Stoichiometry of natural bacterial assemblages from lakes located across an elevational gradient

Heterotrophic bacteria are thought to be phosphorus-rich organisms with relatively homeostatic stoichiometry, but the elemental composition of natural bacterial communities has rarely been assessed. Here we tested whether bacterial stoichiometry changes with the trophic status of lakes by assessing the elemental composition of the bacterial-dominated (hereafter microbial) fraction together with that of the dissolved and seston fractions in 11 lakes situated along an elevational gradient. The stoichiometry of these three size-fractions was analyzed during the thermal stratification and mixing periods in composite water samples and in the water layer of the deep chlorophyll-a maximum. In addition, we analyzed the relative abundance of the most common bacterial groups in the lakes. Our results show that the microbial fraction was always enriched in phosphorus compared to the dissolved fraction, irrespectively of the lake trophic status. Further, they indicate that the elemental composition of bacteria in mountain lakes is at least seasonally very dynamic, resulting not only from changes in the nutrient ratios of the resource itself, but probably from changes in the composition of the dominant bacterial taxa too, though at the taxonomic level analyzed, we did not find evidence for this.

Scientific RepoRts | 7: 5875 | DOI: 10.1038/s41598-017-06282-0 faster growing organisms have an increasing demand on P to be allocated to the P-rich ribosomal RNA 21,22 . Thus, fast growing organisms are more P-rich than slow growing ones. Indeed, different freshwater bacterial groups grow at very different rates 23 and it is plausible that that this theory also applies to bacteria. For example, Betaproteobacteria and particularly the R-BT subgroup (see also Limnohabitants) grow faster than other bacterial groups 23 .
Here we assessed the stoichiometry of the microbial (0.7-1 µm) fraction in lakes located along an elevational gradient (567-2799 m above sea level) during the summer stratification period, when all lakes exhibited a well-developed deep chlorophyll-a maximum, and in autumn, when most lakes have already undergone mixis. For comparison, we also analyzed the stoichiometry of the dissolved (<0.7 µm) and seston (0.7-50 µm) fractions. We collected composite samples from the whole water column (CWS) and additionally, from the deep chlorophyll-a maximum (DCM). Though the presence of phototrophic bacteria in the microbial fraction cannot be completely excluded, autotrophic picoplankton in mountain lakes are either present in very low abundances or are absent 24 . Thus, we hypothesized that the elemental composition of the microbial fraction dominated by heterotrophic bacteria will differ among lakes of different trophic status (e.g., based on DOM and total phosphorus concentrations) as found along an elevational gradient 25,26 . In other words, we expected that the microbial fraction will be more depleted in nutrients (N and P) relative to carbon in the more oligotrophic lakes situated higher in the elevational gradient. Additionally, we assessed the bacterial community composition to test whether in lakes dominated by the fast growing R-BT subgroup of Betaproteobacteria, the stoichiometry of the microbial fraction is more P-rich than in lakes where other taxonomic groups dominate the community.

Results
Stoichiometry of the dissolved fraction. Nutrient concentrations were always lower in water samples collected in lakes above the treeline than in those from lakes below it (Supplementary Table S1 and Table S2). TDP concentrations in July ranged from 0.30 to 5.30 µg L −1 and from 0.80 to 4.40 µg L −1 in October. The highest DOC concentration (5157 µg L −1 ) was measured in the CWS from Lansersee (LAS) and the lowest one (237 µg L −1 ) in Oberer Plenderlesee (OPL). TDN concentrations showed a large variability ranging from 50.02 µg L −1 in Schwarzsee ob Sölden (SOS) to 4329.07 µg L −1 in LAS (Supplementary Table S2).
In the dissolved fraction, the C:P ratio was always higher than the Redfield ratio (Table 1) and this pattern was more pronounced in lakes situated at low elevation. The C:N ratios were generally lower than the Redfield ratio (6.6). Three of the subalpine lakes had higher C:N ratios than the average (1.5) and two of them had a C:N higher than the Redfield one (Table 1). Stoichiometry of the microbial and seston fractions. The C:P ratios of the microbial fraction were in almost all cases higher than the Redfield ratio (Table 2). In most lakes, the microbial fraction was P-depleted relative to C in July (mean C:P: ~345) with the exception of OPL and Drachensee (DRA). In July, the microbial fraction of these two lakes exhibited a C:P very close to 106, whereas in October the microbial fraction of all lakes was poor in P compared to C. The microbial fraction of the four subalpine lakes situated at low elevation (i.e., 567-1180 m a.s.l.) and that of Gossenköllesee (GKS) and Sebensee (SES) was slightly N-depleted compared to Redfield (6.6). In July, the C:P ratios of the seston fraction showed the same trend as that of the microbial fraction.
The seston of almost all lakes was P-depleted relative to C, especially in the subalpine lakes and in the alpine lakes   GKS and SES. The C:N ratios in the subalpine lakes Baggersee Rossau (BAR), Lansersee (LAS), Piburgersee (PIB), and Wildsee (WIS), as well as in the alpine lakes GKS and SES showed a depletion, all other lakes again had a slightly lower C:N ratio than the Redfield Ratio (6.6). However, this trend was more pronounced in July than in October (Table 2). Water temperature was not significantly correlated with changes in stoichiometric ratios in the two fractions, except for the C:N ratio of the seston fraction in the CWS ( Supplementary Fig. S1). Finally, the C:P ratio of the microbial and seston fractions was not significantly correlated with that of POC:chlorophyll-a ( Supplementary Fig. S2) suggesting that detritus did not affect the trends observed.
Bacterial abundance and community composition. Bacterial abundance in the CWS and DCM samples ranged from 1.92 × 10 5 cells mL −1 to 2.80 × 10 6 cells mL −1 in July and from 1.85 × 10 5 cells mL −1 to 2.62 × 10 6 cells mL −1 in October ( Supplementary Fig. S3). In July, lake bacterial abundance decreased steadily along the elevational gradient, but in October this trend was not so obvious. The bacterial assemblage was dominated by Actinobacteria in seven out of eleven lakes in October ( Supplementary Fig. S4). By contrast, in July Betaproteobacteria was the dominant group in five out of ten lakes or co-dominated the bacterial assemblage with Actinobacteria in two other lakes. In July, relative abundance of Bacteroidetes was between 10-25% of DAPI-stained cell in lakes situated at intermediate and high elevation (WIS, BREN, OBB, SES, DRA, OPL, GKS), whereas in October their relative abundance decreased notably in the alpine lakes SES, DRA, OPL, and GKS ( Supplementary Fig. S4). Generally, Alphaproteobacteria accounted for less than 9% of DAPI-stained cells in all lakes with the exception of SOS in October, where their relative abundance reached 21% of DAPI counts. Probe GAMMA 42a targeting Gammaproteobacteria was only used in the lowest lakes to increase the coverage of bacterial community with the group-specific probes. However, Gammaproteobacteria represented <1% of DAPI counts in those lakes and only in LAS in October, their relative abundance reached 3% of DAPI-stained cells ( Supplementary Fig. S4). In July, a significant positive relationship between the relative abundance of R-BT Betaproteobacteria and of Bacteroidetes and lake elevation was found ( Supplementary Fig. S5).

Comparison of regressions.
The slopes and intercepts of the regressions between C and P, N and P, as well as C and N for the two types of water samples (CWS and DCM) did not significantly differ (one-way ANCOVA) in July except for the slope of the N versus C in the seston fraction. Thus, the data from both types of samples were pooled together and a new linear regression was calculated for each fraction and sampling period ( Fig. 1 and Fig. 2, Supplementary Table S3). A significant difference in the slope and intercept of the C versus N relationship between the seston and microbial fraction was found in July (Supplementary Table S3). In October, the slope of the N versus P and of C versus N relationship differed significantly between the pooled microbial and pooled seston fraction (Supplementary Table S3). The relationship between C and P, N and P, and C and N were described by significantly different regression lines in July as compared to October for both the seston and the microbial fraction ( Fig. 1, Fig. 2 and Supplementary Table S3), but not for the dissolved fraction (Supplementary Table S3).

Discussion
The main objective of this study was to assess whether the stoichiometry of the microbial fraction, usually dominated by bacteria 10 , differs among lakes situated along an elevational (trophic) gradient reflecting changes in the stoichiometry of the dissolved fraction (resource). Several factors such as water temperature and cell growth rate influence bacterial stoichiometry, however, resource elemental composition is supposed to be the most important one 10,27,28 . We found that dissolved nutrient concentrations differed greatly among lakes (Supplementary Table S2). As expected, subalpine lakes had higher absolute dissolved nutrient and DOC concentrations. However, in those lakes the nutrient ratios of the dissolved fraction, particularly the C:P ratios, were as high or even higher than in most of the alpine lakes (Table 1), for example, in Baggersee Rossau (567 m a.s.l) was 2124:1 in July versus 832:1 in Gossenköllesee (2413 m a.s.l.). The reason for this unexpected result is mainly the disproportionally high DOC concentrations in the subalpine lakes as compared to their P concentrations. DOC concentrations in the subalpine lakes situated at lower elevations were on average, one order of magnitude higher than in the alpine lakes, whereas TDP concentrations were 3-7 times higher. In the littoral zone of some of the subalpine lakes, emergent macrophytes were prevalent. These primary producers might contribute to the production of autochthonous DOM that are usually enriched in carbon as compared to other nutrients 29 . Our results are also in agreement with those of Kopáček et al. 25 , where higher dissolved C:P ratios were found in lakes with forested catchments (DOC:TN:TP ratio of 983:75:1) than in those situated above the treeline (DOC:TN:TP ratio of 302:77:1). Although the C:P ratios we found were higher than those in the study of Kopáček et al. 25 , they support the trend observed by these authors.
The main processes contributing to external nutrient inputs to lakes are the export from the terrestrial catchment and atmospheric deposition. Nutrients transported through the atmosphere can originate both far or near the lakes. For oligotrophic alpine lakes, long distance sources such as soil-derived dust from desert regions, combustion sources, agriculture, and industry are very important 30,31 . By contrast, local P sources such as pollen, plant fragments, primary biogenic aerosols such as for example, bacterial detritus, can be relevant for lakes situated in forested areas 32,33 . A possible explanation for the different dissolved nutrient ratios found between lakes situated above and below treeline are changes in the relative importance of the mentioned external nutrient sources and a differential retention of nutrients in the soil of the catchment surrounding the lakes 33 . The leaching of phosphorus retained in soils is often associated with DOC leaching, which is low for alpine catchments due to poor soil development and to low microbial activities 33 . Thus, terrestrial export in subalpine lakes is characterized by higher C:P ratios than in alpine lakes. Further, the relative importance of terrestrial nutrient sources decreases across the elevational gradient, whereas that of atmospheric deposition, characterized by lower C:P ratios, tend to increase with elevation 33,34 . Thus, atmospheric nitrogen and phosphorus pollution could further alter the stoichiometry of alpine lakes in the future 35 .
While the C:P ratios of all lakes in our study were well above the Redfield ratio ( Fig. 1. and Fig. 2), the C:N ratios in the dissolved fraction (except for the lakes Wildsee bei Seefeld and Piburgersee) were below it and showed less variability than the corresponding C:P ratios. This indicates that most of the lakes considered were enriched in N and only some of the subalpine lakes showed a slight relative nitrogen depletion.
Although the dissolved fraction was more P-depleted relative to C in the subalpine lakes, the microbial fraction was only more P-depleted in lakes below the treeline during the summer stratification, whereas in autumn no trend was related to the elevation of the lakes. This is probably due to more production and competition with autotrophs in summer. Nevertheless, it seems that the microbial fraction stoichiometry does not directly reflect the changes in the stoichiometry of the dissolved fraction occurring along the elevational gradient. This pattern could be masked by a temperature effect because average values were higher for subalpine than for alpine lakes and this could in fact affect bacterial growth and relative growth rates. However, we did not find a significant relationship between water temperature and the elemental ratios of the microbial fraction ( Supplementary Fig. 1). This is probably because natural bacterial communities are locally adapted to the in situ water temperatures 36 .
The fact that C:P and N:P ratios of the microbial fraction were always lower than those of the dissolved fraction has important consequences for the function bacteria have in these lakes. Bacteria may constrain primary production in P-limited ecosystems because of their competitive advantage in taking up inorganic P and act therefore, rather as sink than as link for nutrients 10 . Stoichiometrically flexible bacteria have the capacity of switching from competitors to regenerators through feedback mechanisms that affect phytoplankton production and indirectly the available carbon resources used by bacteria 27,37 . Bacteria can regulate their elemental composition via homeostatic control within a much more narrow range than is possible in their natural environment 22,27 . However, P-limited bacteria are neither P-rich nor invariant in nutrient content, but rather adapt their elemental composition to the element currently limiting (C or P) 28 . In fact, individual bacterial populations can switch from biomass C:P homeostasis in P-depleted surroundings to no homeostasis under nutrient replete conditions 28,37 . In some of the studied lakes, the C:P ratio of the dissolved fraction was 11-fold higher than the C:P ratio of the microbial fraction and a similar trend was observed for N:P ratios. Only in Sebensee in October, the N:P ratio of the dissolved fraction showed a slightly lower value than that of the microbial fraction. This suggests that most lakes in our study present P limitation, no matter at which elevation they are located.
Microbes cope with nutrient limitation following mainly two strategies 38 and this have different effects on stoichiometric strategies, i.e., homeostoichs vs. heterostoichs 37 . One strategy (competition specialists) is based on the "growth rate hypothesis" (GRH) 9 , and focuses on the increase of growth rates and consequently of the assembly machinery. Fast growing cells increase the production of nucleic acid and ribosomes containing N and P, which are needed to achieve high protein synthesis levels. Another possible strategy (i.e., uptake specialist) is to focus on resource acquisition machinery which corresponds to nutrient uptake proteins which contain N and C, but less P 39 . In natural bacterial communities, the observed stoichiometry probably results from the sum of the multiple strategies exhibited by individual bacterial populations ranging from strict to no homeostasis 28,37 . Further studies are needed to identify which strategy of stoichiometric regulation is used by the dominant bacterial populations in alpine and subalpine lakes.
Finally, another important factor to consider when analyzing the stoichiometry of the microbial fraction is the diversity of this fraction by itself. Different bacterial groups might have different uptake abilities for diverse nutrients and exhibit different degrees of homeostasis 27,28 . At present, we lack an appropriate method that allows assessing concomitantly the taxonomy of bacteria and their elemental composition. Nevertheless, we observed some interesting trends in the composition of the bacterial community across the elevational gradient. Unfortunately, those trends could not be directly related to changes in the microbial fraction stoichiometry. The bacterial assemblage of our study lakes was co-dominated by Actinobacteria and Betaproteobacteria. We found that Actinobacteria generally dominated the bacterial community at both sampling dates, but Betaproteobacteria was the second most abundant group in most lakes. This is in agreement with previous studies in alpine lakes 40,41 and in other freshwater systems [42][43][44] . Betaproteobacteria are considered as a fast growing group 45 . Thus, we expected that due to their fast growth rates and according to the GRH, the microbial fraction of lakes dominated by this group could be P richer than that of systems dominated by other groups. Among Betaproteobacteria, the R-BT subgroup is the one that exhibits the highest growth rates, with doubling times as short as 16 h 23,46 . These doubling times are the highest reported for a freshwater bacterial group. In July, we observed a significant increase of the relative importance of the R-BT subgroup with elevation. However, the C:P ratio of the microbial fraction did not always decrease accordingly and the microbial fraction of the alpine lake Gossenköllesee, where the R-BT bacteria represented a substantial part of the bacterial community (up to 33%) had a C:P ratio as high as that of Piburgersee, where R-BT represented a smaller fraction of the bacterial assemblage.
In summary, given the pivotal role of heterotrophpic bacteria in the nutrient cycles of aquatic ecosystems, our results strengthen the idea of heterotrophic bacteria being a 'sink' for phosphorus, as in all studied lakes they tended to immobilize phosphorus, whereas they might act as nitrogen mineralizers ('link'). Nevertheless, the stoichiometry of the microbial fraction showed a large variability among mountain lakes with C:P and N:P ratios well above the Redfield ratio. Thus, our results further suggest that bacterial assemblages may not be as nutrient-rich as compared to other food-web components, but instead have to be seen as spatio-temporal dynamic in their stoichiometry. While our work could link these changes to the resource itself, understanding how stoichiometric changes in the microbial fraction relate to bacterial community composition will need further studies.

Methods
Sampling sites and main characteristics. To assess changes in the stoichiometry of the different fractions along an elevational gradient, lakes distributed above and below the treeline (range: 567-2799 m a.s.l.) were selected. Lakes situated above the treeline or alpine lakes have usually small catchments areas composed of bare rocks with poorly developed soils and scarce vegetation 47 . Alpine lakes are at the very end of the trophic scale and have very low DOC (<1000 µg L −1 ) and phosphorus concentrations (<4.0 µg L −1 ). The second group of lakes sampled, consisted of subalpine lakes. Those have larger catchment areas surrounded by trees or meadows with well-developed soils and vegetation. In consequence, DOC and nutrient concentrations are higher than in alpine lakes. These lakes show a lower water transparency and higher water temperatures 47,48 . Overall, 7 subalpine lakes and 4 alpine lakes were sampled, although one of the alpine lakes, Schwarzsee ob Sölden (SOS), was only sampled in autumn. The main characteristics of the sampling sites are summarized in Supplementary Table S1.
Sampling. Samples were collected in July 2013 during the summer stratification and in October, when most of the lakes had already undergone the autumn overturn. During each sampling period, the lakes were sampled within two weeks of each other to minimize temporal variability. Water samples were collected from a boat over the deepest point of the lake using a modified 5-L Schindler-Patalas sampler (5 L). Before samples were collected, all containers and bottles were washed with diluted HCl (0.1 N) and rinsed thoroughly with MQ water (Millipore) and then, with water from the respective lake. Temperature, pH, conductivity, dissolved oxygen, and chlorophyll-a (i.e., relative fluorescence) were recorded with a YSI 6600-V 2 multiparameter probe. Two different water samples were collected in every lake, namely, a water sample (10 L) from the deep chlorophyll-a maximum (if it was detectable) and a composite sample (10 L) from the whole water column (i.e., equal volume collected at 1 m depth intervals, n = 5-17 depending on maximum depth). Water from the deep chlorophyll-a maximum (DCM) was collected to determine whether the stoichiometry of the microbial and seston fractions at this depth differed from that of the average for the water column. The depth of the chlorophyll-a maximum was assessed with the YSI 6600 V2 probe equipped with a 'chlorophyll' sensor which detects the fluorescence emitted by chlorophyll-a upon excitation with blue light (470 nm LED).
Size fractionation of water samples. Water collected from the DCM and the composite water sample (CWS) were separated into three different size fractions defined operationally by the type of filter used: the dissolved fraction (<0.7 µm), the microbial fraction (0.7-1 µm) and the seston fraction (0.7-50 µm). All filtrations were done in the laboratory in the shortest possible time (hours) following sampling. A 50 µm nylon mesh net was used to pre-screen the whole water and to obtain the seston fraction. To collect the microbial fraction, the lake water was then pre-filtered through a 1 µm acid-rinsed polycarbonate filter (47 mm, Millipore). Subsequently, each filtrate (seston and microbial fraction) was filtered onto double pre-ignited (3 h at 450 °C) glass-fiber filters (GF/F filters, 25 mm, Whatman with a retention efficiency of 0.7 µm) to collect the biomass for the analysis of their carbon and nitrogen content with a CHN analyzer. The selection of GF/F filters for defining the "dissolved" fraction was done according to Godwin & Cotner 37 . Due to the different particle load of every lake, different volumes were concentrated onto the GF/F filters. The exact volume of the filtrate was recorded for every fraction and used for later calculations. After the filtration steps, the filters were dried at 60 °C for at least 48 h. All filtrations were run in duplicate.
Water chemistry. All water samples were collected in prewashed polyethylene containers and stored at 4 °C till chemical analyses were run (within 24 h). To assess the stoichiometry of the dissolved fraction, we measured dissolved organic carbon (DOC), total dissolved nitrogen (TDN) and total dissolved phosphorus (TDP). DOC and TDN concentrations were measured with a total organic carbon analyzer (Shimadzu TOC-Vc series) equipped with a TNM−1 nitrogen module. For the analyses, 30 mL of the sample were filtered through a pre-treated (4 h at 450 °C) glass-fiber filter (GF/F filters, Whatman) and collected into a pre-treated glass bottle (4 h at 450 °C), acidified to pH 2 with HCl, and stored at 4 °C in the dark until the analyses were made. Both parameters (DOC and TDN) were detected simultaneously after combustion and catalytic oxidation of the Scientific RepoRts | 7: 5875 | DOI:10.1038/s41598-017-06282-0 injected sample. Calibration and comparison with a consensus reference material was done as described elsewhere 49 . Before TDP analyses were made, samples were filtered through pre-treated GF/F filters, as above. The TDP concentration was assessed by spectrophotometry using the molybdate blue method after digestion of the sample with sulfuric acid and hydrogen peroxide 50 .

Stoichiometry of the seston and microbial size fraction. For particulate organic carbon (POC) and
nitrogen (PON) analyses, the dried GF/F filters were analyzed with a Flash EA 1112 (Thermo Fisher Scientific) CHN analyzer. Empty tin capsules and unused pre-treated GF/F filters were used as blanks. For calibration, acetanilide was used at five different concentrations. Total particulate phosphorus (TPP) content of the microbial and seston fractions was calculated by subtracting the total dissolved phosphorus concentration (TDP) in the GF/F filtrate from the total phosphorus concentration (TP) in either the <1 filtrate or the <50 filtrate (Supplementary Table S4). The TP concentration of the seston and microbial filtrates was determined using the molybdate blue method 50 . To test whether detritus could have an effect on the seston and microbial C:P ratios observed, we used the POC to chlorophyll-a ratio as proxy 51 . The chlorophyll-a values used in this test were those obtained from spectrophotometric measurements (acetone extraction) done as described elsewhere 49 . Bacterial abundance in whole and size-fractioned samples. Immediately after sampling, aliquots from the whole and pre-filtrated (<1 µm) water were taken. Samples to estimate bacterial abundance were fixed with 0.2 µm pre-filtered formaldehyde (2% final concentration). Later on, 5 to 10 mL sub-samples were filtered onto black polycarbonate filters (GTTP, 0.2 µm, Millipore) and stained with DAPI (4′,6′-diamidino-2-phen ylindole) 52 . For DAPI counting, an epifluorescence microscope (Zeiss Axioplan) was used. To determine bacterial abundance, 400 to 500 cells were counted in 20-40 randomly chosen microscopic fields. Because the GF/F filters have a retention efficiency defined as 0.7 µm, but no precise pore size, some bacteria might pass through those filters. Therefore, we assessed bacterial abundance in the filtrate obtained after filtration through single and double GF/F filters. Bacterial cell losses due to the filtration through a single GF/F filter represented on average 7.2% of bacterial abundance, but only 5.2% when two filters were used ( Supplementary Fig. S6). We also checked for possible bacterial cell losses in the <1 µm filtrate as compared to the respective whole water sample, however, bacterial abundance was not significantly different (df = 21; p > 0.05).

Bacterial community structure (CARD-FISH). Catalyzed reported deposition-fluorescence in situ
hybridization (CARD-FISH) was used to assess the bacterial community structure 53 . Whole water aliquots were fixed with formaldehyde (2% final concentration), filtered onto white polycarbonate filters (GTTP, 0.2 µm, Millipore) and stored at −20° until analysis. Thawed filters were embedded in 0.1% agarose to prevent cell loses and permeabilized according to Sekar et al. 54 . Six group-specific 5′-horseradish peroxidase (HRP) labeled oligonucleotide probes (ThermoFisher-Hybaid) were used in this study to get an overview of composition of the bacterial community. Probes targeting Bacteria (EUBI-III), Alphaproteobacteria (ALF968), Betaproteobacteria (BET42a), R-BT subgroup of Betaproteobacteria (R-BT065), Ac-I lineage of Actinobacteria (AcI-852), Bacteroidetes (CF319a), and Gammaproteobacteria (GAM42a) were used. To exclude the possibility of a false targeting, we tested this latter probe with a positive control (E. coli culture) that confirmed that the probe worked properly.
Filter sections were placed in 0.5 mL reaction vials containing HRP-labeled probe and the hybridization buffer (formamide concentration 55%.) in a 1:100 ratio. Hybridizations were run for 2:15 hour in a hybridization oven set at 35 °C. Afterwards, the samples were washed first in a preheated washing buffer (10 min at 37 °C) containing the appropriate NaCl concentration and then in 1x PBS buffer (10 min, RT). The signal-amplification step was run for 30 min. For this purpose, filter sections were incubated at 37° with a substrate mix containing the amplification buffer and Alexa Fluor 488 tyramide in a 1:100 ratio. Following the amplification step, filter sections were washed with 1x PBS (15 min, RT and dark), MQ water (10 min, RT) and 96% ETOH. Subsequently the filter sections were dried and mounted on slides using an anti-fading solution containing DAPI (1 µg mL −1 ). All CARD-FISH preparations were examined using a Zeiss Axioplan microscope equipped with a 100 W Hg lamp and filter-sets 1 and 9 for DAPI and Alexa 488, respectively. A minimum of 350 DAPI-stained cells were counted on each filter section. Probe-specific positive cells were recorded from the same microscopic field as DAPI-stained cells. Thus, all CARD-FISH results are expressed as percentage of DAPI-stained cells.
Statistical analyses. One-way ANCOVA was used to compare the slopes and intercepts of the linear regressions obtained between elements (C vs N, C vs P and N vs P) in the microbial, seston and dissolved fraction. The elemental concentrations of P, N, and C were plotted against each other and a linear regression model was fitted to the data. To check for differences in bacterial abundance in filtrates obtained after filtration through GF/F and 1 µm polycarbonate filters to whole water, a paired t-test was used. All statistical analyses were run using the free PAST software.