Diterpenoid Alkaloids from Delphinium anthriscifolium var. majus

Abstract

Extensive phytochemical investigation on the whole herbs of Delphinium anthriscifolium var. majus led to the identification of fourteen diterpenoid alkaloids, including three new C₂₀–diterpenoid alkaloids (anthriscifolsines A–C, 1–3), six new C₁₉–diterpenoid alkaloids (anthriscifolrines A–F, 4–9), and five know compounds (10–14). Among them, anthriscifolsine A represents a novel C₂₀–diterpenoid alkaloid characterized by a seco C–ring. The structures of the isolated compounds were elucidated by extensive spectroscopic methods, including HR-ESI–MS, X–ray, and 1D and 2D NMR experiments. Bioactivity of compounds 3–6 was evaluated for their cytotoxicity against the MCF–7, HepG2 and H460 cancer cell lines.

Introduction

Delphinium is a large genus comprising 350 species and distributed in the temperate regions of the Northern Hemisphere, of which 173 are found in mainland China¹. In our continuous phytochemical studies on the pharmacologically interesting plants of the genera Aconitum and Delphinium, we obtained a series of structurally and chemotaxonomically diverse diterpenoid alkaloids^2,3,4,5. Delphinium anthriscifolium var. majus is an herbaceous plant, belonging to the Sect. Anthriscifolium of the genus Delphinium, and widely distributed in Guizhou, Sichuan, Hubei and Shanxi provinces in China⁶. Our earlier chemical investigation of this plant led to the discovery of two new C₁₈–diterpenoid alkaloids². Further studies on the whole extract of this plant resulted in the isolation and structural determination of three new C₂₀–diterpenoid alkaloids, anthriscifolsines A–C (1–3), six new C₁₉–diterpenoid alkaloids, anthriscifolrines A–F (4–9) (Fig. 1), and five know alkaloids nudicaulamine (10)⁷, anthriscifolmine C (11)⁸, anthriscifolmine D (12)⁹, anthriscifolmine I (13)¹⁰, and hetisine 13–O–acetate (14)¹¹. Anthriscifolsine A represents a new type of C₂₀–diterpenoid alkaloid, featuring a seco C–ring through an unprecedented C11–C12 bond cleavage of hetisine-type skeleton, whose stereostructure has been unambiguous established by an X–ray crystallographic analysis. Cytotoxicity of diterpenoid alkaloids against MCF–7, HepG2 and H460 cancer cell lines was also evaluated by the MTT method. Herein, we report the isolation, structural elucidation and bioactivity of these diterpenoid alkaloids.

Figure 1

Structures of compounds 1–14.

Results

Anthriscifolsine A (1) was obtained as needles via crystallization from MeOH. Its molecular formula, C₂₉H₃₁NO₇, was deduced from the HR–ESI–MS (m/z 506.2182 [M + H]⁺, calcd for C₂₉H₃₂NO₇, 506.2179) and ¹³C NMR spectroscopic data. The ¹H NMR data (Table 1) displayed characteristic resonances of two methyl [δ _H 1.10, 1.97 (each 3 H, s)], an acetyl [δ _H 2.29 (3 H, s)], a benzoyl [δ _H 7.32 (2 H, t, J = 7.8 Hz), 7.51 (1 H, t, J = 7.8 Hz), 7.89 (2 H, d, J = 7.8 Hz)], and an aldehyde [δ _H 9.84 (1 H, br.s)] groups. The ¹³C NMR and DEPT spectra of 1 exhibited the presence of two methyls (δ _C 18.8, 24.6), three methylenes (δ _C 33.2, 33.5, 36.3), eight sp³ methines (δ _C 54.9, 58.5, 59.1, 61.1, 63.9, 67.3, 74.3, 88.4), and three sp³quaternary carbons (δ _C 44.4, 47.1, 47.6), one trisubstituted double bond (δ _C 124.6, 158.5), one aldehyde (δ _C 201.0), one keto carbonyl (δ _C 196.1). In addition, an acetyl group [δ _C 21.6 (q), 169.8 (s)], a benzoyl group [δ _C 129.8 (s), 129.7 × 2 (d), 128.4 × 2 (d), 133.2 (d), 165.5 (s)] were presented in the structure according to the NMR spectra. These characteristic spectroscopic data suggested that 1 was a typical skeleton of C₂₀–diterpenoid alkaloid diester⁹. The proton and corresponding carbon resonances in the 2D NMR spectra of 1 were assigned by the HMQC experiment. The existence of three oxygenated carbons deduced from its ¹³C NMR spectrum suggests that 1 has a hydroxyl group, in addition to two ester groups. The absence of a typical C–19 methylene signals in its NMR spectra suggested that a hydroxyl group might be located at C–19, which was confirmed by the HMBC correlations (Fig. 2) from H–3, H₃–18 and H–5 to C–19⁹. The acetoxy group could be assigned to C–2 and the benzoyl group at C–3 respectively, on the basis of the HMBC correlations from H–2 (δ _H 5.59, m) to the carbonyl carbon of the acetyl group at δ _C 169.8 and H–3 (δ _H 5.18, d, J = 4.8 Hz) to the carbonyl carbon at δ _C 165.5 of the benzoyl group. Compound 1 has the same macular formula and similar NMR spectraoscopic data with those of anthriscifolmine C (11)⁹, which also possesses an acetyl group at C–2 and a benzoyl group at C–3. However, compound 1 differs from anthriscifolmine C (11) mainly at C–11 where an aldehyde group and a trisubstituted double bond between C–12 and C–16 were deduced. Two methys group were shown to be attached at C–4 and C–16 according to the HMBC correlations from H₃–18 to C–3, C–4, C–5 and C–19, and H₃–17 to C–12, C–15 and C–16. The substitution pattern and the assigned planar structure of 1 were confirmed by complete ¹H−¹H COSY and HMBC spectroscopic analysis.

Table 1 NMR Spectroscopic Data^a for Compounds 1–3 (600 MHz for ¹H, 150 MHz for ¹³C, CDCl₃, δ ppm).

Figure 2

Key HMBC and ¹H–¹H COSY interactions of compounds 1–3.

The NOESY correlations of H–1β and H–3, H–3 and H–5, H–1α and H–11, H–1α and H–20, H–19 and H–20, proved that H–3 was β–oriented, H–11 and H–19 were in α–orientation (Fig. 3). The NOESY correlations indicated that H–2 was in an equatorial position, which indicated a β–orientation. Moreover, an X–ray diffraction experiment with a suitable crystal was conducted and the absolute configuration of 1 was established as H–2β, H–3β, H–11α, H–19α (19–s) (Fig. 4), consistent with the absolute configuration determined by NOESY correlations. Thus, the structure of 1 was assigned as shown in Fig. 1.

Figure 3

Key NOESY correlations of compounds 1–3.

Figure 4

ORTEP projection of compound 1 (crystallographic numbering).

A possible biogenetic pathway of anthriscifolsine A (1) was proposed as shown in Fig. 5. Aldehyde A could be generated from the known alkaloid anthriscifolmine C (11) through a critical retro–aldol process involving the cleavage of C11–C12 bond. The latter has been also isolated from this plant, which was obtained as needles crystal (MeOH), and the structure of which was unambiguously confirmed by an X–ray crystallographic analysis (Fig. 6). The unstable intermediate A then underwent proton shift and epimerization of the C9 stereochemistry, thus leading to anthriscifolsine A (1). Finally, the artificial possibility of anthriscifolsine A (1) had been explicitly excluded used UPLC–HRESI–MS method and the detailed experiments were added the Supporting Information.

Figure 5

Postulated biogenetic pathway of anthriscifolsine A (1).

Figure 6

ORTEP projection of compound 11 (crystallographic numbering).

Anthriscifolsine B (2) was obtained as a white amorphous powder. Its molecular formula C₂₄H₃₁NO₇ was derived from a pseudomolecular ion at m/z 446.2196 [M + H]⁺ in its HR–ESI–MS. It exhibited characteristic NMR features of a hetisine–type C₂₀–diterpenoid alkaloid bearing groups including two acetyl groups, and an exocyclic double bond (Table 1)¹². Two acetyl groups can be installed at C–2 and C–3, respectively, on the basis of the HMBC correlations from H–2 (δ _H 5.35, m) to the carbonyl carbon of one acetyl group at δ _C 170.2 and H–3 (δ _H 4.93, d, J = 4.8 Hz) to the carbonyl carbon of another acetyl group at δ _C 170.6. Along with the abovementioned signals, its ¹³C NMR spectrum displayed five oxygenated carbon signals, suggesting that this compound possessed three additional hydroxyl groups in addition to two ester groups. Two hydroxyl groups were assigned at C–11 and C–13 based on the HMBC correlations from H–11 to C–10, C–13 and C –16, and H–13 to C –11, and COSY correlations of H–11/H–12/H–13/H–14. The observation of HMBC crosspeak between C–9 (δ _C 80.2) and H–1 (δ _H 1.99), H–7(δ _H 1.39), H–12 (δ _H 2.51), and H–20 (δ _H 3.71), facilitated the location of the third hydroxyl group at C–9.

The relative configuration of 2 was deduced from the vicinal coupling constants and a NOESY experiment (Fig. 3). The coupling constant (J = 4.8 Hz) of H–2 with H–3, indicated that H–2 was in an equatorial position, which indicated a β–orientation⁹. The large coupling constant of H–13 (J = 8.4 Hz) with H–14α revealed that the dihedral angle between these two H–atoms was ca. 0 °C, which implied that H–13 was in an α–orientation⁹. In the NOESY spectrum of 2, the cross–peak between H–1β and H–3, H–3 and H–5, H–1α and H–11, H–1α and H–20, proved that H–3 was β–oriented and H–11was in α–orientation. Therefore, the structure of anthriscifolsine B was determined as shown in Fig. 1, and the full assignment of its spectroscopic data was achieved based on the 1D– and 2D NMR analysis (Table 1, Fig. 2).

Anthriscifolsine C (3) was isolated as a white amorphous powder and its molecular formula was deduced to be C₃₀H₄₃NO₇ by HR–ESI–MS at m/z 530.3118 [M + H]⁺. The ¹H NMR and ¹³C NMR data (Table 1) of 3 indicated the presence of the signals of two 2–methylbutanoyloxy groups (MbO) at [(δ _H 2.44 (1 H, m), 1.73 (2 H, m), 0.91(3 H, t, J = 7.2 Hz), 1.20 (3 H, d, J = 7.2 Hz) and δ _C 177.3 (s), 41.9 (d), 26.8 (t), 11.9 (q), 16.8 (q)] and [(δ _H 2.40 (1 H, m), 1.50 (2 H, m), 0.93 (3 H, t, J = 7.2 Hz), 1.16 (3 H, d, J = 7.2 Hz) and δ _C 176.8 (s), 41.6 (d), 26.7 (t), 11.8 (q), 17.0 (q)]². The remaining 20 carbons were assigned based on 1D– and 2D–NMR data and exhibited characteristic NMR features of a hetisine–type C₂₀–diterpenoid alkaloid¹² bearing five methylenes, nine methines (four oxygenated) and five quaternary carbons (one ester carbonyl), in addition to one methyl group that was attached to a quaternary carbon (Table 1). The presence of an exocyclic double bond was evidenced by singals in the ¹H NMR spectrum (δ _H 4.87, d, J = 1.8 Hz, 4.98, d, J = 1.8 Hz) and ¹³C NMR spectrum (δ _C 111.2 and 148.5). The locations of two 2–methylbutanoyloxy groups at C–2 and C–15 were determined by the correlations in the HMBC experiment (Fig. 2). The ¹³C NMR spectrum showed a singlet at δ _C 97.0, indicative of a carbinolamine carbon (C–6). Besides the two ester groups and the carbinolamine carbon, there were two OH groups in the molecule, which were placed at C–3 and C–11, respectively, according to the HMBC displayed in Fig. 2. The coupling constant (J = 5.4 Hz) of H–2 with H–3 indicated that H–2 was in an equatorial position, namely, a β–orientation.

The key NOE correlations of H–1β with H–3, H–3 with H–5, H–1α and H–11, H–1α with H–20, H–15 with H–7α, H–15 with H–14, indicated the orientation of H–3β, H–11α and H–15α. On the basis of the aforementioned evidence, the structure of 3 was determined, and the trivial name anthriscifolsine C was assigned to this compound.

HR–ESI–MS of anthriscifolrine A (4) gave a molecular ion at m/z 448.2757 [M + H]⁺ (calcd. for C₂₅H₃₈NO₆, 448.2699), corresponding to the molecular formula C₂₅H₃₇NO₆. Its NMR data indicated seven methylene (one oxymethylene), seven (two oxymethines), and five quaternary carbons (a carbonyl and two oxygen–bearing), in addition to a methylenedioxy group, an N–ethyl, and three methoxy substituents, suggesting that 4 was a typical lycoctonine C₁₉–diterpenoid alkaloid¹³. The 2D NMR and NOESY experiments confirmed the NMR data and configuration assignments of 4. In particular, HMBC correlations of C–14 with H–9, H–12, H–13, and H–16 confirmed the 14–keto group, while HMBC correlations of the protons of the methylenedioxy with C–7 and C–8, OCH₃–1 with C–1, OCH₃–16 with C–16, OCH₃–18 with C–18, confirmed the locations of the methylenedioxy and three methoxy groups. The α–oriented 1–OCH₃ and β–oriented 16–OCH₃ in compound 4 were deduced from the vicinal coupling constants (Table 2)and a NOESY experiment (Fig. 7). The structure of anthriscifolrine A was thus established.

Table 2 NMR Spectroscopic Data^a for Compounds 4–6 (600 MHz for ¹H, 150 MHz for ¹³C, CDCl_3, δ ppm).

Figure 7

Key ¹H–¹HCOSY, HMBC and NOSEY correlations of 4.

The molecular formula of anthriscifolrine B (5) was determined as C₂₇H₄₁NO₈ by HR–ESI–MS at m/z 508.2952 [M + H]⁺ (calcd. for C₂₇H₄₂NO₈, 508.2910). The NMR spectroscopic data of 5 were similar to those of 4, suggesting 5 was also a lycoctonine C₁₉–diterpenoid alkaloid with an acetyl group, an N–ethyl group, three methoxyl groups, and a methylenedioxy group¹³. In the HMBC spectrum of 5, critical correlations for the protons of the methylenedioxy/C–7 and C–8, OCH₃–1/C–1, OCH₃–16/C–16, OCH₃–18/C–18, H–14/OAc, suggested the location of three methoxyl groups at C–1, C–16 and C–18, the acetyl group at C–14, and the methylenedioxy group at C–7 and C–8. Its ¹³C NMR spectrum displayed seven oxygenated carbon signals, suggesting that it possessed an additional hydroxyl group in addition to three methoxy groups, an ester group, and a methylenedioxy group. The additional hydroxyl group in 5 was assigned to C–10 on the basis of the correlations of C–10 (δc 79.8) with H–1 (δ _H 3.57), H–9 (δ _H 2.42), H–13 (δ _H 2.76) and H–17 (δ _H 2.98) in the HMBC spectrum. The configurations of 1α–OCH₃, 16β–OCH₃, 14α–OAc and constant18β–OCH₃ were deduced by the vicinal coupling constants (Table 2) and a NOESY experiment. Thus, the structure of anthriscifolrine B was confirmed by NMR experiments.

The molecular formula of anthriscifolrine C (6) was determined as C₂₇H₄₁NO₉ (HR–ESI–MS). The ¹H and ¹³C NMR data (Table 2) of 6 showed close structural similarity to compound 5, and the distinction between the two sets of spectra was demonstrated by the presence of an additional hydroxyl group signal in 6, which was validated by the additional 16 mass units in mass spectrometry. The proton signal of H–6 at [δ _H 2.11 (1 H, m), 1.50 (1 H, m) and δ _C 32.8 (t)] in compound 5 was shifted downfield to [δ _H 5.52 (1 H, s), δ _C 78.3(d)] in compound 6, suggesting that the hydroxyl group in 6 might be located at C–6, which was further confirmed by the HMBC correlations. The hydroxyl group at C–6 was determined to have a β–orientation based on the multiplicity of H–6 (singlet) in the ¹H NMR spectrum². Thus, the structure of anthriscifolrine C was determined as shown in Fig. 1.

Anthriscifolrine D (7), a white amorphous powder, C₂₇H₃₉NO₉ (HR–ESI–MS), was also a lycoctonine–type C₁₉–diterpenoid alkaloid. By comparison of the NMR data of 7 with those of 4, the main difference was the presence of a acetyl and a hydroxyl groups. In the HMBC experiment, long–range correlations were observed from H–6 (5.54) to the carbonyl carbon of the acetyl group at δ _C 170.2, and H–1 (δ _H 3.91), H–9 (δ _H 3.49), H–13 (δ _H 2.82) and H–17 (δ _H 3.71) to C–10 (δc 79.8) supported the hydroxyl group at C–10. The structure of anthriscifolrine D (Fig. 1) was confirmed by the analysis of its 2D NMR data.

The molecular formula of anthriscifolrine E (8) was deduced to be C₂₆H₃₉NO₈ from its HR–ESI–MS at m/z 494.2752 [M + H]⁺. From its NMR data (Table 3), an N–ethyl group, two methoxy groups, an acetoxy group, and a methylenedioxy group could be easily recognized. Compound 8 shared highly similar ¹H– and ¹³C–NMR spectral patterns with those of 5. The only difference is that the absence of a methoxy group and the presence of a hydroxy group at C–18 in 8, which was further supported by comparison of the NMR data: the C–18 signal in 8 appeared at δ _C 68.3 instead of at δ _C 79.1 in 5. The structure of anthriscifolrine E was unquestionably confirmed by extensive analyses of its 1D and 2D NMR spectra.

Table 3 NMR Spectroscopic Data^a for Compounds 7–9 (600 MHz for ¹H, 150 MHz for ¹³C, CDCl_3, δ ppm).

Comparison of spectroscopic data of anthriscifolrine F (9) and E (8) indicated that an acetyl group in 8 was substituted by a methoxy group in 9. According to 2D NMR analysis, especially the HMBC correlation of OCH₃/C–14, the OCH₃ group was attributed to C–14 in 9. The corresponding structure of 9 was confirmed by DEPT, HMQC, ¹H–¹H COSY, and HMBC experiments. Thus, anthriscifolrine F was assigned as shown in Fig. 1.

To evaluate the biological activities of these compounds isolated from the whole plant of D.anthriscifolium var. majus, compounds 3–6 were tested for their in vitro cytotoxicity against the MCF–7, HepG2 and H460 cancer cell lines. Unfortunately, all of the compounds were inactive (IC₅₀ > 50 μM, n = 3).

Discussion

Investigation on the whole plant of Delphinium anthriscifolium var. majus resulted in the isolation of nine new diterpenoid alkaloids named anthriscifolsines A–C (1–3) and anthriscifolrines A–F (4–9), together with five known alkaloids (10–14). Notably, anthriscifolsine A (1) is the first naturally occurring C₂₀–diterpenoid alkaloid with a unique seco C–ring generated by an unprecedented C11–C12 bond cleavage, and its possible biogenetic pathway was proposed. Since the Sect. Anthriscifolium only comprises three species (D. anthriscifolium, D. anthriscifolium var. majus, and D. anthriscifolium var. savatieri), the present research would be particularly valuable in understanding their chemotaxonomical significance. The identification of various C₁₉– and C₂₀–diterpenoid alkaloids from D. anthriscifolium var. majus revealed its transitional position among the Delphinium plants.

Materials and Methods

General Experimental Procedures

Optical rotations were measured using a Perkin–Elmer 341 polarimeter. The IR spectra were obtained using a Thermo Fisher Nicolet 6700 spectrometer and KBr pellets in cm⁻¹. The HR–ESI–MS data were measured using a Q–TOF micro mass spectrometer (Waters). The 1D and 2D NMR spectra were recorded using a Bruker AV 600 with TMS. Silica gel (Qingdao Haiyang Chemical Co., Ltd., 200–300 mesh) was used for column chromatography (CC). The TLC plates were precoated with silica gel GF₂₅₄ (Qingdao Haiyang Chemical Co., Ltd., China), and it was visualized under a UV lamp at 254 nm or by spraying with Dragendorff’s reagent or iodine.

Plant Material

The whole herbs of D. anthriscifolium var. majus were collected in Longshanwa, Zhuxi county, Hubei province of China, in April 2015, and were identified (voucher specimen: L H. Shan & J X. Wang 801) by Prof. Qing–Er Yang of the Institute of Botany, Chinese Academy of Sciences.

Extraction and Isolation

Dried and powdered whole herbs of D. anthriscifolium (21.5 kg) were extracted with 95% EtOH four times at room temperature, with each soaking process lasting a week. After removal of the solvent by evaporation, the ethanol extract (2000 g) was recovered. The extract was suspended in H₂O (3 L) and adjusted to pH 2 with HCl, and successively extracted with petroleum ether (4 × 1 L) and ethyl acetate (4 × 1 L). The pH of aqueous layer was adjusted to 10 with aqueous ammonia solution and extracted with CH₂Cl₂ (4 × 1 L). The CH₂Cl₂ extracts were concentrated to produce the crude alkaloid extract (28.5 g). Column chromatography of the crude alkaloid extract over silica gel, using a CH₂Cl₂:MeOH (80:1, v/v) mixture with increasing polarity afforded fractions A–D based on TLC analysis.

Fraction A (10.7 g) was submitted to silica gel CC eluting with petroleum ether/Me₂CO/Et₂N (50: 1: 0.1 to 20:1:0.1, v/v/v) to yield compounds 4 (14 mg), 10 (20 mg) respectively.

Fraction B (7.2 g) was submitted to silica gel CC eluting with petroleum ether/Me₂CO/Et₂N (15: 1: 0.1 to 6:1:0.1, v/v/v) to yield compounds 5 (20 mg), 6 (43 mg) and 12 (8 mg).

Fraction C (5.6 g) was subjected to silica gel CC, petroleum ether/Me₂CO/Et₂N (15: 1: 0.1 to 6:1:0.1, v/v/v) to yield compounds 1 (16 mg), 11 (14 mg), 7 (10 mg) and 3 (4.9 mg).

Fraction D (5.0 g) was subjected to silica gel CC, eluted with CH₂Cl₂:MeOH (30:1 to 10:1, v/v) to get four fractions (D₁–D₄), fraction D₁ was subjected to Sephadex LH–20 column chromatography (MeOH) to yield compounds 13 (14 mg) and 14 (8 mg), fraction D₂ was further purified using an RP–18 silica gel column with MeOH: H₂O (10:90 to 30:70, v/v) as the mobile phase to yield compounds 2 (7.6 mg), 8 (20 mg), and 9 (11 mg).

Spectroscopic data of 1–9

Anthriscifolsine A (1): needle crystal (MeOH); ${[{\rm{\alpha }}]}_{{\rm{D}}}^{20}$ + 11.1 (c 0.38, CHCl₃); IR (KBr) v _max: 3465, 3070, 2928, 2880, 2854, 2747, 1744, 1716, 1667, 1630, 1450, 1381, 1340, 1276, 1233, 1121, 1062, 1038, 997, 965, 942, 909, 752, 715; ¹H and ¹³C NMR data see Table 1; HR–ESI–MS at m/z 506.2182 [M + H]⁺ (calcd. for C₂₉H₃₂NO₇, 506.2179).

Anthriscifolsine B (2): white, amorphous powder; ${[{\rm{\alpha }}]}_{{\rm{D}}}^{20}$ + 1.1 (c 0.38, CHCl₃); IR (KBr) v _max: 3408, 2925, 2853, 1741, 1655, 1371, 1251, 1063, 1042, 985, 769, 719; ¹H and ¹³C NMR data see Table 1; HR–ESI–MS at m/z 446.2196 [M + H]⁺ (calcd. for C₂₄H₃₂NO₇, 446.2179).

Anthriscifolsine C (3): white, amorphous powder; ${[{\rm{\alpha }}]}_{{\rm{D}}}^{20}$ −9.8 (c 0.25, CHCl₃); IR (KBr) v _max: 3443, 2967, 2935, 2878, 1727, 1656, 1462, 1383, 1265, 1237, 1187, 1152, 1077, 1135, 1009, 997, 906, 754, 715; ¹H and ¹³C NMR data see Table 1; HR–ESI–MS at m/z 530.3118 [M + H]⁺ (calcd. for C₃₀H₄₄NO₇, 530.3118).

Anthriscifolrine A (4): white, amorphous powder; ${[{\rm{\alpha }}]}_{{\rm{D}}}^{20}$ −4.2 (c 0.50, CHCl₃); IR (KBr) v _max: 3423, 2953, 2925, 2854, 1752, 1648, 1463, 1377, 1094, 954, 734, 721; ¹H and ¹³C NMR data see Table 2; HR–ESI–MS at m/z 448.2757 [M + H]⁺ (calcd. for C₂₅H₃₈NO₆, 448.2699).

Anthriscifolrine B (5): white, amorphous powder; ${[{\rm{\alpha }}]}_{{\rm{D}}}^{20}$ −26.7 (c 0.30, CHCl₃); IR (KBr) v _max: 3472, 2958, 2923, 2875, 2823, 2755, 1740, 1718, 1456, 1369, 1248, 1206, 1092, 1075, 1054, 961, 934, 755, 730; ¹H and ¹³C NMR data see Table 2; HR–ESI–MS at m/z 508.2952 [M + H]⁺ (calcd. for C₂₇H₄₂NO₈, 508.2910).

Anthriscifolrine C (6): white, amorphous powder; ${[{\rm{\alpha }}]}_{{\rm{D}}}^{20}$ −2.3 (c 0.70, CHCl₃); IR (KBr) v _max: 3438, 2972, 2927, 2875, 2829, 2750, 1738, 1666, 1453, 1387, 1368, 1246, 1232, 1090, 1045, 961, 918, 756, 715; ¹H and ¹³C NMR data see Table 2; HR–ESI–MS at m/z 524.2867 [M + H]⁺ (calcd. for C₂₇H₄₂NO₉, 524.2860).

Anthriscifolrine D (7): white, amorphous powder; ${[{\rm{\alpha }}]}_{{\rm{D}}}^{20}$ −12.8 (c 0.50, CHCl₃); IR (KBr) v _max: 3461, 2962, 2924, 2873, 2854, 2827, 2752, 1757, 1742, 1647, 1456, 1368, 1245, 1227, 1103, 1088, 1043, 958, 926, 761, 742; ¹H and ¹³C NMR data see Table 3; HR–ESI–MS at m/z 522.2702 [M + H]⁺ (calcd. for C₂₇H₄₀NO₉, 522.2703).

Anthriscifolrine E (8): white, amorphous powder; ${[{\rm{\alpha }}]}_{{\rm{D}}}^{20}$ −10.7 (c 0.30, CHCl₃); IR (KBr) v _max: 3447, 2962, 2926, 2885, 2857, 2818, 2746, 1741, 1717, 1463, 1370, 1265, 1230, 1092, 1075, 1052, 953, 913, 756, 729, 710; ¹H and ¹³C NMR data see Table 3; HR–ESI–MS at m/z 494.2752 [M + H]⁺ (calcd. for C₂₆H₄₀NO₈, 494.2754).

Anthriscifolrine F (9): white, amorphous powder; ${[{\rm{\alpha }}]}_{{\rm{D}}}^{20}$ −15.0 (c 0.30, CHCl₃); IR (KBr) v _max: 3528, 3382, 2958, 2925, 2889, 2855, 2817, 1742, 1666, 1464, 1388, 1371, 1327, 1239, 1158, 1132, 1045, 1103, 1087, 1072, 1051, 1001, 970, 955, 921, 781, 757, 731; ¹H and ¹³C NMR data see Table 3; HR–ESI–MS at m/z 466.2817 [M + H]⁺ (calcd. for C₂₅H₄₀NO₇, 466.2805).

Crystal Data of 1: C₂₉H₃₁NO₇, Mr 505.21, a = 12.9571(5) Å, b = 20.9703(17) Å, c = 21.7049(12) Å, V = 5897.5(6) ų, space group P2₁2₁2₁, Z = 8, D _calc = 1.204 Mg/m³, λ = 0.71073 Å, μ(Moka) = 0.086 mm⁻¹, F(000) = 2272.0, and T = 293.15 K; Data were collected using an orthorhombic of size 0.4 × 0.1 × 0.05 mm³ in the range −15 ≤ h ≤ 16, −24 ≤ k ≤ 26, −27 ≤ l ≤ 16. 21557 reflections measured, 11486 unique reflections R _int = 0.0297. Refinement by full–matrix least–squares on F ² converged to give final R indices R ₁ = 0.0781, wR ₂ = 0.1779 [I > 2σ(I)] and R ₁ = 0.1399, wR ₂ = 0.2106 (all data).

Data/restraints/parameters = 11486/1/725, goodness–of–fit on F ² = 0.988, largest difference peak and hole are 0.25 and −0.19 e Å⁻³. Crystallographic data for 1 have been deposited with the Cambridge Crystallographic Data Center as supplementary publication number CCDC 1487703. These data can be obtained free of charge via www.ccdc.cam.ac.uk/deposit (or from the CCDC, 12 Union Road, Cambridge CB2 1EZ, UK; fax: + 44 1223336033; deposit@ccdc.cam.ac.uk).

Crystal Data of 11: C₂₉H₃₁NO₇, Mr 505.21, a = 13.1758(7) Å, b = 18.3883(9) Å, c = 21.9022(13) Å, V = 5306.5(5) ų, space group P2₁2₁2₁, Z = 8, D _calc = 1.263 Mg/m³, λ = 0.71073 Å, μ(Moka) = 0.090 mm⁻¹, F(000) = 2136.0, and T = 293.15 K; Data were collected using an orthorhombic of size 0.4 × 0.08 × 0.08 mm³ in the range −16 ≤ h ≤ 15, −13 ≤ k ≤ 22, −23 ≤ l ≤ 27. 17628 reflections measured, 9715 unique reflections R _int = 0.0250. Refinement by full–matrix least–squares on F ² converged to give final R indices R ₁ = 0.0551, wR ₂ = 0.1345 [I > 2σ(I)] and R ₁ = 0.07969, wR ₂ = 0.1498 (all data).

Data/restraints/parameters = 9715/0/687, goodness–of–fit on F ² = 0.999, largest difference peak and hole are 0.36 and −0.14 e Å⁻³. Crystallographic data for 11 have been deposited with the Cambridge Crystallographic Data Center as supplementary publication number CCDC 1487702. These data can be obtained free of charge via www.ccdc.cam.ac.uk/deposit (or from the CCDC, 12 Union Road, Cambridge CB2 1EZ, UK; fax: + 44 1223336033; deposit@ccdc.cam.ac.u k).

Cell Culture and Cytotoxicity Assay

The cytotoxicity of the compounds against cultured human tumor cell lines such as MCF–7, HepG2 and H460 cell lines was evaluated by the MTT method as described in our previous paper¹⁴. Cells treated with DMSO (0.1% v/v) were used as negative controls, whereas adriamycin (≥98%; Sigma Chemical Co., Ltd., Shanghai, China) was used as the positive control.