Inhibition of nitric oxide synthesis prevents DOX-mediated cardiotoxic effects in hCSs and iCSs. (a) Statistical analysis (n = 6) of DOX-mediated cardiotoxic effects in hCSs and iCSs evaluated as a ratio between dead and live cells after 24 h treatment. 100 μM L-NIO prevents the doxorubicin-mediated effects in both hCSs and iCSs treated with 10, 20 and 40 μM doxorubicin. (b) Statistical analysis (n = 6) of DAF-FM-positive cells in hCSs and iCSs following 24 h doxorubicin treatment. *Relative to control for all groups; ♯Within the same concentration. Data are presented as mean ± SEM. One-way ANOVA followed by Bonferroni’s multiple comparisons test. F (df 10, 55)=40.53 for (a) hCS, F (df 10, 143) = 253 for (a) iCS, F (5, 30) = 37.33 (b) hCS and F (5, 30)=56.09 for (b) iCS. See also Fig. S4.