Figure 3 | Scientific Reports

Figure 3

From: Progressive Changes in CXCR4 Expression That Define Thymocyte Positive Selection Are Dispensable For Both Innate and Conventional αβT-cell Development

Figure 3

Conventional αβT-cell Development Occurs Normally In CXCR4 cKO Mice. (a) Immunofluorescence showing typical positioning of thymocytes in CD4Cre and CXCR4 cKO mice, ‘C’ denotes cortex and ‘M’ denotes medulla, scale bar represents 100 μm. (b) Thymus cellularity of CXCR4 cKO compared to CD4Cre mice. Representative FACS analysis and quantitation of (c) DP and SP thymocytes, (d) TCRβlo, TCRβint and TCRβhi DP thymocytes, (e) CD25+Foxp3+ T-Reg, (f) CD69+CD62L immature and CD69CD62L+ mature SP4 and SP8 thymocytes from CXCR4 cKO and CD4Cre control thymus. (g) Spleen cellularity and numbers of CD4, CD8 and Foxp3+ T cells in the spleen. (h) Numbers of naïve (TN CD62L+CD44), central memory (TCM CD62L+CD44+) and effector memory (CD62LCD44+) CD4+ and CD8+ T cell subsets in the spleen of CD4Cre and CXCR4 cKO mice. All quantitation is generated from at least three independent experiments where n > 7, unpaired Student’s t test shows no significant difference. Error bars represent SEM.