Figure 5 | Scientific Reports

Figure 5

From: Midnolin is a novel regulator of parkin expression and is associated with Parkinson’s Disease

Figure 5

MIDN is necessary for parkin expression in PC12 cells. (a) MIDN, parkin, TH, and GAPDH protein levels in MIDN-positive and -negative PC12 cells were examined by Western blotting (left panel). The density of the parkin and TH bands was normalized to that of GAPDH, and these ratios were expressed as a percentage of MIDN-positive cells (middle and right panels). Parkin protein expression was significantly inhibited by MIDN knockout (*p < 0.05, n = 6) whereas TH protein expression was not significantly changed (n = 6). (b) Gene expression levels of MIDN and parkin in MIDN-positive and -negative PC12 cells were examined by qRT-PCR. Significant differences in both MIDN and parkin gene expression were observed between MIDN-positive and -negative cells (*p < 0.05, n = 6).

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