Figure 1 | Scientific Reports

Figure 1

From: Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes

Figure 1

Work flow of study design and sample processing. Plasma from T1DM (n = 36) and control subjects (n = 36) was collected. Exosomes were isolated by ultracentrifugation and characterized by TEM and NTA analysis. Total exosome RNA was isolated and use for the miRNA microarray analysis (discovery set) or for the qRT-PCR validation (validation set) (A). Plasma exosomes were analyzed under electron microscopy which displayed the same morphology in T1DM and control subjects (B,C). Particle and Size distribution of exosomes analyzed by the Nanoparticle tracking analysis of T1DM and control subjects (D,E). Exosomal RNAs determined by the Agilent RNA Pico Chip. Exosomal RNA samples contained no detectable 18S and 28S rRNAs (G). Validation of selected exosome protein expression by flow cytometry (control read peak) (F). Small RNA Densitometry traces profiles were used to quantify and compare the relative abundance of various small RNAs in T1DM (H) and Control subjects (I).

Back to article page