CEP55 expression was clinically correlated with the activation of nuclear factor kappaB (NF-κB) signalling in patients with pancreatic cancer (PANC). (a) Changes in mRNA expression of NF-κB-regulated genes (MYC, CCND1, BCLXL, MMP9, TWIST, VEGFC and IL6) in nine pancreatic cancer tissues assessed by real-time PCR. (b) Western blotting analysis of CEP55 expression in these nine pancreatic cancer tissues (Upper panel). The endogenous NF-κB activity in these nine pancreatic cancer tissues detected by an electrophoretic mobility shift assay (EMSA) (Lower panel). (c) The Spearman correlation coefficient was calculated to assess the significance of association between CEP55 protein and NF-κB activity. *P < 0.05.