Proteomics analysis of exosomes isolated from MM and colostrum. (a) A four-way Venn diagram of proteins distributed between 24, 48, 72 h colostrum and MM exosome samples revealing 1372 proteins common to all dataset. (b) Heatmaps showing proteins that are 3-fold more abundant in exosomes isolated from 24 h colostrum samples compared to MM. A distinct pattern was revealed where proteins that were seen to be highly abundant in 24 h colostrum exosomes steadily diminished in 48 and 72 h colostrum exosomes and reached similar levels to the MM exosomes. (b) Proteins that are 3-fold more highly abundant in exosomes isolated from MM samples compared to 24 h colostrum sample were also plotted as a heatmap and revealed a similar distribution pattern.