Figure 2 | Scientific Reports

Figure 2

From: Brain regionalization genes are co-opted into shell field patterning in Mollusca

Figure 2

Optical section series of Gbx-expression in the metamorphic competent trochophore larva (65 hpf) of the polyplacophoran Acanthochitona crinita. Dorsal (d)-ventral (v), anterior (a)-posterior (p), and left (l)-right (r) axes indicate the orientation. Anterior faces up in all micrographs. (ac) Sequence of three consecutive optical sections through a metamorphic competent trochophore larva. Gbx is expressed in cells of the shell fields, close to the mouth (asterisk) in the area of the pedal commissure, and in the spicule-bearing cells of the perinotum (arrowhead). Gbx-expressing cells are located in the outermost cell layer of the mantle that constitutes the shell fields. A reflection scan of the boxed region shown in (b) is highlighted in (d). (d) Reflection scan showing Gbx-expression in six rows of cells (arrowheads) in the shell fields (Dapi nuclear counterstain in blue). The seventh row is not included in this micrograph. (e) Fluorescent in situ hybridization (FISH) staining showing Gbx–expression in the spicule-bearing cells (arrowheads) in the area of the prospective perinotum (DAPI nuclear counterstain in blue). Staining in the prototroch (pt) is unspecific. (f) Cells of the pedal (pc) and visceral nerve cords (vc) express Gbx. Scale bars: 20 µm.