Table 1 Functional evaluation matrix for BeWo, ACH-3P, JAR and Jeg-3 cell models.

From: A comparative study of five physiological key parameters between four different human trophoblast-derived cell lines

  Cell line
BeWo ACH-3P JAR Jeg-3 Primary model systems
Tight junctions (ZO-1) + + + + +64
NaFlu retardation + + ~ + +64
TEER (day 5) 169.33 ± 3.05 340.44 ± 34.31 179 ± 4.31 308.66 ± 27.95 323.91 ± 35.4564
Glucose transport (60 min) 56.9 ± 7.6% 55.2 ± 4.1% 52.0 ± 4.3% 9.1 ± 0.1% <40%62 (term placental explants, 120 min) <5%64 (primary trophoblast cells, 60 min)
hCG release (apical/basal) [ng/ml] 1.26/1.45 2.87/5.09 1.6/1.9 1.11/1.22 130.5664/−
hCG release (50 µm forskolin stimulus) [fold increase] 5.14 2.58 2.58 4.73 N/A
Polystyrene nanoparticle cut-off (F-to-M ratio of 50 nm/490 nm particle permeability) 0.19 ± 0.001/0.102 ± 0.02 0.37 ± 0.05/0.04 ± 0.02 0.19 ± 0.01/0.21 ± 0.05 0.33 ± 0.05/0.24 ± 0.01 0.4/0.05 (placental perfusion model)28