Cystic fibrosis transmembrane conductance regulator (CFTR), known as a cAMP-activated Cl− channel, is widely expressed at the apical membrane of epithelial cells in a wide variety of tissues. Of note, despite the abundant expression of CFTR in mammalian kidney, the role of CFTR in kidney disease development is unclear. Here, we report that CFTR expression is downregulated in the UUO (unilateral ureteral obstruction)-induced kidney fibrosis mouse model and human fibrotic kidneys. Dysfunction or downregulation of CFTR in renal epithelial cells leads to alteration of genes involved in Epithelial-Mesenchymal Transition (EMT) and kidney fibrosis. In addition, dysregulation of CFTR activates canonical Wnt/β-catenin signaling pathways, whereas the β-catenin inhibitor reverses the effects of CFTR downregulation on EMT marker. More interestingly, CFTR interacts with Dishevelled 2 (Dvl2), a key component of Wnt signaling, thereby suppressing the activation of β-catenin. Compared to wild type, deltaF508 mice with UUO treatment exhibit significantly higher β-catenin activity with aggregated kidney fibrogenesis, which is reduced by forced overexpression of CFTR. Taken together, our study reveals a novel mechanism by which CFTR regulates Wnt/β-catenin signaling pertinent to progression of kidney fibrosis and indicates a potential treatment target.
Kidney fibrosis is a common histological manifestation of functional decline in most cases of end-stage kidney diseases. Fibrosis is a reactive process that develops in response to excessive epithelial injury and inflammation, leading to myofibroblast activation and an accumulation of extracellular matrix (ECM)1,2,3. While the cellular origin of myofibroblasts remains to be a subject of intensive debate, numerous animal and clinical studies have pointed to epithelial-to-mesenchymal-transition (EMT) from the tubular epithelial cells as one of the major contributors leading to the generation of myofibroblasts and overproduction of interstitial ECM4,5,6,7,8,9. A multitude of biochemical signaling pathways has been proposed to be associated with the development of kidney fibrosis10,11,12. While Wnt/β-catenin signaling is functionally important in normal kidney development13, 14, aberrant activation of Wnt/β-catenin signaling and subsequent downstream targets, such as matrix metalloproteinases (MMPs), fibronectin and snail, has been observed in the progression of tubulointerstitial kidney fibrosis in unilateral ureteral obstruction (UUO) model15,16,17 and different types of kidney diseases18,19,20,21. Of interest, β-catenin is predominantly up-regulated in renal tubular epithelium of the fibrotic kidneys, suggesting that tubular cells are the major targets of canonical Wnt signaling. In addition, activation of β-catenin in tubular epithelial cells induces EMT, as well as the expression of several fibrosis-related genes in vitro 22,23,24. These results suggest that activation of tubular β-catenin probably plays a critical role in the pathogenesis and progression of kidney fibrosis16. However, it remains unclear how the Wnt/β-catenin signaling pathway is over-activated in fibrotic kidney, and how this signal initiated from the tubular epithelial cells is converted into pathological changes in the tubulo-interstitial space leading to excessive deposition of ECM and fibrogenesis.
Cystic fibrosis transmembrane conductance regulator (CFTR), known as a cAMP-activated Cl− channel, is widely expressed at the apical membrane of epithelial cells in a wide variety of tissues25, 26. Dysfunction of CFTR causes cystic fibrosis (CF), the most common lethal autosomal recessive disease in Caucasians27. Of note, despite the abundant expression of CFTR in mammalian kidney which is localized at the apical surface of proximal tubules and distal tubules28, no overwhelming primary renal disease has been associated with CF. Nevertheless, as CF patients age and have an increased exposure to potentially nephrotoxic agents, such as antibiotics and high glucose, development of chronic renal disease has been commonly recognized29, 30. Given that CF-related nephropathies are frequently compounded by increasing age, pulmonary infection, malnutrition, liver dysfunction and insulin insufficiency31, chronic kidney damage has been considered as secondary manifestation due to the abnormalities in salt transport, infection, and development of cystic fibrosis related diabetes (CFRD)29, 32. One study involving 12 000 patients followed up for a median of 4 years reported an overall annual prevalence of stage 3 chronic kidney disease of 2.3%33. In addition, permanent microalbuminuria occurs in more than 6% of patients with cystic fibrosis30. Despite all these link, the exact role of CFTR in the pathogenesis of chronic kidney diseases has never been investigated.
Interestingly, a recent comprehensive study on human lung epithelial cells has found that the CFTR interactome includes several hundred proteins, with β-catenin listing as a potential interacting protein34. In addition, both our group and other groups have reported that CFTR interacts with β-catenin in intestinal epithelial cells, dysregulation of which leads to aggregated inflammation and development of cancer35, 36. On the other hand, TGF-β and hypoxia inducing factor α (HIFα), key inducers of kidney fibrosis, have been demonstrated to suppress the expression and/or localization of CFTR in the context of epithelial cells37,38,39. Based on these observations, we hypothesized that dysregulation of CFTR might lead to aberrant activation of Wnt/β-catenin pathway and development of kidney fibrosis. We undertook the present study to investigate this possibility using kidney epithelial cell lines, UUO mouse model and CFTR mutant mice in conjunction with genetic manipulation in vitro and in vivo.
Materials and Methods
Cell lines and treatment
Renal distal tubular Madin–Darby canine kidney (MDCK) (ATCC, CRL-2936) cells were cultured in α-MEM medium (GIBCO, NY, USA), supplemented with 10% Fetal Bovine Serum (FBS). Human proximal tubule (HK-2) cells were cultured in DMEM/F12 medium (GIBCO, NY, USA), supplemented with 10% FBS. Cells were maintained in 5% CO2 at 37 °C, and seeded into 6 well plates at 5 × 104/well and incubated in growth medium supplemented with 2% FBS during treatment. CFTRinh-172 (Sigma, MO, USA) was used at the final concentration of 10 µM, whereas GlyH101 (Santa Cruz, California, USA) was used at 2.5 μM, 5 μM and 10 μM for 2 or 3 days as indicated. DMSO (0.1%) at the same volume was used as vehicle control. The effect of hypoxia was achieved by incubating cells in 1% O2–5% CO2 - balance N2 chamber for 1 and 2 days before further assay. Cells incubated under normoxia condition were used as control.
Plasmids and transient transfection
To knockdown CFTR expression in MDCK cells, miRNAs duplex specific to canis familiaris CFTR was synthesized by Life Technologies (Carlsbad, CA). The siRNA sequence is as follows: 5′- ACT TGT TAC TCC TTT CCA A -3′. MiRNA against Lac-z was used as the control sequence. pcDNATM 6.2-GW/EmGFP encoding a pre-miRNA sequence was constructed using BLOCK-iTTM Pol II miR RNAi Expression Vector kit (Life technologies). MDCK cells were seeded on 35 mm culture dishes and 3 μg of vectors were transiently transfected with 6 μl Lipofectamine 2000 (Invitrogen) following the manufacturer’s instruction. The GFP signals were used to evaluate the transfection efficiency. 48 hours after transfection, cell lysates were collected for protein assay to confirm the transfection efficiency.
The ribozymes were generated and used for CFTR knockdown in HK-2 cells as described in our previous study40. For knockdown experiments, 3 μg DNA was transfected into HK-2 cells with 6 μl Lipofectamine 2000 (Invitrogen) and selected by 8 μg/ml Blasticidin S for 2–3 weeks. The stable transfectants were maintained in medium containing 4 μg/ml Blasticidin S.
Total RNA was isolated and reversely transcribed using standard protocols. For quantitative PCR, assays were performed in triplicate on an Applied Biosystems 7500 Fast Real-Time PCR System. The primers were listed in Supplementary Tables 1 and 2.
Protein was extracted using lysis buffer (20 mM Tris HCl pH 8, 137 mM, NaCl10% glycerol, 1% Nonidet P-40 (NP-40), 2 mM EDTA and protease inhibitor). Cell lysates was incubated with primary antibody: anti-CFTR (Millipore MAB3482, clone MM13-4; US) or anti-Dvl2 (Cell signaling technology CST 3224 S, clone 30D2; US). After incubation, the complexes were precipitated using the protein A/G plus-agarose immunoprecipitation reagent, washed and eluted for Western blot assay.
3000 cells/well in 200 μL growth medium were seeded into 96-well plate. Following the appropriate incubation period, medium was removed and 100 μL full medium with 20 μL MTS (3-(4, 5-dimethylthiazol-2-yl)-5-(3carboxymethoxyohenyl)-2-(4-sulfop henyl)-2H-tetrazolium, Promega) reagent was added. The experimental plate was further incubated for 2 hours to allow the bio-reduction of metabolically active cells. The intensity of soluble product was measured by spectrophotometer at 490 nm. Three replicated wells were measured for each experimental group.
Western blot analysis
Cell or tissue protein were extracted using lysis buffer [RIPA: 50 mM Tris, 150 mM NaCl, 1% Triton X-100, 0.1% SDS and 1% nadeoxycholate (pH 7.4)] supplemented with protease inhibitors including phenylmethylsulfonyl fluoride and pimix. The samples were incubated with RIPA at room temperature for 30 minutes for CFTR detection, or boiled at 100 °C for 3 minutes for other protein detection. Total lysates of cells (20 µg per lane) or tissues (40 µg per lane) were then subjected to SDS–polyacrylamide gel electrophoresis and were transferred onto nitrocellulose membranes (HybondTM-P; Amersham Biosciences, Piscataway, NJ). The transferred membrane was blocked with TBS containing 0.2% Tween-20 (TBST) and 5% non-fat dry milk, and incubated with primary antibodies as follow: anti-CFTR (alomone labs ACL-006; US); anti-HIF-1α (Novus Biologicals NB100-449; US); EMT markers including anti-E-cadherin (Santa cruz sc-7870, clone H108; US), anti-Occludin (invitrogen 711500; US), anti-ZO-1 (invitrogen 617300, US), anti-Vimentin (Santa cruz sc-5565, clone H84; US), anti-N-cadherin (Invitrogen 333900; US) and anti-α-SMA (AbCAM ab5694; US), anti-Fibronectin (Santa Cruz sc-56391, clone SPM246; US), anti-Collagen1 (SouthernBiotech #1310-01; kindly from Prof Hui Yao LAN); Wnt/β-catenin signaling including Wnt signaling antibody sampler kit (Cell signaling technology 2915; US), Wnt/β-catenin activated targets antibody sampler kit (Cell signaling technology 8655; US), anti-β-catenin (Cell signaling technology 9562; US) at 4 °C overnight. Antibody against GAPDH (santa cruz sc-47724, clone 0411; US), β-tubulin (Santa Cruz sc-9104, clone 235; US) or Histone H1(Santa Cruz sc-8030, clone AE-4; US) were used for protein loading controls. The membrane was subsequently washed with TBST and incubated for 1 h with peroxidase-conjugated secondary antibodies. The membrane was washed three times with TBST and then detected by enhanced chemiluminescence (Amersham, Piscataway, NJ, USA).
Trans- epithelial electric resistance (TER)
MDCK cells were seeded into the 0.4 mm pore size transwell (upper chamber) and allowed to reach full confluence, after which fresh medium was replaced for further experiments. TER of epithelial monolayer was measured using EVOM electrovoltohmeter (WPI, Sarasota, FL) every two days following the standard procedure. Three replicated wells were used for measurement at each time point.
Wound-healing migration assay
Cells were seeded at 5 × 105 cells/well in 6-well plate one day before creating a wound across the cell monolayer with a plastic tip. Closure of the wound via the migration of cells was tracked and recorded using a Time Lapse Imaging System (CarlZwiss). Five 100× views from each well were documented and the results were expressed as reduced areas of wound. The experimental procedure was repeated three independent times.
Luciferase Activity Assay
The plasmids pcDNA3.1 and pcDNA3.1-CFTR were transiently co-transfected to HK-2 with TOPflash reporter gene (0.05 ug/ml) (Millipore, USA) by Lipofectamine 2000 (Invitrogen). Renilla-Luc (0.005 ug/ml) was included in all transfection assays as an internal control. Firefly and renilla luciferase activity was assessed with respective assay kit (Promega). The relative luciferase activity was defined as the ratio of readout for firefly luciferase to that for renilla luciferase with that of control group set as 1.0. Triplicate wells were used for measurement at each group. The experimental procedure was repeated three independent times.
Animals and Unilateral ureteric obstruction (UUO) model
All mice were provided by the Laboratory Animal Service Center of the Chinese University of Hong Kong. All animal experiments were conducted in accordance with the University Laboratory Animals Service Center’s guidelines on animal experimentation with approval from the Animal Ethnics Committee of the University. CFTR deltaF508 mouse model (The Jackson Laboratory, B6.129S6-Cftr tm1Kth) generated on C57BL/6 background41 was used in the study. KAPA Mouse Genotyping Kits (KAPA Biosystem) are used for genotyping followed standard protocol. Briefly, DNA was extracted from 3–5 mm mouse tail by the KAPA Express Extract (a thermostable protease and buffer system). The supernatant was then diluted 10-fold prior to use as template in PCR reaction (Figure S5b, Table S2). Homozygous mutant mice (deltaF508) were reduced in size compared to that of normal wild type mice (wt) and heterozygous (deltaF508 hetero). Mutant mice showed an increased mortality within the first month after birth (~60%), due to bowel obstructions, bowel strictures and peritonitis. Regardless of the survival rate, the animal lungs, pancreas, gall bladder, male reproductive tract, lacrimal gland and submandibular glands from deltaF508 mice appear normal.
Unilateral ureteric obstruction (UUO) model was established in female wild type and CFTR deltaF508 heterozygous (hetero), deltaF508 homozygous (homo); male wild type and smad3 knockout mice (provided by Prof. Hui Yao Lan, the Chinese University of Hong Kong) at 8-week-old. Six mice were used in each group. UUO model was established by left ureter ligation as previously described42. Mice were euthanized at Day 3 or 7, after the ligation. Kidney tissue samples were collected for histology, immunohistochemistry and real-time PCR.
The male C57 mice at 8-week-old were provided for the Ultrasound-mediated gene transfer within the kidney during fibrosis. The plasmid peGFP-C3 and peGFP-CFTR for injection were prepared using the EndoFree plasmid kit (Qiagen Inc., Valencia, CA, USA) according to the manufacturer’s instructions. Before injection, 200 μg of peGFP-C3 or peGFP-CFTR plasmid were mixed with sulphur hexafluoride microbubbles (Bracco Imaging B.V., Geneva, Switzerland) at a ratio of 1:1 (v/v). The mixed solution (200 μl) was injected into tail vein. Ultrasound was performed immediately after injection with a continuous-wave output of 1 MHZ for 5 min on the back of left kidney location. Unilateral ureteric obstruction (UUO) model was established following the ultrasound treatment. A group of 9 or 10 mice were euthanized at Day 7, after the ligation. Kidney tissue samples were collected for histology, immunohistochemistry, western blot and real-time PCR.
HE and Masson’s trichrome stain
Changes in renal morphology were examined in paraformaldehyde fixed, paraffin-embedded tissue sections (5 μm) stained with haematoxylin and eosin or Modified Masson’s Trichrome Stain Kit (ScyTek Laboratories). Briefly, deparaffinized sections were incubated in Bouin’s Fluid overnight. After wash by tap water, slides were stained sequentially by working Weigert’s Iron Hematoxylin for 5 mins, Biebrich Scarlet/Acid Fuchsin solution for 30 s, and Aniline Blue Solution for 45 mins.
Immunofluorescent and immunohistochemistry staining
MDCK or HK-2 cells were seeded into 24-well plate with cover slips for immunofluorescent assay. After treatment, cells were fixed with 4% paraformaldehyde and blocked with 1% bovine serum albumin, and incubated overnight with primary antibodies at a dilution of 1:100 in 1% BSA against ZO-1, Vimentin, N-cadherin, CFTR (Santa Cruz sc-8909, clone N-20; US) or Dvl2 at 4 °C. For paraffin embedded tissues, steps for rehydration and microwave-based antigen retrieval43 were performed before incubation with primary antibody against CFTR or HIF-1α. Then secondary Alexa fluor 568 goat anti-mouse IgG, fluor 488 goat anti-rabbit IgG, fluor 488 donkey anti-goat IgG or fluor 568 donkey anti-rabbit IgG at a dilution of 1:500 was loaded on cells or tissues for 1 hour. Samples were all co-stained with Hoechst for nuclei detection. Eventually, the slides were mounted with ProLong Gold Antifade Reagent (Invitrogen) and visualized with fluorescence microscope (Nikon Intersilight C-HGF1) using 4×, 10×, 20× and 40× objectives.
Immunohistochemistry was also performed in paraffin sections following the similar antigen retrieval procedure43. The primary antibodies used in this study include Collagen I and F4/80(AbD Serotec MCA497G, US). All slides were further stained with haematoxylin for the nuclei. 15 to 20 views were taken randomly by the Nikon 20× and 40× objectives of every slide for the quantitative analysis. Area of Collagen I staining was quantitated by the image analysis software (Meta Imaging series 7.5) on 200× fields. Briefly, the area of the positive staining pattern in tubulointerstitium was identified. Then, the percentage of positive area in the examined view was measured. The number of macrophages with the positive staining of F4/80 was counted in 400× fields, and the numbers were shown as cells/mm2. All analysis data was expressed as the Mean ± S.E.M. from three animals at indicated time point for each group.
All morphometric and staining data were collected blindly. Statistical significance for comparison between two measurements was determined using the unpaired Two-tailed Student t-test. One-way or Two-way ANOVA was used for evaluation of the significance among three or more measurements. All statistical analysis was completed by Prism 5 (GraphPad, Inc., San Diego, CA, USA). Values of p < 0.05 were regarded as statistically significant, and 0.01 < p < 0.05 was labeled as ‘*’, 0.001 < p < 0.01 was labeled as ‘**’ and p < 0.001 was labeled as ‘***’. All statistical data were shown as Mean ± S.E.M.
Downregulation of CFTR in murine and human fibrotic kidney
To test this hypothesis, we first examined whether CFTR expression was altered during the development of renal interstitial fibrosis by using unilateral ureteral obstruction (UUO) mouse model. Our western blot result showed that the expression of CFTR was dramatically decreased in fibrotic kidneys compared to normal kidneys (Fig. 1a). Consistently, while CFTR was extensively expressed on the apical surface of tubular lumen in the cortex and medulla in control kidney, UUO treatment drastically suppressed the expression level of CFTR (Fig. 1b). The expression of CFTR was further determined in normal and fibrotic human kidneys. In line with the mouse data, CFTR was expressed strongly at the apical membrane of tubular lumen in normal human kidney whereas dramatically downregulated in fibrotic kidney (Figs 1c and S1a). These data suggest that downregulation of CFTR is associated with the development of kidney fibrosis.
Hypoxia-downregulated CFTR induces upregulation of EMT markers independent of TGF-β
Next, we asked whether CFTR could be downregulated by factors known to induce EMT and kidney fibrosis. Since hypoxia is a major mediator of EMT during kidney fibrosis44, we investigated the influence of this pathophysiological important factor on the expression of CFTR in Madin-Darby canine kidney cell line MDCK and human tubular epithelial cell line HK-2, which are widely used as in vitro models to study EMT and renal dysfunction45. Our result showed that when subjected to hypoxia (1% O2), MDCK cells took on an elongated, fibroblastic morphology, which is reminiscent of a mesenchymal transition phenotype (Fig. 2a). Of note, the expression of CFTR was significantly downregulated in MDCK cells 48 hours after hypoxia treatment (Fig. 2b). The downregulation of CFTR was also observed in HK-2 cell by both real-time PCR and western blot analyses (Fig. 2c and d). In addition, the expression of hypoxia-inducible factor-1α (HIF-1α), a well-known critical mediator of hypoxia that has been identified as a negative regulator of CFTR39, was significantly increased along with alteration of EMT markers in the MDCK cells (Fig. 2e). To further correlate CFTR expression with hypoxia, we examined the expression of CFTR and HIF-1α in our UUO-treated mouse kidney tissues. Our results showed that the expression of HIF-1α was dramatically increased in the tubulointerstitial space at inner cortices 3 days after ligation in UUO mice, at which downregulation of CFTR was most significant (Fig. 2f). Taken together, downregulation of CFTR expression in both mouse and human kidney fibrotic tissues and downregulation of CFTR by hypoxia, along with upregulation of EMT markers suggest possible involvement of CFTR in EMT and progression of kidney fibrosis.
TGF-β has been considered as another major mediator of EMT in injured kidney41, 46. We further tested whether CFTR was involved in the TGF-β-induced EMT in renal epithelial cells. As shown in Supplementary Figure 1b, 5 ng/ml TGF-β1 readily induced EMT in MDCK cells as demonstrated by the significant upregulation of N-cadherin and Vimentin whereas downregulation of Occludin after 48–72 hours. However, we noticed that TGF-β1 did not have any effect on the expression level of CFTR. To further explore the possible correlation of TGF-β1 signaling with CFTR, we took advantage of Smad3 knockout (Smad3 KO) mouse model. Intriguingly, we found that the UUO treatment-induced effect on HIF-1α and CFTR was similar in both Smad3 KO mice and WT mice (Figure S1c), excluding the involvement of TGF- β/Smads pathway. We also determined the effect of CFTR inhibitors on TGF-β signaling and found that CFTR inhibitors did not affect the expression of phosphor-Smad2 (Figure S1d). Taken together, these data imply that CFTR downregulation during renal fibrosis is not related to TGF-β signaling.
Inhibition/knockdown of CFTR triggers EMT process
We further determined whether CFTR dysfunction or downregulation is a key event leading to EMT and kidney fibrosis. Firstly, we treated MDCK cells with two CFTR inhibitors, CFTRinh-172 (inh172) or GlyH101, and found marked morphological changes indicative of EMT in MDCK cells. CFTR inhibitor-treated cells had a greater predilection to break away as single cells and lose cell-cell contacts (Fig. 3a and Figure S2a). In addition, inhibition of CFTR significantly downregulated epithelial markers and upregulated mesenchymal markers in a time- and dose-dependent manner (Fig. 3b and Figure S2b). Moreover, dysfunction of CFTR led to disruption of tubular epithelial barrier integrity as evidenced by decreased transepithelial resistance (TER) (Fig. 3c) and enhanced migratory capability in wound healing assay (Fig. 3d). To further demonstrate the causative effect of CFTR downregulation on EMT induction, we knocked down CFTR in MDCK cells and found that CFTR silencing significantly decreased the expression levels of epithelial junction complex proteins, E-cadherin and ZO-1, whereas increased the mesenchymal markers, N-cadherin and Vimentin (Fig. 3e and Figure S2c). Similarly, knockdown of CFTR dramatically changed the expression of EMT markers including α-SMA and promoted cell migration in HK-2 cells (Figure S2d–g). In addition, fibrosis-focused PCR array illustrated that CFTR knockdown in HK-2 increased the expression of a variety of genes involved in EMT and matrix remodeling, such as COL1A2, COL3A1, MMPs, TIMP3 (Figure S3). These data indicate that suppression of CFTR function or expression is sufficient to trigger EMT process in renal tubular epithelial cells.
CFTR negatively regulates canonical Wnt/β-catenin signaling pathway
How does CFTR affect EMT and kidney fibrosis? Since over-activation of Wnt/β-catenin signaling has been implicated in the development of kidney fibrosis15, 17,18,19 and loss of CFTR has been shown to be associated with the activation of Wnt/β-catenin in intestinal epithelium and cancer35, 36, we hypothesized that CFTR might interfere with Wnt/β-catenin activation in kidney epithelial cells. Indeed, we found that suppression of CFTR function or expression increased the nuclear enrichment of β-catenin protein in HK-2 and MDCK cells (Fig. 4a and Figure S4a). Moreover, using TOPflash luciferase reporter assay, we illustrated that knockdown of CFTR increased β-catenin transcriptional activity whereas overexpression of CFTR decreased it (Fig. 4b and Figure S4b). Enhanced activation of β-catenin pathway was further validated by the observed increase in the expression levels of β-catenin target genes, which have been found transcriptionally regulated by β-catenin, such as axis inhibition protein 2 (AXIN2), tyrosine-protein kinase Met (Met), matrix metalloproteinases 7 (MMP7), MMP2 and G1/S-specific cyclin-D2 (cyclin D2) (Fig. 4c,d), but not the β-catenin destruction complex (Figure S4c) in CFTR-silenced HK-2 cells. Notably, the expression of MMP2 and MMP7, which are involved in EMT process and kidney fibrosis47, was significantly upregulated by CFTR knockdown. We next asked whether CFTR downregulation-induced over-activation of β-catenin pathway was responsible for the observed EMT process. We first treated HK-2 cells with GSK3 inhibitor, CHIR99021 to activate β-catenin, and determined the expression of EMT markers. The results showed that activation of β-catenin significantly downregulated epithelial markers E-cadherin and ZO-1, whereas increased the expression of α-SMA (Figure S4d). Next, we asked whether the effects observed with CFTR knockdown could be reversed by inhibiting β-catenin activity. To test this, we treated CFTR knockdown HK-2 cells with a β-catenin-responsive transcription inhibitor, iCTR14 and the results showed that it reversed the CFTR knockdown-induced decrease of E-cadherin (Fig. 4e), suggesting the involvement of β-catenin signaling in the EMT process induced by CFTR suppression.
CFTR regulates β-catenin signaling via its interaction with Dvl2
How does CFTR regulate β-catenin signaling? The cytoplasmic protein Dvl, which functions as an adaptor protein of the Wnt/β-catenin pathway, has a PDZ domain17, 21. Of interest, CFTR contains a PDZ-binding domain at its carboxy terminus, which has been shown to interact with the PDZ domain of a large variety of proteins48. Thus, we hypothesized that CFTR might interact with Dvl through the PDZ domain, thereby interfering with Wnt/β-catenin activation. We first checked whether CFTR and Dvl2, the most prominent isoform of Dvls expressed in mammals, might interact with each other in HK-2 cells. Our results showed that CFTR co-localized with Dvl2 at the cytoplasm (Fig. 4f) and physically interacted with Dvl2 as demonstrated by co-immunoprecipitation (co-IP) assay (Fig. 4g). To determine whether the interaction of CFTR and Dvl2 is dependent on the PDZ-binding domain of CFTR, which consists of four amino acids DTRL at the C-terminus, plasmids expressing full length CFTR (WT) or CFTR (ΔPDZ) (full length CFTR depleting DTRL) were transfected into HEK-293 cells, and the interaction between WT CFTR or CFTRΔPDZ with Dvl2 was determined by co-IP. Our results showed that the interaction between CFTR and Dvl2 could only be detected in WT CFTR- but not CFTRΔPDZ transfected-cells (Fig. 4h), suggesting that the physical interaction between CFTR and Dvl2 is via PDZ-binding motif. We further determined whether deletion of PDZ binding motif would affect the β-catenin transcriptional activity by using TOPflash luciferase reporter assay. Consistent with our co-IP assay, transfection of full length CFTR but not ΔPDZ dramatically suppressed β-catenin activity in HK-2 cells (Fig. 4i and Figure S4e). Taken together, these results suggest that by interacting with Dvl2, CFTR negatively regulates canonical Wnt/β-catenin signaling pathway in tubular epithelial cells.
Disruption of CFTR exacerbates fibrotic phenotype in UUO model
Next, we investigated whether CFTR defect could influence the development of kidney fibrosis in vivo. We examined the progression of obstructive renal fibrogenesis in deltaF508 CFTR mutant mice, a well-established animal model with a mutation found in majority of CF patients49. The results showed that after UUO, homozygous (deltaF508 homo) mice, developed more intensive tubule-interstitial damage including tubular atrophy, inflammatory cell invasion and interstitial extracellular matrix accumulation as demonstrated by Masson trichrome staining, collagen I deposition and macrophage infiltration in the fibrotic area of tubule-interstitium (Fig. 5a,b, Figure S5). These findings indicate that the dysfunction of CFTR leads to enhanced tubulointerstitial injury and fibrosis in vivo. Consistent with the in vitro data, the obstructive injury led to a dramatic accumulation of β-catenin in the deltaF508 mice compared to the wild type mice (Fig. 6a). Moreover, higher expression levels of Wnt target genes, such as Axin2, c-jun and Mmp7, were observed in the UUO-treated deltaF508 mice than that in the UUO-treated wild type mice (Fig. 6b–d), indicating aberrant activation of the canonical Wnt signaling pathway in CFTR mutant mice.
Overexpression of CFTR alleviates fibrotic phenotype in UUO model
We next tested whether forced introduction of CFTR could alleviate the progression of renal fibrogenesis by using ultrasound-microbubble technique50 to deliver CFTR DNA to UUO kidneys. CFTR plasmids were delivered to the mouse kidney before the initiation of UUO procedure, and the effects of CFTR overexpression was examined 7 days after UUO treatment. Our results showed that the expression of interstitial matrix related genes such as FN, Col1a1 and α-SMA was significantly decreased at both mRNA and protein levels in CFTR-overexpressing obstructive kidneys compared to that in the vector control group after UUO (Fig. 6e–h and Figure S6a), indicating that overexpression of CFTR significantly retarded the fibrotic process. In addition, forced introduction of CFTR significantly reduced the expression of β-catenin target gene, Axin2 in UUO kidney (Figure S6b). Collectively, these results suggest that disruption of the CFTR gene exacerbates fibrotic phenotype whereas overexpression of CFTR alleviates it in the UUO model.
The present results have revealed a previously unrecognized role of CFTR in the development of EMT and kidney fibrosis via its regulation on β-catenin signaling pathway. In the study, we have found that suppression of CFTR downregulates the expression of Occludin and ZO-1, which are tight junction proteins, in tubular epithelial cells. This finding provides a plausible mechanism for the long-standing observation that CF patients have excessive leakage of low molecular weight (LMW) protein in the urine51. In addition, the demonstrated aggravation of UUO-induced EMT program and renal fibrogenesis in CFTR mutant mice may explain the observation that chronic kidney diseases are commonly seen in CF patients29.
Over-activation of β-catenin pathway has been shown to be associated with the development of kidney fibrosis and chronic kidney diseases15, 52, 53. Originally, it was reported that most of the 19 Wnt proteins and 10 Fzd receptors were increased in renal tubular cells in the UUO model of kidney fibrosis15. Subsequent mechanistic studies further indicated that Wnt and β-catenin were not only regulated in chronic kidney diseases, but also contributed to fibrosis development15. For instance, genetic overexpression of active β-catenin in tubular cells induced fibrosis feature, including epithelial dedifferentiation and EMT in mice52, 53. Most importantly, it has been shown recently that suppression of Wnt/β-catenin signaling, by either targeting upstream signaling factors or downstream targets ameliorates inflammation and tubulointerstitial fibrosis in mouse models54, 55. In the present study, we have clearly shown that β-catenin pathway is upregulated in both CFTR- knockdown renal epithelial cells and UUO-treated kidney of deltaF508 mice (Figs 4a–d and 6a). In addition, β-catenin activator promoted EMT process, whereas β-catenin inhibitor reversed the CFTR knockdown-induced decrease of E-cadherin (Figure S4d and Fig. 4e). These results clearly indicate that CFTR negatively regulates β-catenin pathway in renal epithelial cells. In strikingly contrast, our recent studies reported that CFTR positively regulated β-catenin activity in the intestinal epithelial cells and embryonic stem cells35, 56. In both studies, CFTR was shown to physically interact with β-catenin, and defect of CFTR led to decreased β-catenin-mediated signaling. Of note, in this study, we did not find interaction between CFTR and β-catenin in kidney epithelial cells and kidney tissues. Instead, we found that CFTR interacted with Dvl, a key adaptor in Wnt/β-catenin signaling, and that this interaction appeared to be important for suppressing the Wnt/β-catenin signaling. In canonical Wnt signaling, Dvl functions as a scaffold protein bridging Wnt receptors and downstream signaling components via its PDZ domain57. In the present study, apart from Dvl2, we did not find interaction of CFTR with other β-catenin signaling components, such as Axin2 and β-catenin, in HK-2 cells (data not shown). While we cannot entirely rule out other possibilities, these results suggest that the interaction between CFTR and Dvl via PDZ domain is likely to contribute to the effect of CFTR on β-catenin activity in kidney epithelial cells. As a result, disruption of the interaction due to loss of CFTR results in aberrant activation of the Wnt/β-catenin-dependent EMT, leading to renal fibrosis. Of note, CFTR has also been found to negatively regulate the expression and activation of β-catenin in colon crypt cells and lung epithelial cells36, 58. Taken together, it appears that CFTR may exert differential effect on β-catenin signaling in different cellular contexts, probably via interacting with different components of the Wnt/β-catenin pathway.
Decreases in tissue oxygen levels are commonly associated with injury and repair44. As CFTR is expressed in a large variety of tissues, the presently observed hypoxia-induced CFTR downregulation provides a missing link between micro-environmental signaling i.e. hypoxia, and intracellular signaling pathways, i.e. Wnt/β-catenin, leading to EMT process during physiological and/or pathological conditions, such as fibrosis and cancer. On the other hand, it is very likely that dysfunction of CFTR itself augments the pathological process of kidney fibrosis in response to certain hostile environment, such as hypoxia and high glucose in CF patients. It should be noted that previous studies have established a link between TGF-β pathway and β-catenin pathway in the context of renal diseases54, 59. For instance, Takenaka et al., showed that klotho protein suppressed epithelial-mesenchymal transition in Adriamycin-induced nephropathy by inhibiting TGF-β and Wnt signaling59. However, in our study, we clearly showed that TGF-β, one of the key mediators of tissue fibrosis did not affect CFTR expression. Moreover, UUO-induced downregulation of CFTR was also observed in Smad3 KO mice, excluding the involvement of TGF-β signaling since Smad3 is a key player of the TGF-β signaling60. These results indicate that the TGF-β pathway is dissociated with the CFTR-mediated regulatory pathway in the development of hypoxia-induced renal fibrosis. Indeed, while TGF-β1 inhibitors have been used in the clinic to treat chronic kidney diseases; the therapeutic effect is not significant61,62,63. This observation indicates other potential players might be involved. The presently observed downregulation of CFTR expression in human fibrotic kidneys and the suppression of kidney fibrosis progression in UUO mice upon forced CFTR overexpression indicate the possibility of using CFTR as a new or combined treatment target along with TGF-β in the fibrotic kidney diseases, which warrants future investigations.
Carew, R. M., Wang, B. & Kantharidis, P. The role of EMT in renal fibrosis. Cell and tissue research 347, 103–116, doi:10.1007/s00441-011-1227-1 (2012).
Gilbert, R. E. & Cooper, M. E. The tubulointerstitium in progressive diabetic kidney disease: more than an aftermath of glomerular injury? Kidney international 56, 1627–1637, doi:10.1046/j.1523-1755.1999.00721.x (1999).
Nath, K. A. Tubulointerstitial changes as a major determinant in the progression of renal damage. American journal of kidney diseases: the official journal of the National Kidney Foundation 20, 1–17 (1992).
Strutz, F. The fibroblast–a (trans-) differentiated cell? Nephrol Dial Transplant 10, 1504–1506 (1995).
Zeisberg, M., Maeshima, Y., Mosterman, B. & Kalluri, R. Renal fibrosis. Extracellular matrix microenvironment regulates migratory behavior of activated tubular epithelial cells. Am J Pathol 160, 2001–2008, doi:10.1016/S0002-9440(10)61150-9 (2002).
Iwano, M. et al. Evidence that fibroblasts derive from epithelium during tissue fibrosis. J Clin Invest 110, 341–350, doi:10.1172/JCI15518 (2002).
Kalluri, R. & Neilson, E. G. Epithelial-mesenchymal transition and its implications for fibrosis. J Clin Invest 112, 1776–1784, doi:10.1172/JCI20530 (2003).
Kim, M. K. et al. The differential expression of TGF-beta1, ILK and wnt signaling inducing epithelial to mesenchymal transition in human renal fibrogenesis: an immunohistochemical study. International journal of clinical and experimental pathology 6, 1747–1758 (2013).
LeBleu, V. S. et al. Origin and function of myofibroblasts in kidney fibrosis. Nature medicine 19, 1047–1053, doi:10.1038/nm.3218 (2013).
Hills, C. E. & Squires, P. E. The role of TGF-beta and epithelial-to mesenchymal transition in diabetic nephropathy. Cytokine & growth factor reviews 22, 131–139, doi:10.1016/j.cytogfr.2011.06.002 (2011).
Kriz, W., Kaissling, B. & Le Hir, M. Epithelial-mesenchymal transition (EMT) in kidney fibrosis: fact or fantasy? J Clin Invest 121, 468–474 (2011).
Liu, Y. New insights into epithelial-mesenchymal transition in kidney fibrosis. Journal of the American Society of Nephrology: JASN 21, 212–222, doi:10.1681/ASN.2008121226 (2010).
Iglesias, D. M. et al. Canonical WNT signaling during kidney development. American journal of physiology. Renal physiology 293, F494–500, doi:10.1152/ajprenal.00416.2006 (2007).
Peng, J. & Dong, Z. Role changes of beta-catenin in kidney injury and repair. Kidney international 82, 509–511, doi:10.1038/ki.2012.155 (2012).
He, W. et al. Wnt/beta-catenin signaling promotes renal interstitial fibrosis. Journal of the American Society of Nephrology: JASN 20, 765–776, doi:10.1681/ASN.2008060566 (2009).
Surendran, K., McCaul, S. P. & Simon, T. C. A role for Wnt-4 in renal fibrosis. American journal of physiology. Renal physiology 282, F431–441, doi:10.1152/ajprenal.0009.2001 (2002).
Surendran, K., Schiavi, S. & Hruska, K. A. Wnt-dependent beta-catenin signaling is activated after unilateral ureteral obstruction, and recombinant secreted frizzled-related protein 4 alters the progression of renal fibrosis. Journal of the American Society of Nephrology: JASN 16, 2373–2384, doi:10.1681/ASN.2004110949 (2005).
Zhou, T. et al. Implication of dysregulation of the canonical wingless-type MMTV integration site (WNT) pathway in diabetic nephropathy. Diabetologia 55, 255–266, doi:10.1007/s00125-011-2314-2 (2012).
Xiao, L., Wang, M., Yang, S., Liu, F. & Sun, L. A glimpse of the pathogenetic mechanisms of Wnt/beta-catenin signaling in diabetic nephropathy. BioMed research international 2013, 987064, doi:10.1155/2013/987064 (2013).
Dai, C. et al. Wnt/beta-catenin signaling promotes podocyte dysfunction and albuminuria. Journal of the American Society of Nephrology: JASN 20, 1997–2008, doi:10.1681/ASN.2009010019 (2009).
von Toerne, C. et al. Wnt pathway regulation in chronic renal allograft damage. American journal of transplantation: official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 9, 2223–2239, doi:10.1111/j.1600-6143.2009.02762.x (2009).
He, W. et al. Matrix metalloproteinase-7 as a surrogate marker predicts renal Wnt/beta-catenin activity in CKD. Journal of the American Society of Nephrology: JASN 23, 294–304, doi:10.1681/ASN.2011050490 (2012).
Hao, S. et al. Targeted inhibition of beta-catenin/CBP signaling ameliorates renal interstitial fibrosis. Journal of the American Society of Nephrology: JASN 22, 1642–1653, doi:10.1681/ASN.2010101079 (2011).
He, W. et al. Plasminogen activator inhibitor-1 is a transcriptional target of the canonical pathway of Wnt/beta-catenin signaling. The Journal of biological chemistry 285, 24665–24675, doi:10.1074/jbc.M109.091256 (2010).
Riordan, J. R. The molecular biology of chloride channels. Current opinion in nephrology and hypertension 1, 34–42 (1992).
Riordan, J. R. et al. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245, 1066–1073 (1989).
Collins, F. S. Cystic fibrosis: molecular biology and therapeutic implications. Science 256, 774–779 (1992).
Morales, M. M., Falkenstein, D. & Lopes, A. G. The cystic fibrosis transmembrane regulator (CFTR) in the kidney. Anais da Academia Brasileira de Ciencias 72, 399–406 (2000).
Nazareth, D. & Walshaw, M. A review of renal disease in cystic fibrosis. Journal of cystic fibrosis: official journal of the European Cystic Fibrosis Society 12, 309–317, doi:10.1016/j.jcf.2013.03.005 (2013).
Plant, B. J., Goss, C. H., Plant, W. D. & Bell, S. C. Management of comorbidities in older patients with cystic fibrosis. Lancet Respir Med 1, 164–174, doi:10.1016/S2213-2600(13)70025-0 (2013).
Koch, C. et al. Presence of cystic fibrosis-related diabetes mellitus is tightly linked to poor lung function in patients with cystic fibrosis: data from the European Epidemiologic Registry of Cystic Fibrosis. Pediatric pulmonology 32, 343–350 (2001).
Guo, J. H. et al. Glucose-induced electrical activities and insulin secretion in pancreatic islet beta-cells are modulated by CFTR. Nat Commun 5, 4420, doi:ncomms5420 (2014).
O’Connell, O. J., Harrison, M. J., Murphy, D. M., Plant, W. D. & Plant, B. J. Peri-lung transplant renal issues in patients with cystic fibrosis. Chest 143, 271, doi:10.1378/chest.12-2057 (2013).
Pankow, S. et al. F508 CFTR interactome remodelling promotes rescue of cystic fibrosis. Nature 528, 510–516, doi:10.1038/nature15729 (2015).
Liu, K. et al. Defective CFTR- beta-catenin interaction promotes NF-kappaB nuclear translocation and intestinal inflammation in cystic fibrosis. Oncotarget 7, 64030–64042, doi:10.18632/oncotarget.11747 (2016).
Than, B. L. et al. CFTR is a tumor suppressor gene in murine and human intestinal cancer. Oncogene 35, 4179–4187, doi:10.1038/onc.2015.483 (2016).
Pruliere-Escabasse, V. et al. TGF-beta 1 downregulates CFTR expression and function in nasal polyps of non-CF patients. Am J Physiol Lung Cell Mol Physiol 288, L77–83, doi:10.1152/ajplung.00048.2004 (2005).
Howe, K. L., Wang, A., Hunter, M. M., Stanton, B. A. & McKay, D. M. TGFbeta down-regulation of the CFTR: a means to limit epithelial chloride secretion. Exp Cell Res 298, 473–484, doi:10.1016/j.yexcr.2004.04.026 (2004).
Zheng, W. et al. Hypoxia inducible factor-1 (HIF-1)-mediated repression of cystic fibrosis transmembrane conductance regulator (CFTR) in the intestinal epithelium. FASEB journal: official publication of the Federation of American Societies for Experimental Biology 23, 204–213, doi:10.1096/fj.08-110221 (2009).
Xie, C. et al. CFTR suppresses tumor progression through miR-193b targeting urokinase plasminogen activator (uPA) in prostate cancer. Oncogene 32, 2282–2291, doi:10.1038/onc.2012.251 (2013). 2291.e2281–2287.
Zeiher, B. G. et al. A mouse model for the delta F508 allele of cystic fibrosis. J Clin Invest 96, 2051–2064 (1995).
Lan, H. Y. et al. Inhibition of renal fibrosis by gene transfer of inducible Smad7 using ultrasound-microbubble system in rat UUO model. Journal of the American Society of Nephrology: JASN 14, 1535–1548 (2003).
Huang, X. R., Chung, A. C., Zhou, L., Wang, X. J. & Lan, H. Y. Latent TGF-beta1 protects against crescentic glomerulonephritis. Journal of the American Society of Nephrology: JASN 19, 233–242, doi:10.1681/ASN.2007040484 (2008).
Nangaku, M. Chronic hypoxia and tubulointerstitial injury: a final common pathway to end-stage renal failure. Journal of the American Society of Nephrology: JASN 17, 17–25, doi:10.1681/ASN.2005070757 (2006).
Park, S. H. et al. Erythropoietin decreases renal fibrosis in mice with ureteral obstruction: role of inhibiting TGF-beta-induced epithelial-to-mesenchymal transition. Journal of the American Society of Nephrology: JASN 18, 1497–1507, doi:10.1681/ASN.2005080866 (2007).
Böttinger, E. P. & Bitzer, M. TGF-ß signaling in renal disease. Journal of the American Society of Nephrology 13, 2600–2610 (2002).
Zhao, H. et al. Matrix metalloproteinases contribute to kidney fibrosis in chronic kidney diseases. World journal of nephrology 2, 84–89, doi:10.5527/wjn.v2.i3.84 (2013).
Moyer, B. D. et al. A PDZ-interacting domain in CFTR is an apical membrane polarization signal. J Clin Invest 104, 1353–1361, doi:10.1172/JCI7453 (1999).
French, P. J. et al. A delta F508 mutation in mouse cystic fibrosis transmembrane conductance regulator results in a temperature-sensitive processing defect in vivo. J Clin Invest 98, 1304–1312, doi:10.1172/JCI118917 (1996).
Hou, C. C. et al. Ultrasound-microbubble-mediated gene transfer of inducible Smad7 blocks transforming growth factor-beta signaling and fibrosis in rat remnant kidney. Am J Pathol 166, 761–771 (2005).
Jouret, F. et al. Cystic fibrosis is associated with a defect in apical receptor-mediated endocytosis in mouse and human kidney. Journal of the American Society of Nephrology: JASN 18, 707–718, doi:10.1681/ASN.2006030269 (2007).
Zhou, D. et al. Tubule-specific ablation of endogenous beta-catenin aggravates acute kidney injury in mice. Kidney international 82, 537–547, doi:10.1038/ki.2012.173 (2012).
Zhou, D., Tan, R. J., Zhou, L., Li, Y. & Liu, Y. Kidney tubular beta-catenin signaling controls interstitial fibroblast fate via epithelial-mesenchymal communication. Sci Rep 3, 1878, doi:10.1038/srep01878 (2013).
Chen, C. H. et al. Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway. Sci Rep 7, 40183, doi:10.1038/srep40183 (2017).
Johnson, B. G. et al. Connective Tissue Growth Factor Domain 4 Amplifies Fibrotic Kidney Disease through Activation of LDL Receptor-Related Protein 6. Journal of the American Society of Nephrology: JASN. doi:10.1681/asn.2016080826 (2017).
Liu, Z. et al. CFTR-beta-catenin interaction regulates mouse embryonic stem cell differentiation and embryonic development. Cell death and differentiation 24, 98–110, doi:10.1038/cdd.2016.118 (2017).
Wong, H. C. et al. Direct binding of the PDZ domain of Dishevelled to a conserved internal sequence in the C-terminal region of Frizzled. Molecular cell 12, 1251–1260 (2003).
Cohen, J. C., Larson, J. E., Killeen, E., Love, D. & Takemaru, K. CFTR and Wnt/beta-catenin signaling in lung development. BMC developmental biology 8, 70, doi:10.1186/1471-213x-8-70 (2008).
Takenaka, T. et al. Klotho suppresses the renin-angiotensin system in adriamycin nephropathy. Nephrol Dial Transplant, doi:10.1093/ndt/gfw340 (2016).
Sato, M., Muragaki, Y., Saika, S., Roberts, A. B. & Ooshima, A. Targeted disruption of TGF-β1/Smad3 signaling protects against renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction. The Journal of clinical investigation 112, 1486–1494 (2003).
Noble, P. W. et al. Pirfenidone in patients with idiopathic pulmonary fibrosis (CAPACITY): two randomised trials. Lancet 377, 1760-1769, doi:S0140-6736(11)60405-4 (2011).
Armendariz-Borunda, J. et al. A pilot study in patients with established advanced liver fibrosis using pirfenidone. Gut 55, 1663–1665, doi:10.1136/gut.2006.107136 (2006).
Walker, J. E., Giri, S. N. & Margolin, S. B. A double-blind, randomized, controlled study of oral pirfenidone for treatment of secondary progressive multiple sclerosis. Mult Scler 11, 149–158, doi:10.1191/1352458505ms1134oa (2005).
The work was supported in parts by National 973 project (2013CB967404, 2013CB967401, 2013CB967403), Research Grant Council of Hong Kong (CUHK 466413, CUHK14119516), National Natural Science Foundation of China (81571390), the Focused Investment Scheme of the Chinese University of Hong Kong and L.K.S Foundation.
The authors declare that they have no competing interests.
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Zhang, J.T., Wang, Y., Chen, J.J. et al. Defective CFTR leads to aberrant β-catenin activation and kidney fibrosis. Sci Rep 7, 5233 (2017). https://doi.org/10.1038/s41598-017-05435-5
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