Figure 4 | Scientific Reports

Figure 4

From: Progressive Changes in CXCR4 Expression That Define Thymocyte Positive Selection Are Dispensable For Both Innate and Conventional αβT-cell Development

Figure 4

Intrathymic iNKT cell Development In CXCR4 cKO Mice. (a) Representative expression of CXCR4 by PBS-57+ CD1d-restricted thymocytes from WT mice. (b) Representative expression of CXCR4 by populations of PBS57+ thymic iNKT cells, defined as CD44NK1.1CD24+, CD44NK1.1CD24, CD44+NK1.1, and CD44+NK1.1+. Grey histograms show isotype control staining for CXCR4. (c) FACS analysis of total and iNKT cell subsets in control CD4Cre and CXCR4 cKO mice. (d) Quantitation of total and iNKT cell subsets in control CD4Cre and CXCR4 cKO mice. (e) TCR Vα14-Jα18 rearrangements in PBS-57+ depleted DP thymocytes from control or CXCR4 cKO mice. All quantitation is generated from at least three independent experiments where n > 7, unpaired Student’s t test shows no significant difference. Error bars represent SEM.