Figure 1: | Scientific Reports

Figure 1

From: Novel small RNA spike-in oligonucleotides enable absolute normalization of small RNA-Seq data

Figure 1

Small RNA spike-in design and use as a tool to estimate absolute small RNA levels. (a) Design of small RNA spike-in oligonucleotides. Key features of small RNA spike-ins are shown in different colors corresponding to the key. Molar amounts of oligonucleotides added per µg of total RNA are indicated in parentheses. (b) Scatter plot of relative small RNA spike-in levels (reads per million genome-matching reads) compared to absolute small RNA levels (molecules detected per µg of total RNA) in Col-0 flowers (biological replicate 1). Pearson’s r value is indicated, as well as a dashed line that represents a linear model derived from the plotted values. (c) Density plot of individual miRNA family levels in Col-0 flowers (biological replicate 1) in molecules detected per µg of total RNA. Vertical dashed line indicates the median number of molecules per miRNA family.