and 24, 26, 24, and 22 fragments were assembled, respectively. (e) Fragment detection in the assembly (36 subpool/reaction) with full-specific primer pairs. (f) Fragment detection in the assembly (12 subpool/reaction) with half-specific primer pairs. (g) Fragment detection in the assembly (12 subpool/reaction) with full-specific primer pairs. (h) Operons assembled by Gibson assembly. M1, 20 bp DNA ladder (TaKaRa Bio); M2, 2 K plus II DNA ladder (TaKaRa Bio). Red arrows indicate target fragments. OLS, oligo subpool; F, fragment.

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