Effects of the lead compound #G43 and its inactive analog #G43-D. (A) Chemical structures of lead and its inactive analog. Docking poses of (B) Compound #G43 in blue skeleton and (C) compound #G43-D in pink skeleton. Three key residue interactions are depicted by displaying residue chains. (D) Effects of active and inactive compound on the activity of Gtfs by zymographic assays. Glucan zymographic assays (bottom panel) were performed using SDS-PAGE analysis of Gtfs from culture supernatants of S. mutans UA159 incubated with vehicle control DMSO, the synthesized active #G43, and its derivative at 50 µM. SDS-PAGE analysis of Gtfs (top panel) was used as a loading control. (E) Fluorescent microscopy images of S. mutans UA159 biofilms treated with DMSO control, the synthesized #G43, and its derivative #G43-D at 100 µM. Viable bacterial cells were stained with 2.5 µM Syto9 (green).